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. 1997 Sep;41(9):1991–1995. doi: 10.1128/aac.41.9.1991

Pharmacokinetics of florfenicol in cerebrospinal fluid and plasma of calves.

B A de Craene 1, P Deprez 1, E D'Haese 1, H J Nelis 1, W Van den Bossche 1, P De Leenheer 1
PMCID: PMC164050  PMID: 9303399

Abstract

Florfenicol, a fluorinated analog of thiamphenicol, is of great value in veterinary infectious diseases that formerly responded favorably to chloramphenicol. In view of the treatment of meningitis in calves, we studied its pharmacokinetics in the cerebrospinal fluid (CSF) and plasma of six animals. To this end, a new high-performance liquid chromatography method was developed which, unlike previous ones, uses solid-phase instead of double-phase extraction to isolate the drug. After a single intravenous dose of 20 mg/kg of body weight, a maximum concentration in CSF of 4.67 +/- 1.51 microg/ml (n = 6) was reached, with a mean residence time of 8.7 h. The decline of florfenicol in both CSF and plasma fitted a biexponential model with elimination half-lives of 13.4 and 3.2 h, respectively. Florfenicol penetrated well into CSF, as evidenced from an availability of 46% +/- 3% relative to plasma. The levels remained above the MIC for Haemophilus somnus over a 20-h period. Our results provide evidence indicating the effectiveness of florfenicol in the treatment of bacterial meningitis of calves.

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Selected References

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  1. Adams P. E., Varma K. J., Powers T. E., Lamendola J. F. Tissue concentrations and pharmacokinetics of florfenicol in male veal calves given repeated doses. Am J Vet Res. 1987 Dec;48(12):1725–1732. [PubMed] [Google Scholar]
  2. Bretzlaff K. N., Neff-Davis C. A., Ott R. S., Koritz G. D., Gustafsson B. K., Davis L. E. Florfenicol in non-lactating dairy cows: pharmacokinetics, binding to plasma proteins, and effects on phagocytosis by blood neutrophils. J Vet Pharmacol Ther. 1987 Sep;10(3):233–240. doi: 10.1111/j.1365-2885.1987.tb00534.x. [DOI] [PubMed] [Google Scholar]
  3. Hormazabal V., Steffenak I., Yndestad M. Simultaneous determination of residues of florfenicol and the metabolite florfenicol amine in fish tissues by high-performance liquid chromatography. J Chromatogr. 1993 Jun 23;616(1):161–165. doi: 10.1016/0378-4347(93)80484-l. [DOI] [PubMed] [Google Scholar]
  4. Lobell R. D., Varma K. J., Johnson J. C., Sams R. A., Gerken D. F., Ashcraft S. M. Pharmacokinetics of florfenicol following intravenous and intramuscular doses to cattle. J Vet Pharmacol Ther. 1994 Aug;17(4):253–258. doi: 10.1111/j.1365-2885.1994.tb00241.x. [DOI] [PubMed] [Google Scholar]
  5. Neu H. C., Fu K. P. In vitro activity of chloramphenicol and thiamphenicol analogs. Antimicrob Agents Chemother. 1980 Aug;18(2):311–316. doi: 10.1128/aac.18.2.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Proost J. H., Meijer D. K. MW/Pharm, an integrated software package for drug dosage regimen calculation and therapeutic drug monitoring. Comput Biol Med. 1992 May;22(3):155–163. doi: 10.1016/0010-4825(92)90011-b. [DOI] [PubMed] [Google Scholar]
  7. Sande M. A. Antibiotic therapy of bacterial meningitis: lessons we've learned. Am J Med. 1981 Oct;71(4):507–510. doi: 10.1016/0002-9343(81)90191-1. [DOI] [PubMed] [Google Scholar]
  8. Scheer M., Förster D. Infektiöse septikämisch-thrombosierende Meningoenzephalitis in einem Mastbullen-Bestand. III. Therapieversuche mit Ampicillin (Binotal) Dtsch Tierarztl Wochenschr. 1975 Jul 5;82(7):267–269. [PubMed] [Google Scholar]
  9. Scott P. R., Penny C. D. A field study of meningoencephalitis in calves with particular reference to analysis of cerebrospinal fluid. Vet Rec. 1993 Jul 31;133(5):119–121. doi: 10.1136/vr.133.5.119. [DOI] [PubMed] [Google Scholar]
  10. Sisodia C. S., Dunlop R. H., Gupta V. S., Taksas L. A pharmacologic study of chloramphenicol in cattle. Am J Vet Res. 1973 Sep;34(9):1147–1151. [PubMed] [Google Scholar]
  11. Soback S., Paape M. J., Filep R., Varma K. J. Florfenicol pharmacokinetics in lactating cows after intravenous, intramuscular and intramammary administration. J Vet Pharmacol Ther. 1995 Dec;18(6):413–417. doi: 10.1111/j.1365-2885.1995.tb00618.x. [DOI] [PubMed] [Google Scholar]
  12. Syriopoulou V. P., Harding A. L., Goldmann D. A., Smith A. L. In vitro antibacterial activity of fluorinated analogs of chloramphenicol and thiamphenicol. Antimicrob Agents Chemother. 1981 Feb;19(2):294–297. doi: 10.1128/aac.19.2.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Täuber M. G., Zak O., Scheld W. M., Hengstler B., Sande M. A. The postantibiotic effect in the treatment of experimental meningitis caused by Streptococcus pneumoniae in rabbits. J Infect Dis. 1984 Apr;149(4):575–583. doi: 10.1093/infdis/149.4.575. [DOI] [PubMed] [Google Scholar]
  14. Varma K. J., Adams P. E., Powers T. E., Powers J. D., Lamendola J. F. Pharmacokinetics of florfenicol in veal calves. J Vet Pharmacol Ther. 1986 Dec;9(4):412–425. doi: 10.1111/j.1365-2885.1986.tb00062.x. [DOI] [PubMed] [Google Scholar]

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