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. 2005 Mar 15;563(Pt 3):975. doi: 10.1113/jphysiol.2005.563001

Errata

PMCID: PMC1665618

The paper by Hanley et al. (2005) contained a number of errors throughout the paper. These are corrected below.

On page 308, Synthesis and purification of β-oxidation intermediates, the components of Tris buffer solution were incorrectly listed. The first occurrence of EDTA should have appeared as MgCl2. The sentence should have read:

In brief, acyl-CoA synthetase (EC 6.2.1.3; Sigma) was used to synthesise 5-HD-CoA from 5-HD and CoASH in tris(hydroxymethyl)aminomethane (Tris) buffer solution containing (mm): 1.2 Na2ATP, 100 Tris, 9.1 MgCl2 and 1.8 EDTA (pH 7.5 with HCl).

On page 314, in the second complete sentence of the page, lauryl-carnitine was incorrectly written as lauryl-CoA. The sentence should have read:

This interpretation is supported by our finding that state-3 respiration induced by decanoyl-CoA or lauryl-carnitine was reduced by ≈40% after addition of 100 μm 5-HD-CoA (Fig. 7).

Two references, cited on pages 307 (Garlid et al. 1996) and 309 (Kawaguchi et al. 1981), were omitted from the list. The full references are given in the Reference list below.

References

  1. Garlid KD, Paucek P, Yarov-Yarovoy V, Sun X, Schindler PA. The mitochondrial KATP channel as a receptor for potassium channel openers. J Biol Chem. 1996;271:8796–8799. doi: 10.1074/jbc.271.15.8796. 10.1074/jbc.271.15.8796. [DOI] [PubMed] [Google Scholar]
  2. Hanley PJ, Dröse S, Brandt U, Lareau RA, Banerjee AL, Srivastava DK, Banaszak LJ, Barycki JJ, Van Veldhoven PP, Daut J. 5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for β-oxidation of fatty acids. J Physiol. 2005;562:307–318. doi: 10.1113/jphysiol.2004.073932. 10.1113/jphysiol.2004.073932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kawaguchi A, Yoshimura T, Okuda S. A new method for the preparation of acyl-CoA thioesters. J Biochem (Tokyo) 1981;89:337–339. doi: 10.1093/oxfordjournals.jbchem.a133207. [DOI] [PubMed] [Google Scholar]

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