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. 1971 May;42(1):43–55. doi: 10.1111/j.1476-5381.1971.tb07085.x

Differentiation between the actions of acetylcholine and tetramethylammonium on the isolated taenia of the guinea-pig caecum by hemicholinium-3

F Mitchelson
PMCID: PMC1666985  PMID: 4325608

Abstract

1. Contractions of the isolated taenia of the guinea-pig caecum produced by acetylcholine and TMA were examined in the presence of various antagonists and anticholinesterases.

2. Hemicholinium-3 (HC-3) (50-400 μg/ml) inhibited contractions or relaxations produced by TMA but not contractions produced by acetylcholine. The inhibition was rapid in onset and readily reversible. Contractions produced by transmural stimulation were unaffected by HC-3 but responses produced by nicotine were inhibited.

3. Low concentrations of hyoscine and benzhexol inhibited responses to acetylcholine to a greater extent than those to TMA.

4. Morphine, raised concentrations of Mg++ or reduced concentrations of Ca++ inhibited contractions produced by TMA and by acetylcholine to a similar extent.

5. Edrophonium, in concentrations which preferentially inhibit acetylcholinesterase, increased contractions produced by acetylcholine and converted responses to nicotine or transmural stimulation into contractions or biphasic responses with a marked contraction phase but did not increase contractions produced by TMA.

6. Iso-OMPA, in concentrations which preferentially inhibit butyrylcholinesterase, had no effect on responses to acetylcholine, nicotine, transmural stimulation or TMA.

7. HC-3 inhibited contractions produced by TMA in the presence of anticholinesterases but had little effect on contractions produced by acetylcholine.

8. These results suggest that TMA produces contractions by acting directly on receptors of the smooth muscle. An analysis of possible reasons for HC-3 (in the concentrations used) acting as an antagonist of TMA but not of acetylcholine indicates that the findings do not necessarily contradict the interpretation that both agonists act on the same receptor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALDRIDGE W. N. The differentiation of true and pseudo cholinesterase by organophosphorus compounds. Biochem J. 1953 Jan;53(1):62–67. doi: 10.1042/bj0530062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akubue P. I. The site of action of drugs on the isolated taenia caeci from the guinea-pig. Br J Pharmacol Chemother. 1966 Aug;27(2):347–365. doi: 10.1111/j.1476-5381.1966.tb01667.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BURN J. H., RAND M. J. The relation of circulating noradrenaline to the effect of sympathetic stimulation. J Physiol. 1960 Feb;150:295–305. doi: 10.1113/jphysiol.1960.sp006388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bebbington A., Brimblecombe R. W., Rowsell D. G. The interaction of muscarinic drugs with the postganglionic acetylcholine receptor. Br J Pharmacol Chemother. 1966 Jan;26(1):68–78. doi: 10.1111/j.1476-5381.1966.tb01812.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bennett M. R., Burnstock G., Holman M. Transmission from intramural inhibitory nerves to the smooth muscle of the guinea-pig taenia coli. J Physiol. 1966 Feb;182(3):541–558. doi: 10.1113/jphysiol.1966.sp007836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bertolini A., Greggia A., Ferrari W. Atropine-like properties of hemicholinium-3. Life Sci. 1967 Mar 1;6(5):537–543. doi: 10.1016/0024-3205(67)90057-4. [DOI] [PubMed] [Google Scholar]
  7. György L., Pfeifer A. K., Kenyeres J. The interaction of hemicholinium-3 and oxotremorine in isolated organ preparations. J Pharm Pharmacol. 1970 Feb;22(2):96–100. doi: 10.1111/j.2042-7158.1970.tb08399.x. [DOI] [PubMed] [Google Scholar]
  8. Hobbiger F., Mitchelson F., Rand M. J. The actions of some cholinomimetic drugs on the isolated taenia of the guinea-pig caecum. Br J Pharmacol. 1969 May;36(1):53–69. doi: 10.1111/j.1476-5381.1969.tb08303.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KOELLE G. B. A new general concept of the neurohumoral functions of acetylcholine and acetylcholinesterase. J Pharm Pharmacol. 1962 Feb;14:65–90. doi: 10.1111/j.2042-7158.1962.tb11057.x. [DOI] [PubMed] [Google Scholar]
  10. Leaders F. E., Pan P. J. Local cholinergic-adrenergic interaction in the dog salivary gland preparation. Arch Int Pharmacodyn Ther. 1967 Jan;165(1):71–80. [PubMed] [Google Scholar]
  11. MCEWEN L. M. The effect on the isolated rabbit heart of vagal stimulation and its modification by cocaine, hexamethonium and ouabain. J Physiol. 1956 Mar 28;131(3):678–689. doi: 10.1113/jphysiol.1956.sp005493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MCKINSTRY D. N., KOENIG E., KOELLE W. A., KOELLE G. B. THE RELEASE OF ACETYLCHOLINE FROM A SYMPATHETIC GANGLION BY CARBACHOL. RELATIONSHIP TO THE FUNCTIONAL SIGNIFICANCE OF THE LOCALIZATION OF ACETYLCHOLINESTERASE. Can J Biochem Physiol. 1963 Dec;41:2599–2609. [PubMed] [Google Scholar]
  13. McKinstry D. N., Koelle G. B. Acetylcholine release from the cat superior cervical ganglion by carbachol. J Pharmacol Exp Ther. 1967 Aug;157(2):319–327. [PubMed] [Google Scholar]
  14. RAND M. J., RIDEHALGH A. ACTIONS OF HEMICHOLINIUM AND TRIETHYLCHOLINE ON RESPONSES OF GUINEA-PIG COLON TO STIMULATION OF AUTONOMIC NERVES. J Pharm Pharmacol. 1965 Mar;17:144–156. doi: 10.1111/j.2042-7158.1965.tb07633.x. [DOI] [PubMed] [Google Scholar]
  15. VOLLE R. L., KOELLE G. B. The physiological role of acetyl-cholinesterase (AChE) in sympathetic ganglia. J Pharmacol Exp Ther. 1961 Aug;133:223–240. [PubMed] [Google Scholar]

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