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. 1976 May;57(1):3–15. doi: 10.1111/j.1476-5381.1976.tb07650.x

Effects of amino acids and convulsants on spontaneous action potentials in cerebellar cortex slices.

K Okamoto, J H Quastel
PMCID: PMC1667019  PMID: 1276540

Abstract

1 Picrotoxin selectively and reversibly suppressed the inhibitory action of gamma-aminobutyric acid (GABA), but not that of glycine, taurine or beta-alanine, on the frequency of spontaneous spike discharges in guinea-pig cerebellar slices. Strychnine reversibly suppressed the inhibitory action of glycine, taurine or beta-alanine but had no effect on that of GABA. 2 GABA, glycine, taurine and beta-alanine showed an early excitatory effect that was unaffected by picrotoxin or strychnine. 3 Studies of the dose-response relations indicated a competition between the amino acid and the convulsant at a common receptor site. 4 Kinetic analyses of the dose-response relations for the amino acids in the presence or absence of picrotoxin or strychnine indicated that the number of molecules of amino acid combining with the receptor site in order to produce a response (inhibition or excitation) was 3 for GABA, 2 for glycine, 3 for taurine and 4 for beta-alanine. There appeared to be no evidence that the response was due to the cooperativity between the amino acid receptor complexes. The number of molecules of convulsant that combined with the receptor site was 1 for either strychnine or picrotoxin. 5 Mixtures of glycine with taurine or beta-alanine, in contrast to those with GABA, appeared not to give additive inhibitory effects.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cooke J. D., Okamoto K., Quastel D. M. The role of calcium in depolarization-secretion coupling at the motor nerve terminal. J Physiol. 1973 Jan;228(2):459–497. doi: 10.1113/jphysiol.1973.sp010095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cooke J. D., Quastel D. M. Transmitter release by mammalian motor nerve terminals in response to focal polarization. J Physiol. 1973 Jan;228(2):377–405. doi: 10.1113/jphysiol.1973.sp010092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Curtis D. R., Duggan A. W., Felix D., Johnston G. A. Bicuculline, an antagonist of GABA and synaptic inhibition in the spinal cord of the cat. Brain Res. 1971 Sep 10;32(1):69–96. doi: 10.1016/0006-8993(71)90156-9. [DOI] [PubMed] [Google Scholar]
  4. Curtis D. R., Duggan A. W., Felix D., Johnston G. A. GABA, bicuculline and central inhibition. Nature. 1970 Jun 27;226(5252):1222–1224. doi: 10.1038/2261222a0. [DOI] [PubMed] [Google Scholar]
  5. Curtis D. R., Duggan A. W., Felix D., Johnston G. A., McLennan H. Antagonism between bicuculline and GABA in the cat brain. Brain Res. 1971 Oct 8;33(1):57–73. doi: 10.1016/0006-8993(71)90305-2. [DOI] [PubMed] [Google Scholar]
  6. Curtis D. R., Duggan A. W., Johnston G. A. The specificity of strychnine as a glycine antagonist in the mammalian spinal cord. Exp Brain Res. 1971 Jun 29;12(5):547–565. doi: 10.1007/BF00234248. [DOI] [PubMed] [Google Scholar]
  7. Curtis D. R. The pharmacology of spinal postsynaptic inhibition. Prog Brain Res. 1969;31:171–189. doi: 10.1016/s0079-6123(08)63237-9. [DOI] [PubMed] [Google Scholar]
  8. Davidoff R. A., Aprison M. H., Werman R. The effects of strychnine on the inhibition of interneurons by glycine and gamma-aminobutyric acid. Int J Neuropharmacol. 1969 Mar;8(2):191–194. doi: 10.1016/0028-3908(69)90013-6. [DOI] [PubMed] [Google Scholar]
  9. Feltz A. Competitive interaction of beta-guanidino propionic acid and gamma-aminobutyric acid on the muscle fibre of the crayfish. J Physiol. 1971 Jul;216(2):391–401. doi: 10.1113/jphysiol.1971.sp009531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Freeman A. R. Electrophysiological analysis of the actions of strychnine, bicuculline and picrotoxin on the axonal membrane. J Neurobiol. 1973;4(6):567–582. doi: 10.1002/neu.480040609. [DOI] [PubMed] [Google Scholar]
  11. Gahwiler B. H., Mamoon A. M., Schlapfer W. T., Tobias C. A. Effects of temperature on spontaneous bioelectric activity of cultured nerve cells. Brain Res. 1972 May 26;40(2):527–533. doi: 10.1016/0006-8993(72)90157-6. [DOI] [PubMed] [Google Scholar]
  12. Geller H. M., Woodward D. J. Responses of cultured cerebellar neurons to iontophoretically applied amino acids. Brain Res. 1974 Jul 5;74(1):67–80. doi: 10.1016/0006-8993(74)90112-7. [DOI] [PubMed] [Google Scholar]
  13. HILD W., TASAKI I. Morphological and physiological properties of neurons and glial cells in tissue culture. J Neurophysiol. 1962 Mar;25:277–304. doi: 10.1152/jn.1962.25.2.277. [DOI] [PubMed] [Google Scholar]
  14. Hackett J. T. Electrophysiological properties of neuronal circuits in the frog cerebellum in vitro. Brain Res. 1972 Dec 24;48:385–389. doi: 10.1016/0006-8993(72)90195-3. [DOI] [PubMed] [Google Scholar]
  15. Hill R. G., Simmonds M. A. A method for comparing the potencies of -aminobutyric acid antagonists on single cortical neurones using micro-iontophoretic techniques. Br J Pharmacol. 1973 May;48(1):1–11. doi: 10.1111/j.1476-5381.1973.tb08216.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hill R. G., Simmonds M. A., Straughan D. W. A comparative study of some convulsant substances as gamma-aminobutyric acid antagonists in the feline cerebral cortex. Br J Pharmacol. 1973 Sep;49(1):37–51. doi: 10.1111/j.1476-5381.1973.tb08266.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kawamura H., Provini L. Depression of cerebellar Purkinje cells by microiontophoretic application of GABA and related amino acids. Brain Res. 1970 Dec 1;24(2):293–304. doi: 10.1016/0006-8993(70)90108-3. [DOI] [PubMed] [Google Scholar]
  18. MARTIN A. R. A further study of the statistical composition on the end-plate potential. J Physiol. 1955 Oct 28;130(1):114–122. doi: 10.1113/jphysiol.1955.sp005397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Okamoto K., Quastel J. H. Spontaneous action potentials in isolated guinea-pig cerebellar slices: effects of amino acids and conditions affecting sodium and water uptake. Proc R Soc Lond B Biol Sci. 1973 Aug 31;184(1074):83–90. doi: 10.1098/rspb.1973.0032. [DOI] [PubMed] [Google Scholar]
  20. Takeuchi A., Takeuchi N. A study of the action of picrotoxin on the inhibitory neuromuscular junction of the crayfish. J Physiol. 1969 Nov;205(2):377–391. doi: 10.1113/jphysiol.1969.sp008972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. VAN MAANEN E. F. The antagonism between acetylcholine and the curare alkaloids, d-tubocurarine, c-curarine-I, c-toxiferine-II and beta-erythroidine in the rectus abdominis of the frog. J Pharmacol Exp Ther. 1950 Jun;99(2):255–264. [PubMed] [Google Scholar]
  22. Werman R. An electrophysiological approach to drug-receptor mechanisms. Comp Biochem Physiol. 1969 Sep 15;30(6):997–1017. doi: 10.1016/0010-406x(69)91038-x. [DOI] [PubMed] [Google Scholar]
  23. Woodward D. J., Hoffer B. J., Siggins G. R., Oliver A. P. Inhibition of Purkinje cells in the frog cerebellum. II. Evidence for GABA as the inhibitory transmitter. Brain Res. 1971 Oct 8;33(1):91–100. doi: 10.1016/0006-8993(71)90308-8. [DOI] [PubMed] [Google Scholar]
  24. Yamamoto C. Electrical activity observed in vitro in thin sections from guinea-pig cerebellum. Jpn J Physiol. 1974 Apr;24(2):177–188. doi: 10.2170/jjphysiol.24.177. [DOI] [PubMed] [Google Scholar]

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