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. 1972 Dec;46(4):658–675. doi: 10.1111/j.1476-5381.1972.tb06891.x

Output of prostaglandins from the rabbit kidney, its increase on renal nerve stimulation and its inhibition by indomethacin

Heather A Davis, E W Horton
PMCID: PMC1666365  PMID: 4655266

Abstract

1. In each of six experiments, prostaglandins were identified in the renal venous blood of the rabbit. The concentrations in renal venous blood were up to 45 times higher than in aortic blood, suggesting that most of the prostaglandins originate from the kidney.

2. Prostaglandins E2, F2a and a prostaglandin of the A, C or B series were estimated by biological assay after solvent partition and column or thin-layer chromatography.

3. Prostaglandins E2 and F2a were identified conclusively by combined gas chromatography-mass spectrometry.

4. Electrical stimulation of the renal nerve increased the output of prostaglandins.

5. Indomethacin (10 mg/kg) injected intravenously, reduced the output of prostaglandins into renal venous blood and prevented the increase in output on renal nerve stimulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carrière S., Friborg J., Guay J. P. Vasodilators, intrarenal blood flow, and natriuresis in the dog. Am J Physiol. 1971 Jul;221(1):92–98. doi: 10.1152/ajplegacy.1971.221.1.92. [DOI] [PubMed] [Google Scholar]
  2. Daniels E. G., Hinman J. W., Leach B. E., Muirhead E. E. Identification of prostaglandin E2 as the principal vasodepressor lipid of rabbit renal medulla. Nature. 1967 Sep 16;215(5107):1298–1299. doi: 10.1038/2151298a0. [DOI] [PubMed] [Google Scholar]
  3. Davis H. A., Horton E. W., Jones K. B., Quilliam J. P. Identification of prostaglandins in prevertebral venous blood after preganglionic stimulation of the cat superior cervical ganglion. Br J Pharmacol. 1971 Aug;42(4):569–583. doi: 10.1111/j.1476-5381.1971.tb07141.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ferreira S. H., Moncada S., Vane J. R. Indomethacin and aspirin abolish prostaglandin release from the spleen. Nat New Biol. 1971 Jun 23;231(25):237–239. doi: 10.1038/newbio231237a0. [DOI] [PubMed] [Google Scholar]
  5. Hedqvist P. Prostaglandin E compounds and sympathetic neuromuscular transmission. Ann N Y Acad Sci. 1971 Apr 30;180:410–415. doi: 10.1111/j.1749-6632.1971.tb53208.x. [DOI] [PubMed] [Google Scholar]
  6. Horton E. W., Jones R. L. Prostaglandins A1,A2 and 19-hydroxy A1; their actions on smooth muscle and their inactivation on passage through the pulmonary and hepatic portal vascular beds. Br J Pharmacol. 1969 Nov;37(3):705–722. doi: 10.1111/j.1476-5381.1969.tb08509.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johansson B., Sparks H., Biber B. The escape of the renal blood flow response during sympathetic nerve stimulation. Angiologica. 1970;7(6):333–343. doi: 10.1159/000157848. [DOI] [PubMed] [Google Scholar]
  8. Jones R. L. 15-hydroxy-9-oxoprosta-11, 13-dienoic acid as the product of a prostaglandin isomerase. J Lipid Res. 1972 Jul;13(4):511–518. [PubMed] [Google Scholar]
  9. Jones R. L. A prostaglandin isomerase in cat plasma. Biochem J. 1970 Oct;119(5):64P–65P. doi: 10.1042/bj1190064pb. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LEE J. B., COVINO B. G., TAKMAN B. H., SMITH E. R. RENOMEDULLARY VASODEPRESSOR SUBSTANCE, MEDULLIN: ISOLATION, CHEMICAL CHARACTERIZATION AND PHYSIOLOGICAL PROPERTIES. Circ Res. 1965 Jul;17:57–77. doi: 10.1161/01.res.17.1.57. [DOI] [PubMed] [Google Scholar]
  11. Lee J. B., Crowshaw K., Takman B. H., Attrep K. A. The identification of prostaglandins E(2), F(2alpha) and A(2) from rabbit kidney medulla. Biochem J. 1967 Dec;105(3):1251–1260. doi: 10.1042/bj1051251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lee J., Kanneqiesser H., O'Toole J., Westura E. Hypertension and the renomedullary prostaglandins: a human study of the antihypertensive effects of PGA 1 . Ann N Y Acad Sci. 1971 Apr 30;180:218–240. doi: 10.1111/j.1749-6632.1971.tb53194.x. [DOI] [PubMed] [Google Scholar]
  13. McGiff J. C., Crowshaw K., Terragno N. A., Lonigro A. J. Renal prostaglandins: possible regulators of the renal actions of pressor hormones. Nature. 1970 Sep 19;227(5264):1255–1257. doi: 10.1038/2271255b0. [DOI] [PubMed] [Google Scholar]
  14. McGiff J. C., Crowshaw K., Terragno N. A., Malik K. U., Lonigro A. J. Differential effect of noradrenaline and renal nerve stimulation on vascular resistance in the dog kidney and the release of a prostaglandin E-like substance. Clin Sci. 1972 Feb;42(2):223–233. doi: 10.1042/cs0420223. [DOI] [PubMed] [Google Scholar]
  15. McGiff J. C., Terragno N. A., Strand J. C., Lee J. B., Lonigro A. J., Ng K. K. Selective passage of prostaglandins across the lung. Nature. 1969 Aug 16;223(5207):742–745. doi: 10.1038/223742b0. [DOI] [PubMed] [Google Scholar]
  16. Muirhead E. E., Brooks B., Pitcock J. A., Stephenson P. Renomedullary antihypertensive function in accelerated (malignant) hypertension. Observations on renomedullary interstitial cells. J Clin Invest. 1972 Jan;51(1):181–190. doi: 10.1172/JCI106790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Piper P., Vane J. The release of prostaglandins from lung and other tissues. Ann N Y Acad Sci. 1971 Apr 30;180:363–385. doi: 10.1111/j.1749-6632.1971.tb53205.x. [DOI] [PubMed] [Google Scholar]
  18. Pomeranz B. H., Birtch A. G., Barger A. C. Neural contrfl of intrarenal blood flow. Am J Physiol. 1968 Nov;215(5):1067–1081. doi: 10.1152/ajplegacy.1968.215.5.1067. [DOI] [PubMed] [Google Scholar]
  19. Poyser N. L. Production of prostaglandins by the guinea-pig uterus. J Endocrinol. 1972 Jul;54(1):147–159. doi: 10.1677/joe.0.0540147. [DOI] [PubMed] [Google Scholar]
  20. Smith J. B., Willis A. L. Aspirin selectively inhibits prostaglandin production in human platelets. Nat New Biol. 1971 Jun 23;231(25):235–237. doi: 10.1038/newbio231235a0. [DOI] [PubMed] [Google Scholar]
  21. Smith W. L., Lands W. E. Stimulation and blockade of prostaglandin biosynthesis. J Biol Chem. 1971 Nov;246(21):6700–6702. [PubMed] [Google Scholar]
  22. Stjärne L., Hedqvist P., Bygdeman S. Neurotransmitter quantum released from sympathetic nerves in cat's skeletal muscle. Life Sci. 1969 Feb 1;8(3):189–196. doi: 10.1016/0024-3205(69)90093-9. [DOI] [PubMed] [Google Scholar]
  23. Thompson C. J., Los M., Horton E. W. The separation, identification and estimation of prostaglandins in nanogram quantities by combined gas chromatography-mass spectrometry. Life Sci I. 1970 Sep 1;9(17):983–988. doi: 10.1016/0024-3205(70)90121-9. [DOI] [PubMed] [Google Scholar]
  24. Vane J. R. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol. 1971 Jun 23;231(25):232–235. doi: 10.1038/newbio231232a0. [DOI] [PubMed] [Google Scholar]

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