Abstract
Factor-VIII-related antigen was measured, both by an enzyme immunoassay using a microplate method and by the Laurell technique, in normal people, patients with von Willebrand's disease, haemophiliacs, and obligatory haemophilia carriers. The enzyme immunoassay was simpler to perform and gave equally reliable and reproducible results. Many more assays could be carried out at any one time.
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Selected References
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- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Hoyer L. W. Immunologic studies of antihemophilic factor (AHF, factor VIII). IV. Radioimmunoassay of AHF antigen. J Lab Clin Med. 1972 Dec;80(6):822–833. [PubMed] [Google Scholar]
- Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
- Voller A., Draper C., Bidwell D. E., Bartlett A. Microplate enzyme-linked immunosorbent assay for chagas' disease. Lancet. 1975 Feb 22;1(7904):426–428. doi: 10.1016/s0140-6736(75)91492-0. [DOI] [PubMed] [Google Scholar]
- Zimmerman T. S., Ratnoff O. D., Littell A. S. Detection of carriers of classic hemophilia using an immunologic assay for antihemophilic factor (factor 8(). J Clin Invest. 1971 Jan;50(1):255–258. doi: 10.1172/JCI106481. [DOI] [PMC free article] [PubMed] [Google Scholar]