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. 1990 Jun 9;300(6738):1491–1494. doi: 10.1136/bmj.300.6738.1491

Human papillomaviruses in anogenital warts in children: typing by in situ hybridisation.

A F Padel 1, V A Venning 1, M F Evans 1, A M Quantrill 1, K A Fleming 1
PMCID: PMC1663223  PMID: 2164854

Abstract

OBJECTIVE--To identify the types of human papillomaviruses found in anogenital warts in children and to relate these to clinical and social information. DESIGN--In situ hybridisation using biotin labelled DNA probes to 11 types of human papillomavirus was performed on biopsy specimens from 17 children with anogenital warts. SETTING--Nuffield department of pathology and the department of dermatology, Oxford. PATIENTS--Children in one group were referred by general practitioners or paediatricians to the dermatology department, where biopsies were performed. The other children were seen in four different hospitals, and biopsy specimens were submitted to the laboratory at the physician's or pathologist's request. RESULTS--Of the 17 biopsy specimens, 10 contained cells positive with a probe to a genital human papillomavirus type (types 6 or 11), while six were positive with a skin virus type (types 2 or 3). One was negative. The virus type present bore no relation to the site or appearance of the warts. The virus type did, however, appear to correlate with groups of children. Skin types were commoner in older children (over 4 years), in those with a relative who had skin warts, and in children with warts elsewhere; there was no relation with the child's sex and no suspicion of sexual abuse in these children. These circumstances suggested non-sexual transmission, such as autoinoculation. In contrast, genital types were commoner in girls, in children under 3 years, in children with relatives with genital warts, and in those with no warts elsewhere. Nevertheless, there was suspicion or evidence of sexual abuse in only half these children, suggesting that other routes of transmission--for example, perinatal--might have been implicated. CONCLUSION--Anogenital warts in children may contain either skin or genital wart virus type. Although the type of human papillomavirus present may give some indication of the likely mode of transmission, this can be interpreted only in conjunction with all available clinical and social information. The type of virus does not provide proof of the presence or absence of sexual transmission.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Androphy E. J. Human papillomavirus. Current concepts. Arch Dermatol. 1989 May;125(5):683–685. [PubMed] [Google Scholar]
  2. Bender M. E. New concepts of condyloma acuminata in children. Arch Dermatol. 1986 Oct;122(10):1121–1124. [PubMed] [Google Scholar]
  3. Bennett R. S., Powell K. R. Human papillomaviruses: associations between laryngeal papillomas and genital warts. Pediatr Infect Dis J. 1987 Mar;6(3):229–232. doi: 10.1097/00006454-198703000-00001. [DOI] [PubMed] [Google Scholar]
  4. Burns J., Chan V. T., Jonasson J. A., Fleming K. A., Taylor S., McGee J. O. Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells. J Clin Pathol. 1985 Oct;38(10):1085–1092. doi: 10.1136/jcp.38.10.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burns J., Graham A. K., McGee J. O. Non-isotopic detection of in situ nucleic acid in cervix: an updated protocol. J Clin Pathol. 1988 Aug;41(8):897–899. doi: 10.1136/jcp.41.8.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burns J., Redfern D. R., Esiri M. M., McGee J. O. Human and viral gene detection in routine paraffin embedded tissue by in situ hybridisation with biotinylated probes: viral localisation in herpes encephalitis. J Clin Pathol. 1986 Oct;39(10):1066–1073. doi: 10.1136/jcp.39.10.1066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cohen S. R., Geller K. A., Seltzer S., Thompson J. W. Papilloma of the larynx and tracheobronchial tree in children. A retrospective study. Ann Otol Rhinol Laryngol. 1980 Nov-Dec;89(6 Pt 1):497–503. doi: 10.1177/000348948008900602. [DOI] [PubMed] [Google Scholar]
  8. Fleming K. A., Venning V., Evans M. DNA typing of genital warts and diagnosis of sexual abuse of children. Lancet. 1987 Aug 22;2(8556):454–454. doi: 10.1016/s0140-6736(87)90987-1. [DOI] [PubMed] [Google Scholar]
  9. Gissmann L., Boshart M., Dürst M., Ikenberg H., Wagner D., zur Hausen H. Presence of human papillomavirus in genital tumors. J Invest Dermatol. 1984 Jul;83(1 Suppl):26s–28s. doi: 10.1111/1523-1747.ep12281143. [DOI] [PubMed] [Google Scholar]
  10. Gissmann L., Pfister H., Zur Hausen H. Human papilloma viruses (HPV): characterization of four different isolates. Virology. 1977 Feb;76(2):569–580. doi: 10.1016/0042-6822(77)90239-2. [DOI] [PubMed] [Google Scholar]
  11. Gissmann L., Wolnik L., Ikenberg H., Koldovsky U., Schnürch H. G., zur Hausen H. Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proc Natl Acad Sci U S A. 1983 Jan;80(2):560–563. doi: 10.1073/pnas.80.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goldenring J. M. Condylomata acuminata in the evaluation of child sexual abuse. Arch Dermatol. 1987 Oct;123(10):1265–1266. [PubMed] [Google Scholar]
  13. Goldenring J. M. Re: Condylomata acuminata in the pediatric patient. J Urol. 1987 Nov;138(5):1259–1260. doi: 10.1016/s0022-5347(17)43577-4. [DOI] [PubMed] [Google Scholar]
  14. Gross G., Ikenberg H., Gissmann L., Hagedorn M. Papillomavirus infection of the anogenital region: correlation between histology, clinical picture, and virus type. Proposal of a new nomenclature. J Invest Dermatol. 1985 Aug;85(2):147–152. doi: 10.1111/1523-1747.ep12276576. [DOI] [PubMed] [Google Scholar]
  15. Krzyzek R. A., Watts S. L., Anderson D. L., Faras A. J., Pass F. Anogenital warts contain several distinct species of human papillomavirus. J Virol. 1980 Oct;36(1):236–244. doi: 10.1128/jvi.36.1.236-244.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oriel J. D. Anogenital papillomavirus infection in children. Br Med J (Clin Res Ed) 1988 May 28;296(6635):1484–1485. doi: 10.1136/bmj.296.6635.1484-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Orth G., Favre M., Croissant O. Characterization of a new type of human papillomavirus that causes skin warts. J Virol. 1977 Oct;24(1):108–120. doi: 10.1128/jvi.24.1.108-120.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Porter H. J., Khong T. Y., Evans M. F., Chan V. T., Fleming K. A. Parvovirus as a cause of hydrops fetalis: detection by in situ DNA hybridisation. J Clin Pathol. 1988 Apr;41(4):381–383. doi: 10.1136/jcp.41.4.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rock B., Naghashfar Z., Barnett N., Buscema J., Woodruff J. D., Shah K. Genital tract papillomavirus infection in children. Arch Dermatol. 1986 Oct;122(10):1129–1132. [PubMed] [Google Scholar]
  20. Schneider P. S., Krumholz B. A., Topp W. C., Steinberg B. M., Abramson A. L. Molecular heterogeneity of female genital wart (condylomata acuminata) papilloma viruses. Int J Gynecol Pathol. 1984;2(4):329–336. doi: 10.1097/00004347-198404000-00001. [DOI] [PubMed] [Google Scholar]
  21. Shelton T. B., Jerkins G. R., Noe H. N. Condylomata acuminata in the pediatric patient. J Urol. 1986 Mar;135(3):548–549. doi: 10.1016/s0022-5347(17)45732-6. [DOI] [PubMed] [Google Scholar]
  22. Vallejos H., Del Mistro A., Kleinhaus S., Braunstein J. D., Halwer M., Koss L. G. Characterization of human papilloma virus types in condylomata acuminata in children by in situ hybridization. Lab Invest. 1987 Jun;56(6):611–615. [PubMed] [Google Scholar]
  23. Wells M., Griffiths S., Lewis F., Bird C. C. Demonstration of human papillomavirus types in paraffin processed tissue from human ano-genital lesions by in-situ DNA hybridisation. J Pathol. 1987 Jun;152(2):77–82. doi: 10.1002/path.1711520203. [DOI] [PubMed] [Google Scholar]
  24. de Villiers E. M., Gissmann L., zur Hausen H. Molecular cloning of viral DNA from human genital warts. J Virol. 1981 Dec;40(3):932–935. doi: 10.1128/jvi.40.3.932-935.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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