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. 1971 Sep;43(1):97–106. doi: 10.1111/j.1476-5381.1971.tb07160.x

Drug-induced changes in the release of [3H]-noradrenaline from field stimulated rat iris

L-O Farnebo, B Hamberger
PMCID: PMC1665932  PMID: 5136468

Abstract

1. Isolated rat irides were incubated with [3H]-noradrenaline [3H-NA] (10-7M), superfused with buffer and then stimulated by an electrical field. The effect of desipramine, clonidine, phentolamine, phenoxybenzamine, GD131, normetanephrine and 4-tropolone-acetamide on the stimulation-induced overflow of [3H]-NA was tested by adding the drug to the superfusing buffer. The effect of pretreatment with phentolamine or phenoxybenzamine on the stimulation-induced overflow of [3H]-NA was also studied.

2. The effect of desipramine, clonidine, phentolamine, phenoxybenzamine and GD131 on uptake of [3H]-NA in isolated irides was determined.

3. Desipramine moderately increased the stimulation-induced overflow at concentrations which almost completely inhibited neuronal uptake. It was calculated that in the isolated rat iris 30-40% of the released [3H]-NA is inactivated by reuptake into the nerve terminal. This figure may represent the true reuptake percentage in this preparation. Desipramine-induced inhibition of [3H]-NA release from the nerve terminal, possibly via a negative feed-back mechanism, may also contribute to this low figure.

4. Phentolamine and phenoxybenzamine, in concentrations or doses which did not inhibit neuronal uptake of [3H]-NA, consistently increased the stimulation-induced overflow. This increase was further augmented when neuronal uptake was inhibited.

5. The α-adrenoceptor stimulating drug clonidine decreased the stimulation-induced overflow.

6. GD131, normetanephrine and 4-tropolone-acetamide did not greatly affect the stimulation-induced overflow of [3H-NA].

7. It is concluded that the increased [3H]-NA overflow obtained after α-adrenoceptor blockade is due to an increased [3H]-NA release from the nerve terminals.

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Selected References

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