Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1997 Jul;41(7):1604–1606. doi: 10.1128/aac.41.7.1604

Cross-resistance associated with development of resistance to isometamidium in a clone of Trypanosoma congolense.

A S Peregrine 1, M A Gray 1, S K Moloo 1
PMCID: PMC163969  PMID: 9210695

Abstract

Resistance to isometamidium was increased 94-fold in a clone of Trypanosoma congolense (clone IL 1180) by repeated subcurative treatment of infected mice for 11 months. This was associated with 3.4-, 33-, and 4.2-fold increases in resistance to diminazene, homidium, and quinapyramine, respectively. Both T. congolense IL 1180 and the resistant derivative were able to undergo cyclical development in Glossina morsitans centralis tsetse flies, producing hypopharyngeal infection rates of 40.0 and 39.8%, respectively.

Full Text

The Full Text of this article is available as a PDF (104.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clausen P. H., Sidibe I., Kaboré I., Bauer B. Development of multiple drug resistance of Trypanosoma congolense in Zebu cattle under high natural tsetse fly challenge in the pastoral zone of Samorogouan, Burkina Faso. Acta Trop. 1992 Aug;51(3-4):229–236. doi: 10.1016/0001-706x(92)90041-u. [DOI] [PubMed] [Google Scholar]
  2. Codjia V., Mulatu W., Majiwa P. A., Leak S. G., Rowlands G. J., Authié E., d'Ieteren G. D., Peregrine A. S. Epidemiology of bovine trypanosomiasis in the Ghibe valley, southwest Ethiopia. 3. Occurrence of populations of Trypanosoma congolense resistant to diminazene, isometamidium and homidium. Acta Trop. 1993 Apr;53(2):151–163. doi: 10.1016/0001-706x(93)90026-8. [DOI] [PubMed] [Google Scholar]
  3. Dar F. K., Ligthart G. S., Wilson A. J. Cryopreservation of pathogenic African trypanosomes in situ: metacyclic and bloodstream forms. J Protozool. 1972 Aug;19(3):494–497. doi: 10.1111/j.1550-7408.1972.tb03511.x. [DOI] [PubMed] [Google Scholar]
  4. Geigy R., Kauffmann M. Sleeping sickness survey in the Serengeti area (Tanzania) 1971. I. Examination of large mammals for trypanosomes. Acta Trop. 1973;30(1):12–23. [PubMed] [Google Scholar]
  5. Gray M. A., Peregrine A. S. An in vitro assay for drug sensitivity of Trypanosoma congolense using in vitro-derived metacyclic trypanosomes. Acta Trop. 1993 Sep;54(3-4):291–300. doi: 10.1016/0001-706x(93)90101-g. [DOI] [PubMed] [Google Scholar]
  6. Kinabo L. D., Bogan J. A. The pharmacology of isometamidium. J Vet Pharmacol Ther. 1988 Sep;11(3):233–245. doi: 10.1111/j.1365-2885.1988.tb00148.x. [DOI] [PubMed] [Google Scholar]
  7. Leach T. M., Roberts C. J. Present status of chemotherapy and chemoprophylaxis of animal trypanosomiasis in the Eastern hemisphere. Pharmacol Ther. 1981;13(1):91–147. doi: 10.1016/0163-7258(81)90069-3. [DOI] [PubMed] [Google Scholar]
  8. Mohamed-Ahmed M. M., Rahman A. H., Abdel Karim E. I. Multiple drug-resistant bovine trypanosomes in South Darfur Province, Sudan. Trop Anim Health Prod. 1992 Aug;24(3):179–181. doi: 10.1007/BF02359614. [DOI] [PubMed] [Google Scholar]
  9. Mulugeta W., Wilkes J., Mulatu W., Majiwa P. A., Masake R., Peregrine A. S. Long-term occurrence of Trypanosoma congolense resistant to diminazene, isometamidium and homidium in cattle at Ghibe, Ethiopia. Acta Trop. 1997 Apr 15;64(3-4):205–217. doi: 10.1016/s0001-706x(96)00645-6. [DOI] [PubMed] [Google Scholar]
  10. Murray M., Murray P. K., McIntyre W. I. An improved parasitological technique for the diagnosis of African trypanosomiasis. Trans R Soc Trop Med Hyg. 1977;71(4):325–326. doi: 10.1016/0035-9203(77)90110-9. [DOI] [PubMed] [Google Scholar]
  11. Nantulya V. M., Musoke A. J., Rurangirwa F. R., Moloo S. K. Resistance of cattle to tsetse-transmitted challenge with Trypanosoma brucei or Trypanosoma congolense after spontaneous recovery from syringe-passaged infections. Infect Immun. 1984 Feb;43(2):735–738. doi: 10.1128/iai.43.2.735-738.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ndoutamia G., Moloo S. K., Murphy N. B., Peregrine A. S. Derivation and characterization of a quinapyramine-resistant clone of Trypanosoma congolense. Antimicrob Agents Chemother. 1993 May;37(5):1163–1166. doi: 10.1128/aac.37.5.1163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Peregrine A. S. Chemotherapy and delivery systems: haemoparasites. Vet Parasitol. 1994 Aug;54(1-3):223–248. doi: 10.1016/0304-4017(94)90092-2. [DOI] [PubMed] [Google Scholar]
  14. Peregrine A. S., Knowles G., Ibitayo A. I., Scott J. R., Moloo S. K., Murphy N. B. Variation in resistance to isometamidium chloride and diminazene aceturate by clones derived from a stock of Trypanosoma congolense. Parasitology. 1991 Feb;102(Pt 1):93–100. doi: 10.1017/s0031182000060388. [DOI] [PubMed] [Google Scholar]
  15. Schönefeld A., Röttcher D., Moloo S. K. The sensitivity to trypanocidal drugs of Trypanosoma vivax isolated in Kenya and Somalia. Trop Med Parasitol. 1987 Sep;38(3):177–180. [PubMed] [Google Scholar]
  16. Shapiro T. A., Englund P. T. Selective cleavage of kinetoplast DNA minicircles promoted by antitrypanosomal drugs. Proc Natl Acad Sci U S A. 1990 Feb;87(3):950–954. doi: 10.1073/pnas.87.3.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sones K. R., Holmes P. H., Urquhart G. M. Interference between drug-resistant and drug-sensitive stocks of Trypanosoma congolense in goats. Res Vet Sci. 1989 Jul;47(1):75–77. [PubMed] [Google Scholar]
  18. Sutherland I. A., Mounsey A., Holmes P. H. Transport of isometamidium (Samorin) by drug-resistant and drug-sensitive Trypanosoma congolense. Parasitology. 1992 Jun;104(Pt 3):461–467. doi: 10.1017/s0031182000063721. [DOI] [PubMed] [Google Scholar]
  19. Wilkes J. M., Peregrine A. S., Zilberstein D. The accumulation and compartmentalization of isometamidium chloride in Trypanosoma congolense, monitored by its intrinsic fluorescence. Biochem J. 1995 Nov 15;312(Pt 1):319–327. doi: 10.1042/bj3120319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zilberstein D., Wilkes J., Hirumi H., Peregrine A. S. Fluorescence analysis of the interaction of isometamidium with Trypanosoma congolense. Biochem J. 1993 May 15;292(Pt 1):31–35. doi: 10.1042/bj2920031. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES