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. 1979 Dec;33:17–23. doi: 10.1289/ehp.793317

Methods for the analysis of intestinal function.

C T Walsh, R R Levine
PMCID: PMC1638115  PMID: 540616

Abstract

The intestinal tract, an organ of considerable complexity, requires application of numerous techniques for analysis of its physiology and perturbations by toxicologic agents. This review describes the methodology of importance in analysis of the absorptive function of the intestine and the transit of intestinal contents. Methods for studying absorption are categorized according to the technique for administering the test substance such as inclusion in the diet or by gastric and intestinal placement and the method of quantitating the degree of absorption such as determining the appearance of a test substance in systemic fluids or its disappearance from its site of administration in the intestine. In vitro techniques which have no in vivo analogs, such as the use of the everted sac, are briefly described and their limitations emphasized. Procedures of importance in the clinical diagnosis of malabsorption or in the experimental analysis of absorptive function in man are included and distinguished from techniques used in animal models. In addition, methods for studying aspects of gastrointestinal motility, including the use of luminal markers and analysis of the contractile and electrical activity of intestinal smooth muscle, are reviewed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barr R. G., Perman J. A., Schoeller D. A., Watkins J. B. Breath tests in pediatric gastrointestinal disorders: new diagnostic opportunities. Pediatrics. 1978 Sep;62(3):393–401. [PubMed] [Google Scholar]
  2. Barr W. H., Riegelman S. Intestinal drug absorption and metabolism. I. Comparison of methods and models to study physiological factors of in vitro and in vivo intestinal absorption. J Pharm Sci. 1970 Feb;59(2):154–163. doi: 10.1002/jps.2600590204. [DOI] [PubMed] [Google Scholar]
  3. CRANE R. K., MANDELSTAM P. The active transport of sugars by various preparations of hamster intestine. Biochim Biophys Acta. 1960 Dec 18;45:460–476. doi: 10.1016/0006-3002(60)91482-7. [DOI] [PubMed] [Google Scholar]
  4. CRANE R. K., WILSON T. H. In vitro method for the study of the rate of intestinal absorption of sugars. J Appl Physiol. 1958 Jan;12(1):145–146. doi: 10.1152/jappl.1958.12.1.145. [DOI] [PubMed] [Google Scholar]
  5. Dennhardt R., Lingelbach B., Haberich F. J. Intestinal absorption under the influence of vasopressin: studies in unanaesthetised rats. Gut. 1979 Feb;20(2):107–113. doi: 10.1136/gut.20.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fordtran J. S., Rector F. C., Jr, Ewton M. F., Soter N., Kinney J. Permeability characteristics of the human small intestine. J Clin Invest. 1965 Dec;44(12):1935–1944. doi: 10.1172/JCI105299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gibaldi M., Boyes R. N., Feldman S. Influence of first-pass effect on availability of drugs on oral administration. J Pharm Sci. 1971 Sep;60(9):1338–1340. doi: 10.1002/jps.2600600909. [DOI] [PubMed] [Google Scholar]
  8. Gibaldi M., Grundhofer B. Drug transport. VI. Functional integrity of the rat everted small intestine with respect to passive transfer. J Pharm Sci. 1972 Jan;61(1):116–118. doi: 10.1002/jps.2600610125. [DOI] [PubMed] [Google Scholar]
  9. Hepner G. W. Breath tests in gastroenterology. Adv Intern Med. 1978;23:25–45. [PubMed] [Google Scholar]
  10. Hohenleitner F. J., Senior J. R. Metabolism of canine small intestine vascularly perfused in vitro. J Appl Physiol. 1969 Jan;26(1):119–128. doi: 10.1152/jappl.1969.26.1.119. [DOI] [PubMed] [Google Scholar]
  11. Hopfer U. INtestinal sugar transport: studies with isolated plasma membranes. Ann N Y Acad Sci. 1975 Dec 30;264:414–427. doi: 10.1111/j.1749-6632.1975.tb31500.x. [DOI] [PubMed] [Google Scholar]
  12. LEVINE R. R., PELIKAN E. W. The influence of experimental procedures and dose on the intestinal absorption of an onium compound, benzomethamine. J Pharmacol Exp Ther. 1961 Mar;131:319–327. [PubMed] [Google Scholar]
  13. Lavigne M. E., Wiley Z. D., Meyer J. H., Martin P., MacGregor I. L. Gastric emptying rates of solid food in relation to body size. Gastroenterology. 1978 Jun;74(6):1258–1260. [PubMed] [Google Scholar]
  14. Levine P. H., Weintraub L. R. Preparation of suspensions of small bowel mucosal epithelial cells. J Lab Clin Med. 1970 Jun;75(6):1026–1029. [PubMed] [Google Scholar]
  15. Levine R. R., McNary W. F., Kornguth P. J., LeBlanc R. Histological reevaluation of everted gut technique for studying intestinal absorption. Eur J Pharmacol. 1970 Feb;9(2):211–219. doi: 10.1016/0014-2999(70)90302-x. [DOI] [PubMed] [Google Scholar]
  16. Lewis L. D., Fordtran J. S. Effect of perfusion rate on absorption, surface area, unstirred water layer thickness, permeability, and intraluminal pressure in the rat ileum in vivo. Gastroenterology. 1975 Jun;68(6):1509–1516. [PubMed] [Google Scholar]
  17. Pelzmann K. S., Havemeyer R. N. Portal vein blood sampling in intestinal drug absorption studies. J Pharm Sci. 1971 Feb;60(2):331–332. doi: 10.1002/jps.2600600248. [DOI] [PubMed] [Google Scholar]
  18. Schwabe A. D., Hepner G. W. Breath tests for the detection of fat malabsorption. Gastroenterology. 1979 Jan;76(1):216–218. [PubMed] [Google Scholar]
  19. Scott L. D., Summers R. W. Correlation of contractions and transit in rat small intestine. Am J Physiol. 1976 Jan;230(1):132–137. doi: 10.1152/ajplegacy.1976.230.1.132. [DOI] [PubMed] [Google Scholar]
  20. Soergel K. H. Inert markers. Gastroenterology. 1968 Mar;54(3):449–452. [PubMed] [Google Scholar]
  21. Solomons N. W., Schoeller D. A., Wagonfeld J. B., Ott D., Rosenberg I. H., Klein P. D. Application of a stable isotope (13C)-labeled glycocholate breath test to diagnosis of bacterial overgrowth and ileal dysfunction. J Lab Clin Med. 1977 Sep;90(3):431–439. [PubMed] [Google Scholar]
  22. Summers R. W., Kent T. H., Osborne J. W. Effects of drugs, ileal obstruction, and irradiation on rat gastrointestinal propulsion. Gastroenterology. 1970 Nov;59(5):731–739. [PubMed] [Google Scholar]
  23. Towler C. M., Pugh-Humphreys G. P., Porteous J. W. Characterization of columnar absorptive epithelial cells isolated from rat jejunum. J Cell Sci. 1978 Feb;29:53–75. doi: 10.1242/jcs.29.1.53. [DOI] [PubMed] [Google Scholar]
  24. Trier J. S. Organ-culture methods in the study of gastrointestinal-mucosal function and development. N Engl J Med. 1976 Jul 15;295(3):150–155. doi: 10.1056/NEJM197607152950308. [DOI] [PubMed] [Google Scholar]
  25. WILSON T. H., WISEMAN G. The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. J Physiol. 1954 Jan;123(1):116–125. doi: 10.1113/jphysiol.1954.sp005036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Watkins J. B., Schoeller D. A., Klein P. D., Ott D. G., Newcomer A. D., Hofmann A. F. 13C-trioctanoin: a nonradioactive breath test to detect fat malabsorption. J Lab Clin Med. 1977 Sep;90(3):422–430. [PubMed] [Google Scholar]
  27. Wolfe D. L., Forland S. C., Benet L. Z. Drug transfer across intact rat intestinal mucosa following surgical removal of serosa and muscularis externa. J Pharm Sci. 1973 Feb;62(2):200–205. doi: 10.1002/jps.2600620203. [DOI] [PubMed] [Google Scholar]

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