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. 1971 Jul;42(3):428–436. doi: 10.1111/j.1476-5381.1971.tb07128.x

Binding of N-(2-bromo ethyl)-N-ethyl-N1-naphthylmethylamine HBr (SY28) to the proteins of guinea-pig vas deferens

J D P Graham, D R Mottram
PMCID: PMC1665661  PMID: 5560902

Abstract

1. Vasa deferentia from guinea-pigs were exposed to 14C-SY28 HBr (5·32 × 10-6M) washed, lipid extracted, and broken up.

2. The tissue was digested with papain, hydrolyzed with 6 N HCl, and gel filtration performed.

3. Aliquots at each stage were used for paper chromatography and autoradiography.

4. Three labelled spots (in addition to unbound 2-halogenoalkylamine) were located from acid hydrolysate and a fourth from gel filtrate.

5. Selected amino-acids were complexed with 14C-SY28 and the procedure repeated. The RF values were compared with those of the same amino-acids with and without 6 N HCl.

6. Histidine, aspartic acid, arginine and serine gave a significant degree of binding of 14C-SY28.

7. The theoretical implications are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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