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. 1997 Apr;41(4):838–840. doi: 10.1128/aac.41.4.838

Emergence of an inhibitor-resistant beta-lactamase (SHV-10) derived from an SHV-5 variant.

E E Prinarakis 1, V Miriagou 1, E Tzelepi 1, M Gazouli 1, L S Tzouvelekis 1
PMCID: PMC163805  PMID: 9087500

Abstract

An inhibitor-resistant beta-lactamase (SHV-10), derived from an SHV-5 variant (SHV-9), was found in an Escherichia coli clinical isolate. In SHV-10, Ser-130 was replaced by Gly. The enzyme partially retained its ability to hydrolyze penicillins, but its activity against cephalosporins was drastically reduced. A Ser-130-->Gly mutant of the prototype SHV-5, obtained by site-directed mutagenesis, had properties similar to those of SHV-10.

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Selected References

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