Abstract
Antibodies to human growth hormone (HGH) have been measured by radioimmunoassay on one or more occasions in 98 children with short stature treated with HGH, and in 51 other children. Positive antibody binding reactions occurred in nearly a third of these 149 children, but the great majority of these showed an assay curve significantly non-parallel to the standard, denoting the presence of only non-specific antibodies. However reactions above 0·1% antibody binding capacity in relation to the M.R.C. standard anti-serum are mostly specific to HGH.
Specific reaction over 0·1% occurred in 19 sera taken from 10 children, all of whom were under treatment with HGH. Only 4 of these children showed a slowing down of growth associated with the development of antibodies, 2 children permanently and 2 only for a period of three months with spontaneous recovery of growth rate and drop of antibodies. The level of antibodies which appears to cause growth inhibition is between 0·5% and 1% binding capacity of the M.R.C. standard.
In all, 4 out of 42 children with isolated growth hormone deficiency have developed `permanent' growth-retarding antibodies on HGH treatment. No children with other diagnoses have done so. Specific antibodies were not present in any of our patients before treatment.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
- FITSCHEN W. A QUANTITATIVE STUDY OF ANTIGEN--ANTIBODY COMBINATION DURING DISK ELECTROPHORESIS IN ACRYLAMIDE GEL USING IODINE-131 LABELLED HUMAN GROWTH HORMONE. Immunology. 1964 May;7:307–315. [PMC free article] [PubMed] [Google Scholar]
- Frasier S. D., Smith F. G., Jr Antibodies to human growth hormone. Development during therapy with human growth hormone. Am J Dis Child. 1966 Nov;112(5):383–388. doi: 10.1001/archpedi.1966.02090140055001. [DOI] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartree A. S. Separation and partial purification of the protein hormones from human pituitary glands. Biochem J. 1966 Sep;100(3):754–761. doi: 10.1042/bj1000754. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaplan S. L., Abrams C. A., Bell J. J., Conte F. A., Grumbach M. M. Growth and growth hormone. I. Changes in serum level of growth hormone following hypoglycemia in 134 children with growth retardation. Pediatr Res. 1968 Jan;2(1):43–63. doi: 10.1203/00006450-196801000-00005. [DOI] [PubMed] [Google Scholar]
- PARKER M. L., MARIZ I. K., DAUGHADAY W. H. RESISTANCE TO HUMAN GROWTH HORMONE IN PITUITARY DWARFISM: CLINICAL AND IMMUNOLOGIC STUDIES. J Clin Endocrinol Metab. 1964 Oct;24:997–1004. doi: 10.1210/jcem-24-10-997. [DOI] [PubMed] [Google Scholar]
- PRADER A., WAGNER H., SZEKY J., ILLIG R., TOUBER J. L., MAINGAY D. ACQUIRED RESISTANCE TO HUMAN GROWTH HORMONE CAUSED BY SPECIFIC ANTIBODIES. Lancet. 1964 Aug 22;2(7356):378–382. doi: 10.1016/s0140-6736(64)90390-3. [DOI] [PubMed] [Google Scholar]
- Prader A., Zachmann M., Poley J. R., Illig R., Széky J. Long-term treatment with human growth hormone (Raben) in small doses. Evaluation of 18 hypopituitary patients. Helv Paediatr Acta. 1967 Oct;22(5):423–440. [PubMed] [Google Scholar]
- ROTH J., GLICK S. M., YALOW R. S., BERSON S. A. ANTIBODIES TO HUMAN GROWTH HORMONE (HGH) IN HUMAN SUBJECTS TREATED WITH HGH. J Clin Invest. 1964 Jun;43:1056–1065. doi: 10.1172/JCI104989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seip M., Trygstad O. Experiences with human growth hormone in pituitary dwarfism. Acta Paediatr Scand. 1966 May;55(3):287–293. doi: 10.1111/j.1651-2227.1966.tb17656.x. [DOI] [PubMed] [Google Scholar]
- Tanner J. M., Whitehouse R. H. Growth response of 26 children with short stature given human growth hormone. Br Med J. 1967 Apr 8;2(5544):69–75. doi: 10.1136/bmj.2.5544.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tanner J. M., Whitehouse R. H. The effect of human growth hormone on subcutaneous fat thickness in hyposomatotrophic and panhypopituitary dwarfs. J Endocrinol. 1967 Oct;39(2):263–275. doi: 10.1677/joe.0.0390263. [DOI] [PubMed] [Google Scholar]