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. 1997 Jul;41(7):1598–1600. doi: 10.1128/aac.41.7.1598

Antibiotic resistance among enterococci isolated from clinical specimens between 1953 and 1954.

B A Atkinson 1, A Abu-Al-Jaibat 1, D J LeBlanc 1
PMCID: PMC163967  PMID: 9210693

Abstract

Two hundred twenty group D streptococci isolated from 1953 to 1954 from patients in the Washington, D.C., area were characterized. All were susceptible to ampicillin, vancomycin, and gentamicin; none produced beta-lactamase activity. High-level resistance to streptomycin was expressed by 117 strains, and 2 strains were resistant to >8 microg of chloramphenicol per ml. Three isolates were resistant to both erythromycin and lincomycin, and DNA from these hybridized to an ermAM probe. Of 118 strains resistant to tetracycline and minocycline, genomic DNA from 63 was examined for homology to tet(M), tet(O), and tet(S). DNA from 20 strains hybridized to tet(M), DNA from 37 strains hybridized to tet(S), and DNA from none of the strains hybridized to tet(O).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burdett V., Inamine J., Rajagopalan S. Heterogeneity of tetracycline resistance determinants in Streptococcus. J Bacteriol. 1982 Mar;149(3):995–1004. doi: 10.1128/jb.149.3.995-1004.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burdett V. Nucleotide sequence of the tet(M) gene of Tn916. Nucleic Acids Res. 1990 Oct 25;18(20):6137–6137. doi: 10.1093/nar/18.20.6137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Charpentier E., Gerbaud G., Courvalin P. Characterization of a new class of tetracycline-resistance gene tet(S) in Listeria monocytogenes BM4210. Gene. 1993 Sep 6;131(1):27–34. doi: 10.1016/0378-1119(93)90665-p. [DOI] [PubMed] [Google Scholar]
  5. Charpentier E., Gerbaud G., Courvalin P. Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis. Antimicrob Agents Chemother. 1994 Oct;38(10):2330–2335. doi: 10.1128/aac.38.10.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Facklam R. R., Collins M. D. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J Clin Microbiol. 1989 Apr;27(4):731–734. doi: 10.1128/jcm.27.4.731-734.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JONES W. F., Jr, FINLAND M. Susceptibility of enterococcus to eleven antibiotics in vitro; with special reference to species differences. Am J Clin Pathol. 1957 Apr;27(4):467–481. doi: 10.1093/ajcp/27.4_ts.467. [DOI] [PubMed] [Google Scholar]
  8. LeBlanc D. J., Inamine J. M., Lee L. N. Broad geographical distribution of homologous erythromycin, kanamycin, and streptomycin resistance determinants among group D streptococci of human and animal origin. Antimicrob Agents Chemother. 1986 Apr;29(4):549–555. doi: 10.1128/aac.29.4.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LeBlanc D. J., Lee L. N. Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1. J Bacteriol. 1982 May;150(2):835–843. doi: 10.1128/jb.150.2.835-843.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LeBlanc D. J., Lee L. N., Titmas B. M., Smith C. J., Tenover F. C. Nucleotide sequence analysis of tetracycline resistance gene tetO from Streptococcus mutans DL5. J Bacteriol. 1988 Aug;170(8):3618–3626. doi: 10.1128/jb.170.8.3618-3626.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Novak K. F., LeBlanc D. J. Characterization of plasmid pVT745 isolated from Actinobacillus actinomycetemcomitans. Plasmid. 1994 Jan;31(1):31–39. doi: 10.1006/plas.1994.1004. [DOI] [PubMed] [Google Scholar]
  12. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  13. Toala P., McDonald A., Wilcox C., Finland M. Comparison of antibiotic susceptibility of group D streptococcus strains isolated at Boston City Hospital in 1953-54 and 1968-69. Antimicrob Agents Chemother (Bethesda) 1969;9:479–484. [PubMed] [Google Scholar]
  14. Toala P., McDonald A., Wilcox C., Finland M. Susceptibility of group D streptococcus (enterococcus) to 21 antibiotics in vitro, with special reference to species differences. Am J Med Sci. 1969 Dec;258(6):416–430. doi: 10.1097/00000441-196912000-00006. [DOI] [PubMed] [Google Scholar]
  15. Weisblum B. Erythromycin resistance by ribosome modification. Antimicrob Agents Chemother. 1995 Mar;39(3):577–585. doi: 10.1128/AAC.39.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]

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