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. 1976 Sep;58(1):9–16. doi: 10.1111/j.1476-5381.1976.tb07687.x

Effects of drugs affecting endogenous amines or cyclic nucleotides on ethanol withdrawal head twitches in mice.

H O Collier, M D Hammond, C Schneider
PMCID: PMC1667129  PMID: 987821

Abstract

1 Twenty-four hours after ethanol withdrawal, dependent mice exhibited frequent head twitching. Naive mice exhibited similar twitching 15 min after treatment with 5-hydroxytryptophan (5-HTP) or 6 h after alpha-methyl-p-tyrosine (AMPT). Ethanol lessened the incidence of head twitches induced by any of these treatments. 5-HTP and AMPT each increased the incidence of head twitches induced by withdrawal of ethanol from dependent mice. 2 Drugs that affect the amount or activity of endogenous amines or cyclic nucleotides modified the incidence of head twitches. Nearly all drugs acted in the same direction on twitching elicited by any of these three treatments. 3 The incidence was lessened by: (a) methysergide, methergoline, MA 1420, p-chlorophenylalanine and p-chloroamphetamine; (b) dopamine, noradrenaline, L-DOPA, amphetamine and apomorphine; (c) hyoscine and nicotine; and (d) adenosine triphosphate, dibutyryl cyclic adenosine-3',5'-monophosphate (db cyclic AMP) and prostaglandins E1 and E2. 4 The incidence was increased by: (a) acetylcholine, carbachol and physostigmine; and (b) guanosine triphosphate, dibutyryl cyclic guanosine monophosphate (db cyclic GMP), theophylline and 3-isobutyl-1-methyl-xanthine. 5 These findings suggest that head twitching induced by these three treatments arises from a common biochemical mechanism, which may ultimately be a change in favour of cyclic GMP of the balance between this nucleotide and cyclic AMP within appropriate neurones. This imbalance appears to be elicited or increased by 5-hydroxytryptamine and acetylcholine and to be decreased by dopamine, noradrenaline and E prostaglandins. 6 Neither actinomycin D nor cycloheximide, given during the induction of ethanol dependence, altered the incidence of head twitches after ethanol withdrawal.

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Selected References

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  1. Ahlenius S., Carlsson A., Engel J., Svensson T., Södersten P. Antagonism by alpha methyltyrosine of the ethanol-induced stimulation and euphoria in man. Clin Pharmacol Ther. 1973 Jul-Aug;14(4):586–591. doi: 10.1002/cpt1973144part1586. [DOI] [PubMed] [Google Scholar]
  2. BUTCHER R. W., SUTHERLAND E. W. Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine. J Biol Chem. 1962 Apr;237:1244–1250. [PubMed] [Google Scholar]
  3. Beavo J. A., Rogers N. L., Crofford O. B., Hardman J. G., Sutherland E. W., Newman E. V. Effects of xanthine derivatives on lipolysis and on adenosine 3',5'-monophosphate phosphodiesterase activity. Mol Pharmacol. 1970 Nov;6(6):597–603. [PubMed] [Google Scholar]
  4. Blum K., Wallace J. E. Effects of catecholamine synthesis inhibition on ethanol-induced withdrawal symptoms in mice. Br J Pharmacol. 1974 May;51(1):109–111. doi: 10.1111/j.1476-5381.1974.tb09640.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CORNE S. J., PICKERING R. W., WARNER B. T. A method for assessing the effects of drugs on the central actions of 5-hydroxytryptamine. Br J Pharmacol Chemother. 1963 Feb;20:106–120. doi: 10.1111/j.1476-5381.1963.tb01302.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carlsson A., Engel J., Svensson T. H. Inhibition of ethanol-induced excitation in mice and rats by -methyl-p-tyrosine. Psychopharmacologia. 1972;26(3):307–312. doi: 10.1007/BF00422706. [DOI] [PubMed] [Google Scholar]
  7. Collier H. O., Hammond M. D., Schneider C. Biogenic amines and head twitches in mice during ethanol withdrawal. Br J Pharmacol. 1974 Jun;51(2):310–311. doi: 10.1111/j.1476-5381.1974.tb09663.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Collier H. O., Roy A. C. Morphine-like drugs inhibit the stimulation of E prostaglandins of cyclic AMP formation by rat brain homogenate. Nature. 1974 Mar 1;248(5443):24–27. doi: 10.1038/248024a0. [DOI] [PubMed] [Google Scholar]
  9. Daly J. W. Cyclic adenosine 3',5'monophosphate role in the physiology and pharmacology of the central nervous system. Biochem Pharmacol. 1975 Jan 15;24(2):159–164. doi: 10.1016/0006-2952(75)90272-5. [DOI] [PubMed] [Google Scholar]
  10. Engel J., Strömbom U., Svensson T. H., Waldeck B. Suppression by alpha-methyltyrosine of ethanol-induced locomotor stimulation: partial reversal by L-dopa. Psychopharmacologia. 1974 Jul 11;37(3):275–279. doi: 10.1007/BF00421541. [DOI] [PubMed] [Google Scholar]
  11. Ferrendelli J. A., Steiner A. L., McDougal D. B., Jr, Kipnis D. M. The effect of oxotremorine and atropine on cGMP and cAMP levels in mouse cerebral cortex and cerebellum. Biochem Biophys Res Commun. 1970 Nov 25;41(4):1061–1067. doi: 10.1016/0006-291x(70)90193-2. [DOI] [PubMed] [Google Scholar]
  12. French S. W., Palmer D. S., Narod M. E., Reid P. E., Ramey C. W. Noradrenergic sensitivity of the cerebral cortex after chronic ethanol ingestion and withdrawal. J Pharmacol Exp Ther. 1975 Aug;194(2):319–326. [PubMed] [Google Scholar]
  13. Goldstein D. B. Alcohol withdrawal reactions in mice: effects of drugs that modify neurotransmission. J Pharmacol Exp Ther. 1973 Jul;186(1):1–9. [PubMed] [Google Scholar]
  14. Griffiths P. J., Littleton J. M., Ortiz A. Changes in monoamine concentrations in mouse brain associated with ethanol dependence and withdrawal. Br J Pharmacol. 1974 Apr;50(4):489–498. doi: 10.1111/j.1476-5381.1974.tb08582.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Guidotti A., Costa E. A role for nicotinic receptors in the regulation of the adenylate cyclase of adrenal medulla. J Pharmacol Exp Ther. 1974 Jun;189(3):665–675. [PubMed] [Google Scholar]
  16. Gullis R., Traber J., Hamprecht B. Morphine elevates levels of cyclic GMP in a neuroblastoma X glioma hybrid cell line. Nature. 1975 Jul 3;256(5512):57–59. doi: 10.1038/256057a0. [DOI] [PubMed] [Google Scholar]
  17. HALEY T. J., MCCORMICK W. G. Pharmacological effects produced by intracerebral injection of drugs in the conscious mouse. Br J Pharmacol Chemother. 1957 Mar;12(1):12–15. doi: 10.1111/j.1476-5381.1957.tb01354.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hammond M. D., Schneider C. Behavioural changes induced in mice following termination of ethanol administration. Br J Pharmacol. 1973 Mar;47(3):667P–667P. [PMC free article] [PubMed] [Google Scholar]
  19. Hammond M. D., Schneider C. Proceedings: Cyclic nucleotides and ethanol withdrawal head-twitches in mice. Br J Pharmacol. 1974 Sep;52(1):138P–138P. [PMC free article] [PubMed] [Google Scholar]
  20. Hammond M. D. The use of an internal standard in the determination of ethanol in blood, brain and vapour. Br J Addict Alcohol Other Drugs. 1975 Jun;70(2):162–164. doi: 10.1111/j.1360-0443.1975.tb00021.x. [DOI] [PubMed] [Google Scholar]
  21. Hong E. On the antiserotonin activity of 6-acetamido-3(3-(4-phenyl-1-piperazinyl)-propyl)-284(1h,3h)-quinazolinedione-maleate (MA 1420). Arzneimittelforschung. 1973 Dec;23(12):1726–1728. [PubMed] [Google Scholar]
  22. Hunt W. A., Majchrowicz E. Alterations in the turnover of brain norepinephrine and dopamine in alcohol-dependent rats. J Neurochem. 1974 Sep;23(3):549–552. doi: 10.1111/j.1471-4159.1974.tb06058.x. [DOI] [PubMed] [Google Scholar]
  23. Kebabian J. W., Zatz M., Romero J. A., Axelrod J. Rapid changes in rat pineal beta-adrenergic receptor: alterations in l-(3H)alprenolol binding and adenylate cyclase. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3735–3739. doi: 10.1073/pnas.72.9.3735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Mawson C., Whittington H. Evaluation of the peripheral and central antagonistic activities against 5-hydroxytryptamine of some new agents. Br J Pharmacol. 1970 May;39(1):223P–224P. [PMC free article] [PubMed] [Google Scholar]
  25. Mickey J., Tate R., Lefkowitz R. J. Subsensitivity of adenylate cyclase and decreased beta-adrenergic receptor binding after chronic exposure to (minus)-isoproterenol in vitro. J Biol Chem. 1975 Jul 25;250(14):5727–5729. [PubMed] [Google Scholar]
  26. Mukherjee C., Caron M. G., Lefkowitz R. J. Catecholamine-induced subsensitivity of adenylate cyclase associated with loss of beta-adrenergic receptor binding sites. Proc Natl Acad Sci U S A. 1975 May;72(5):1945–1949. doi: 10.1073/pnas.72.5.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Pohorecky L. A. Effects of ethanol on central and peripheral noradrenergic neurons. J Pharmacol Exp Ther. 1974 May;189(2):380–391. [PubMed] [Google Scholar]
  28. SPECTOR S., SJOERDSMA A., UDENFRIEND S. BLOCKADE OF ENDOGENOUS NOREPINEPHRINE SYNTHESIS BY ALPHA-METHYL-TYROSINE, AN INHIBITOR OF TYROSINE HYDROXYLASE. J Pharmacol Exp Ther. 1965 Jan;147:86–95. [PubMed] [Google Scholar]
  29. Wellmann W., Schwabe U. Effects of prostaglandins E1, E2 and F2a on cyclic AMP levels in brain in vivo. Brain Res. 1973 Sep 14;59:371–378. doi: 10.1016/0006-8993(73)90275-8. [DOI] [PubMed] [Google Scholar]

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