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. 1997 Oct;41(10):2302–2304. doi: 10.1128/aac.41.10.2302

Involvement of a 43-kilodalton outer membrane protein in beta-lactam resistance of Shigella dysenteriae.

A K Kar 1, A S Ghosh 1, K Chauhan 1, J Ahamed 1, J Basu 1, P Chakrabarti 1, M Kundu 1
PMCID: PMC164115  PMID: 9333070

Abstract

A beta-lactam-sensitive strain (C152) of Shigella dysenteriae showed two major outer membrane proteins (OMPs) with M(r)s of 43,000 and 38,000, while the clinical isolate M2 lacked the 43,000-Mr OMP, which acted as a channel for beta-lactam antibiotics. Permeability of beta-lactams across the outer membrane (OM) of M2 was lower than that across the OM of C152. Mutants deficient in the 43-kDa OMP could be selected in vitro from strain C152 in the presence of cefoxitin. All beta-lactam-resistant strains were sensitive to imipenem.

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Selected References

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  1. Albertí S., Rodríquez-Quiñones F., Schirmer T., Rummel G., Tomás J. M., Rosenbusch J. P., Benedí V. J. A porin from Klebsiella pneumoniae: sequence homology, three-dimensional model, and complement binding. Infect Immun. 1995 Mar;63(3):903–910. doi: 10.1128/iai.63.3.903-910.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennish M. L., Salam M. A. Rethinking options for the treatment of shigellosis. J Antimicrob Chemother. 1992 Sep;30(3):243–247. doi: 10.1093/jac/30.3.243. [DOI] [PubMed] [Google Scholar]
  3. Charrel R. N., Pagès J. M., De Micco P., Mallea M. Prevalence of outer membrane porin alteration in beta-lactam-antibiotic-resistant Enterobacter aerogenes. Antimicrob Agents Chemother. 1996 Dec;40(12):2854–2858. doi: 10.1128/aac.40.12.2854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen M. L. Antimicrobial resistance: prognosis for public health. Trends Microbiol. 1994 Oct;2(10):422–425. doi: 10.1016/0966-842x(94)90623-8. [DOI] [PubMed] [Google Scholar]
  5. Harder K. J., Nikaido H., Matsuhashi M. Mutants of Escherichia coli that are resistant to certain beta-lactam compounds lack the ompF porin. Antimicrob Agents Chemother. 1981 Oct;20(4):549–552. doi: 10.1128/aac.20.4.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Komatsu Y., Murakami K., Nishikawa T. Penetration of moxalactam into its target proteins in Escherichia coli K-12: comparison of a highly moxalactam resistant mutant with its parent strain. Antimicrob Agents Chemother. 1981 Nov;20(5):613–619. doi: 10.1128/aac.20.5.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Lee E. H., Collatz E., Trias J., Gutmann L. Diffusion of beta-lactam antibiotics into proteoliposomes reconstituted with outer membranes of isogenic imipenem-susceptible and -resistant strains of Enterobacter cloacae. J Gen Microbiol. 1992 Nov;138(11):2347–2351. doi: 10.1099/00221287-138-11-2347. [DOI] [PubMed] [Google Scholar]
  9. Lee E. H., Nicolas M. H., Kitzis M. D., Pialoux G., Collatz E., Gutmann L. Association of two resistance mechanisms in a clinical isolate of Enterobacter cloacae with high-level resistance to imipenem. Antimicrob Agents Chemother. 1991 Jun;35(6):1093–1098. doi: 10.1128/aac.35.6.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Livermore D. M. beta-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev. 1995 Oct;8(4):557–584. doi: 10.1128/cmr.8.4.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
  12. Mahapatra S., Basu J., van Beeumen J., Kundu M. Characterization of a 38 kDa penicillin-binding protein and its possible involvement in maintaining stationary-phase cells of Shigella dysenteriae. Microbiology. 1994 Nov;140(Pt 11):3177–3182. doi: 10.1099/13500872-140-11-3177. [DOI] [PubMed] [Google Scholar]
  13. Martínez-Martínez L., Hernández-Allés S., Albertí S., Tomás J. M., Benedi V. J., Jacoby G. A. In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum-cephalosporins. Antimicrob Agents Chemother. 1996 Feb;40(2):342–348. doi: 10.1128/aac.40.2.342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Masuda N., Sakagawa E., Ohya S. Outer membrane proteins responsible for multiple drug resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1995 Mar;39(3):645–649. doi: 10.1128/AAC.39.3.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nakae T. Role of membrane permeability in determining antibiotic resistance in Pseudomonas aeruginosa. Microbiol Immunol. 1995;39(4):221–229. doi: 10.1111/j.1348-0421.1995.tb02193.x. [DOI] [PubMed] [Google Scholar]
  16. Nikaido H. Multidrug efflux pumps of gram-negative bacteria. J Bacteriol. 1996 Oct;178(20):5853–5859. doi: 10.1128/jb.178.20.5853-5859.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nikaido H., Normark S. Sensitivity of Escherichia coli to various beta-lactams is determined by the interplay of outer membrane permeability and degradation by periplasmic beta-lactamases: a quantitative predictive treatment. Mol Microbiol. 1987 Jul;1(1):29–36. doi: 10.1111/j.1365-2958.1987.tb00523.x. [DOI] [PubMed] [Google Scholar]
  18. Nikaido H. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science. 1994 Apr 15;264(5157):382–388. doi: 10.1126/science.8153625. [DOI] [PubMed] [Google Scholar]
  19. Nikaido H., Rosenberg E. Y., Foulds J. Porin channels in Escherichia coli: studies with beta-lactams in intact cells. J Bacteriol. 1983 Jan;153(1):232–240. doi: 10.1128/jb.153.1.232-240.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nikaido H., Rosenberg E. Y. Porin channels in Escherichia coli: studies with liposomes reconstituted from purified proteins. J Bacteriol. 1983 Jan;153(1):241–252. doi: 10.1128/jb.153.1.241-252.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pangon B., Bizet C., Buré A., Pichon F., Philippon A., Regnier B., Gutmann L. In vivo selection of a cephamycin-resistant, porin-deficient mutant of Klebsiella pneumoniae producing a TEM-3 beta-lactamase. J Infect Dis. 1989 May;159(5):1005–1006. doi: 10.1093/infdis/159.5.1005. [DOI] [PubMed] [Google Scholar]
  23. Parr T. R., Jr, Moore R. A., Moore L. V., Hancock R. E. Role of porins in intrinsic antibiotic resistance of Pseudomonas cepacia. Antimicrob Agents Chemother. 1987 Jan;31(1):121–123. doi: 10.1128/aac.31.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Raimondi A., Traverso A., Nikaido H. Imipenem- and meropenem-resistant mutants of Enterobacter cloacae and Proteus rettgeri lack porins. Antimicrob Agents Chemother. 1991 Jun;35(6):1174–1180. doi: 10.1128/aac.35.6.1174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rice L. B., Carias L. L., Etter L., Shlaes D. M. Resistance to cefoperazone-sulbactam in Klebsiella pneumoniae: evidence for enhanced resistance resulting from the coexistence of two different resistance mechanisms. Antimicrob Agents Chemother. 1993 May;37(5):1061–1064. doi: 10.1128/aac.37.5.1061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Spratt B. G. Resistance to antibiotics mediated by target alterations. Science. 1994 Apr 15;264(5157):388–393. doi: 10.1126/science.8153626. [DOI] [PubMed] [Google Scholar]
  27. Yoshimura F., Nikaido H. Diffusion of beta-lactam antibiotics through the porin channels of Escherichia coli K-12. Antimicrob Agents Chemother. 1985 Jan;27(1):84–92. doi: 10.1128/aac.27.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. van de Klundert J. A., van Gestel M. H., Meerdink G., de Marie S. Emergence of bacterial resistance to cefamandole in vivo due to outer membrane protein deficiency. Eur J Clin Microbiol Infect Dis. 1988 Dec;7(6):776–778. doi: 10.1007/BF01975046. [DOI] [PubMed] [Google Scholar]

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