Abstract
1. Reserpine administration resulted in a larger initial decline in adrenal catecholamines in spontaneously hypertensive rats (SHR) than in normotensive Wistar rats (NWR); the difference was eliminated by pretreatment with cholisdondamine. 2. Reserpine also produced a larger increase in SHR catecholamines and dopamine beta-hydroxylase several days later; chlorisondamine pretreatment did not prevent the increases, although it did slightly slow the increases. 3. Vesicles from SHR, or NWR incubated with reserpine in vitro demonstrated equivalent inhibition of adenosine 5'-triphosphate (ATP)-Mg-2+-stimulated adrenaline uptake. 4. Recovery of uptake was more rapid in SHR than in NWR after reserpine inhibition, and this was associated with a burst of new vesicle synthesis in the SHR; chlorisondamine pretreatment reduced the number of new, immature vesicles in reserpine-treated SHR. 5. Both SHR and NWR secreted equal proportions of their adrenal catecholamine contents after nicotine administration. 6. These data suggest that the sympatho-adrenal system of the SHR exhibits an enhanced reflex response to reserpine but that reserpine is equally effective in SHR and NWR in producing blockade of vesicular catecholamine transport; these alterations can affect markedly the actions of autonomic drugs in the SHR.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Friedman S., Kaufman S. 3,4-dihydroxyphenylethylamine beta-hydroxylase. Physical properties, copper content, and role of copper in the catalytic acttivity. J Biol Chem. 1965 Dec;240(12):4763–4773. [PubMed] [Google Scholar]
- Green H. O., Slotkin T. A. Reserpine-like effects of harmine on isolated adrenal medullary vesicles. Mol Pharmacol. 1973 Nov;9(6):748–755. [PubMed] [Google Scholar]
- JONASSON J., ROSENGREN E., WALDECK B. EFFECTS OF SOME PHARMACOLOGICALLY ACTIVE AMINES ON THE UPTAKE OF ARYLALKYLAMINES BY ADRENAL MEDULLARY GRANULES. Acta Physiol Scand. 1964 Jan-Feb;60:136–140. doi: 10.1111/j.1748-1716.1964.tb02876.x. [DOI] [PubMed] [Google Scholar]
- Louis W. J., Spector S., Tabei R., Sjoerdsma A. Synthesis and turnover of norepinephrine in the heart of the spontaneously hypertensive rat. Circ Res. 1969 Jan;24(1):85–91. doi: 10.1161/01.res.24.1.85. [DOI] [PubMed] [Google Scholar]
- Louis W. J., Tabei R., Spector S., Sjoerdsma A. Studies on the spontaneously hypertensive rat. Genealogy, effects of varying salt intake, and kinetics of catecholamine metabolism. Circ Res. 1969 May;24(5 Suppl):93–102. [PubMed] [Google Scholar]
- MERRILLS R. J. A SEMIAUTOMATIC METHOD FOR DETERMINATION OF CATECHOLAMINES. Anal Biochem. 1963 Sep;6:272–282. doi: 10.1016/0003-2697(63)90135-0. [DOI] [PubMed] [Google Scholar]
- Slotkin T. A., Ferris R. M., Kirshner N. Compartmental analysis of amine storage in bovine adrenal medullary granules. Mol Pharmacol. 1971 May;7(3):308–316. [PubMed] [Google Scholar]
- Slotkin T. A., Green H. O. Adrenal medullary storage vesicles of the spontaneously hypertensive rat. Biochem Pharmacol. 1975 Jan 15;24(2):173–180. doi: 10.1016/0006-2952(75)90274-9. [DOI] [PubMed] [Google Scholar]
- Slotkin T. A., Kirshner N. All-or-none secretion of adrenal medullary storage vesicle contents in the rat. Biochem Pharmacol. 1973 Jan 15;22(2):205–219. doi: 10.1016/0006-2952(73)90274-8. [DOI] [PubMed] [Google Scholar]
- Slotkin T. A., Kirshner N. Recovery of rat adrenal amine stores after insulin administration. Mol Pharmacol. 1973 Jan;9(1):105–116. [PubMed] [Google Scholar]
- Slotkin T. A., Kirshner N. Uptake, storage, and distribution of amines in bovine adrenal medullary vesicles. Mol Pharmacol. 1971 Nov;7(6):581–592. [PubMed] [Google Scholar]
- Slotkin T. A. Maturation of the adrenal medulla. I. Uptake and storage of amines in isolated storage vesicles of the rat. Biochem Pharmacol. 1973 Aug 15;22(16):2023–2032. doi: 10.1016/0006-2952(73)90083-x. [DOI] [PubMed] [Google Scholar]