BIOCHEMISTRY. For the article “3β-Acetoxyandrost-1,5-diene-17-ethylene ketal functions as a potent antiandrogen with marginal agonist activity,” by Hiroshi Miyamoto, Padma Marwah, Ashok Marwah, Henry Lardy, and Chawnshang Chang, which appeared in issue 8, April 15, 2003, of Proc. Natl. Acad. Sci. USA (100, 4440–4444; First Published April 2, 2003, 10.1073/pnas.0831001100), in Fig. 1 A the hydroxyl group in position 17 for Δ5-androstenediol (Adiol), testosterone, and dihydrotestosterone (DHT) should be connected with a single, rather than a double, bond. The conclusions are unchanged by this typographical error. The corrected figure and its legend appear below.
Fig. 1.
The structures of DHEA derivatives and effects on AR transcriptional activity. (A) The structures of compounds nos. 5, 10, 14, 15, 16, and 17, DHEA, Adiol, testosterone, and DHT. (B) PC-3 cells were transfected with the WT AR expression plasmid pSG5-AR and MMTV-Luc. After transfection, cells were cultured for 24 h with 1 nM DHT or 1,000 nM of various DHEA derivatives. The Luc activity is presented relative to that of EtOH treatment (white bar; set as 1-fold). Values represent the mean ± SD of at least three determinations. (C) PC-3 cells were transfected with the pSG5-AR and MMTV-Luc. After transfection, cells were cultured for 24 h with various concentrations of compounds nos. 5, 10 (ADEK), 14, or 16 in the presence of 1 nM DHT. The Luc activity is presented relative to that in the presence of DHT (black bar; set as 100%). Values represent the mean ± SD of at least three determinations.
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