Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1971 Dec;43(4):845–855. doi: 10.1111/j.1476-5381.1971.tb07221.x

Differences in the uptake, storage and metabolism of (+)- and (-)-noradrenaline

L L Iversen, B Jarrott, M A Simmonds
PMCID: PMC1665715  PMID: 5152031

Abstract

1. The rate of uptake of (+)- and (-)-noradrenaline was measured in isolated perfused hearts of reserpine treated rats, mice and guinea-pigs by fluorimetric analysis of the removal of catecholamine from the perfusion medium. In rat and mouse heart (-)-noradrenaline was taken up significantly more rapidly than (+)-noradrenaline, but no stereochemical specificity was found for noradrenaline uptake in guinea-pig hearts.

2. Using radioactively labelled (+)-14C-noradrenaline and (-)-3H-noradrenaline, the kinetic constants for uptake into noradrenaline-containing and dopamine-containing synaptosomes from rat brain were determined. The uptake by noradrenaline terminals in the hypothalamus had a higher affinity for (-)-noradrenaline than for the (+)-isomer, but no differences in affinity were found for uptake into dopamine terminals.

3. When equal amounts of labelled (+)- and (-)-noradrenaline were injected in vivo in double isotope experiments, the (+)-isomer disappeared more rapidly than the (-)-isomer from rat heart and spleen, but no significant differences were found between the rates of disappearance of the two isomers from rat brain or in the whole mouse.

4. Analysis of the radioactive metabolites of the two isomers of noradrenaline after administration of mixed doses of the labelled substances showed that a significantly higher proportion of (+)-noradrenaline was metabolized to normetanephrine than of (-)-noradrenaline, in the whole mouse, rat heart and rat brain.

Full text

PDF
845

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AXELROD J., TOMCHICK R. Enzymatic O-methylation of epinephrine and other catechols. J Biol Chem. 1958 Sep;233(3):702–705. [PubMed] [Google Scholar]
  2. Coyle J. T., Snyder S. H. Catecholamine uptake by synaptosomes in homogenates of rat brain: stereospecificity in different areas. J Pharmacol Exp Ther. 1969 Dec;170(2):221–231. [PubMed] [Google Scholar]
  3. Draskóczy P. R., Trendelenburg U. The uptake of l- and d-norepinephrine by the isolated perfused rabbit heart in relation to the stereospecificity of the sensitizing action of cocaine. J Pharmacol Exp Ther. 1968 Jan;159(1):66–73. [PubMed] [Google Scholar]
  4. Giachetti A., Shore P. A. Optical specificity of monoamine oxidase. Life Sci. 1966 Aug;5(15):1373–1378. doi: 10.1016/0024-3205(66)90112-3. [DOI] [PubMed] [Google Scholar]
  5. Giachetti A., Shore P. A. Studies in vitro of amine uptake mechanisms in heart. Biochem Pharmacol. 1966 May;15(5):607–614. doi: 10.1016/0006-2952(66)90028-1. [DOI] [PubMed] [Google Scholar]
  6. IVERSEN L. L. THE UPTAKE OF NORADRENALINE BY THE ISOLATED PERFUSED RAT HEART. Br J Pharmacol Chemother. 1963 Dec;21:523–537. doi: 10.1111/j.1476-5381.1963.tb02020.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. IVERSEN L. L., WHITBY L. G. Retention of injected catechol amines by the mouse. Br J Pharmacol Chemother. 1962 Oct;19:355–364. doi: 10.1111/j.1476-5381.1962.tb01199.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iversen L. L., Glowinski J., Axelrod J. The uptake and storage of H3-norepinephrine in the reserpine-pretreated rat heart. J Pharmacol Exp Ther. 1965 Nov;150(2):173–183. [PubMed] [Google Scholar]
  9. Iversen L. L., Glowinski J. Regional studies of catecholamines in the rat brain. II. Rate of turnover of catecholamines in various brain regions. J Neurochem. 1966 Aug;13(8):671–682. doi: 10.1111/j.1471-4159.1966.tb09874.x. [DOI] [PubMed] [Google Scholar]
  10. Jarrott B. Uptake and metabolism of catecholamines in the perfused hearts of different species. Br J Pharmacol. 1970 Apr;38(4):810–821. doi: 10.1111/j.1476-5381.1970.tb09890.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LINDMAR R., MUSCHOLL E. DIE WIRKUNG VON PHARMAKA AUF DIE ELIMINATION VON NORADRENALIN AUS DER PERFUSIONSFLUESSIGKEIT UND DIE NORADRENALINEUFNAHME IN DAS ISOLIERTE HERZ. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1964 Jul 28;247:469–492. doi: 10.1007/BF00329896. [DOI] [PubMed] [Google Scholar]
  12. MAICKEL R. P., BEAVEN M. A., BRODIE B. B. IMPLICATIONS OF UPTAKE AND STORAGE OF NOREPINEPHRINE BY SYMPATHETIC NERVE ENDINGS. Life Sci. 1963 Dec;12:953–958. doi: 10.1016/0024-3205(63)90064-x. [DOI] [PubMed] [Google Scholar]
  13. Snyder S. H., Coyle J. T. Regional differences in H3-norepinephrine and H3-dopamine uptake into rat brain homogenates. J Pharmacol Exp Ther. 1969 Jan;165(1):78–86. [PubMed] [Google Scholar]
  14. Stjärne L., von Euler U. S. Sterospecificity of amine uptake mechanism in nerve granules. J Pharmacol Exp Ther. 1965 Dec;150(3):335–340. [PubMed] [Google Scholar]
  15. von EULER U., LISHAJKO F. Improved technique for the fluorimetric estimation of catecholamines. Acta Physiol Scand. 1961 Apr;51:348–355. doi: 10.1111/j.1748-1716.1961.tb02128.x. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES