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. 1975 Nov;55(3):387–392. doi: 10.1111/j.1476-5381.1975.tb06942.x

Effects of fasting, stress and drugs on gastric glycoprotein synthesis in the rat.

J B Dekanski, A Macdonald, P Sacra
PMCID: PMC1666692  PMID: 1203624

Abstract

1 The relationship between gastric mucosal damage and synthesis of gastric glycoproteins, as measured by the rate of incorporation of N-acetyl-[3H]glucosamine, was investigated in rats after fasting and restraint stress and a single administration of aspirin (200 mg/kg, orally), phenylbutazone (200 mg/kg, orally), prednisolone (200 mg/kg, orally), or adrenaline (2 mg/kg, i.p.). In one experiment, the effects of aspirin and phenylbutazone on carbohydrate content of the glycoproteins were also determined. 2 Restraint stress, phenylbutazone and aspirin resulted in acute gastric mucosal erosions in some of the rats. Adrenaline produced severe sub-mucosal haemorrhage, but no erosions or ulceration, while prednisolone and fasting gave no gross pathology. 3 The rate of incorporation of N-acetyl-[3H]glucosamine into glycoproteins was decreased after all treatments except adrenaline. In the groups receiving restraint stress, aspirin or phenylbutazone, the decreases were more marked in rats which developed erosions than in those with no gastric pathology. 4 Aspirin and phenylbutazone also produced changes in the carbohydrate content of the glycoproteins, the effects again being greater in the rats which developed erosions. 5 The results are discussed in the context of a possible association between erosion formation and glycoprotein synthesis and it is proposed that inhibition of mucus glycoprotein biosynthesis may be one mode of action of stress and drugs in causing gastric mucosal damage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  2. BRODIE D. A., HANSON H. M. A study of the factors involved in the production of gastric ulcers by the restraint technique. Gastroenterology. 1960 Mar;38:353–360. [PubMed] [Google Scholar]
  3. CODE C. F., HIGGINS J. A., MOLL J. C., ORVIS A. L., SCHOLER J. F. The influence of acid on the gastric absorption of water, sodium and potassium. J Physiol. 1963 Apr;166:110–119. doi: 10.1113/jphysiol.1963.sp007093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAVENPORT H. W. GASTRIC MUCOSAL INJURY BY FATTY AND ACETYLSALICYLIC ACIDS. Gastroenterology. 1964 Mar;46:245–253. [PubMed] [Google Scholar]
  5. DAVENPORT H. W., WARNER H. A., CODE C. F. FUNCTIONAL SIGNIFICANCE OF GASTRIC MUCOSAL BARRIER TO SODIUM. Gastroenterology. 1964 Aug;47:142–152. [PubMed] [Google Scholar]
  6. Dekanski J. B., Macdonald A., Parke D. V. Proceedings: The effect of mucosal damage on gastric glycoprotein synthesis in the rat. Br J Pharmacol. 1974 Nov;52(3):464P–465P. [PMC free article] [PubMed] [Google Scholar]
  7. Goodier T. E., Horwich L., Galloway R. W. Morphological observations on gastric ulcers treated with carbenoxolone sodium. Gut. 1967 Dec;8(6):544–547. doi: 10.1136/gut.8.6.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kirsner J. B. Peptic ulcer--a review of the literature for 1965. Gastroenterology. 1966 Sep;51(3):403–429. [PubMed] [Google Scholar]
  9. Ludwig W. M., Lipkin M. Biochemical and cytological alterations in gastric mucosa of guinea pigs under restraint stress. Gastroenterology. 1969 May;56(5):895–902. [PubMed] [Google Scholar]
  10. Lukie B. E., Forstner G. G. Synthesis of intestinal glycoprotein. Incorporation of (I- 14 C) glucosamine in vitro. Biochim Biophys Acta. 1971 Feb 28;261(2):353–364. [PubMed] [Google Scholar]
  11. MENGUY R., MASTERS Y. F. EFFECTS OF ASPIRIN ON GASTRIC MUCOUS SECRETION. Surg Gynecol Obstet. 1965 Jan;120:92–98. [PubMed] [Google Scholar]
  12. Menguy R., Desbaillets L. Influence of phenylbutazone on gastric secretion of mucus. Proc Soc Exp Biol Med. 1967 Aug-Sep;125(4):1108–1111. doi: 10.3181/00379727-125-32288. [DOI] [PubMed] [Google Scholar]
  13. ROBERT A., BAYER R. B., NEZAMIS J. E. GASTRIC MUCUS CONTENT DURING DEVELOPMENT OF ULCERS IN FASTING RATS. Gastroenterology. 1963 Dec;45:740–751. [PubMed] [Google Scholar]
  14. ROBERT A., NEZAMIS J. E. EFFECT OF PREDNISOLONE ON GASTRIC MUCUS CONTENT AND ON ULCER FORMATION. Proc Soc Exp Biol Med. 1963 Dec;114:545–550. doi: 10.3181/00379727-114-28728. [DOI] [PubMed] [Google Scholar]
  15. Sircus W. Carbenoxolone sodium. Gut. 1972 Oct;13(10):816–824. doi: 10.1136/gut.13.10.816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spiro R. G. Glycoproteins. Annu Rev Biochem. 1970;39:599–638. doi: 10.1146/annurev.bi.39.070170.003123. [DOI] [PubMed] [Google Scholar]
  17. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]

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