Abstract
Fluorimetry, paper and column chromatography, and bacterial inhibition assay have been compared in the quantitation of serum or blood phenylalanine at levels ranging from 1 to over 30 mg/100 ml. Special attention was paid to levels of 2 mg or less and to those in excess of 20 mg/100 ml, since these have therapeutic implications. Bacterial inhibition assay, under routine conditions, tended to read low or not at all at 2 mg or less, and to read high above 20 mg/100 ml so that it `failed safe' under such conditions. Paper chromatography was shown to be a simple, rapid, and economic method of getting accurate results at all levels in the range examined.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FARQUHAR J. W., KANSAS E. T., TAIT H. P. Problems of routine screening for phenylketonuria. Lancet. 1962 Sep 8;2(7254):498–500. doi: 10.1016/s0140-6736(62)90358-6. [DOI] [PubMed] [Google Scholar]
- WONG P. W., O'FLYNN M. E., INOUYE T. MICROMETHODS FOR MEASURING PHENYLALANINE AND TYROSINE IN SERUM. Clin Chem. 1964 Dec;10:1098–1104. [PubMed] [Google Scholar]
- WOOTTON I. D. P., KING E. J. Normal values for blood constituents; inter-hospital differences. Lancet. 1953 Mar 7;1(6758):470–471. doi: 10.1016/s0140-6736(53)91643-2. [DOI] [PubMed] [Google Scholar]