Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1976 Sep;58(1):109–116. doi: 10.1111/j.1476-5381.1976.tb07698.x

The effect of catecholamines on the influx of calcium and the development of tension in denervated mouse diaphragm muscle.

R H Evans, J W Smith
PMCID: PMC1667147  PMID: 974368

Abstract

1 The nature of the catecholamine-induced contracture of chronically denervated mouse diaphragm muscle has been investigated and compared with the contractural response evoked by acetylcholine. 2 The time course of onset of catecholamine-sensitivity in denervated diaphragm muscles was similar to the development of acetylcholine sensitivity. However, catecholamine contractures were absent in tissues denervated for periods longer than 90 days whereas acetylcholine-sensitivity was still evident several months after denervation. 3 The catecholamine-induced contracture of the denervated muscle was inhibited specifically by beta-receptor blocking drugs and was unaffected by alpha-receptor blocking drugs and cholinoceptor antagonists. 4 Catecholamine-induced contractures of denervated muscles, unlike contractures to acetylcholine, were dependent upon the presence of spontaneous fibrillation and the amplitude of spontaneous fibrillation was increased by catecholamines. Fibrillation was absent in the presence of tetrodotoxin (1 muM), 2,4-dinitrophenol (10 muM), potassium cyanide (10 muM), ouabain (100 muM), in lithium chloride Ringer solution and at low temperature. Under these conditions catecholamine-induced contractures, but not those to acetylcholine, were abolished. 5 Labelled calcium was found progressively to enter denervated muscle fibres and this entry of calcium was increased by catecholamines. It is suggested that this calcium entry may represent either an increased calcium permeability of denervated muscle fibres which is increased further by catecholamines or the presence of a calcium current that occurs during the fibrillatory potentials of denervated muscle.

Full text

PDF
109

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AXELSSON J., THESLEFF S. A study of supersensitivity in denervated mammalian skeletal muscle. J Physiol. 1959 Jun 23;147(1):178–193. doi: 10.1113/jphysiol.1959.sp006233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERNSTEIN L., INOYE T., PASCAL L. R., RYAN R. J. Experiences with radiosulfate in the estimation of physiologic extracellular water in healthy and abnormal man. J Clin Invest. 1956 Oct;35(10):1119–1130. doi: 10.1172/JCI103366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BIANCHI C. P., SHANES A. M. Calcium influx in skeletal muscle at rest, during activity, and during potassium contracture. J Gen Physiol. 1959 Mar 20;42(4):803–815. doi: 10.1085/jgp.42.4.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BOWMAN W. C., GOLDBERG A. A., RAPER C. A comparison between the effects of a tetanus and the effects of sympathomimetic amines on fast- and slow-contracting mammalian muscles. Br J Pharmacol Chemother. 1962 Dec;19:464–484. doi: 10.1111/j.1476-5381.1962.tb01451.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BOWMAN W. C., RAPER C. Adrenaline and slow-contracting skeletal muscles. Nature. 1962 Jan 6;193:41–43. doi: 10.1038/193041a0. [DOI] [PubMed] [Google Scholar]
  6. BOWMAN W. C., RAPER C. THE EFFECTS OF SYMPATHOMIMETIC AMINES ON CHRONICALLY DENERVATED SKELETAL MUSCLES. Br J Pharmacol Chemother. 1965 Feb;24:98–109. doi: 10.1111/j.1476-5381.1965.tb02083.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. BOWMAN W. C., ZAIMIS E. The effects of adrenaline, noradrenaline and isoprenaline on skeletal muscle contractions in the cat. J Physiol. 1958 Nov 10;144(1):92–107. doi: 10.1113/jphysiol.1958.sp006088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bhoola K. D., Evans R. H., Smith J. W. Catecholamine induced contractures in denervated muscle. Br J Pharmacol. 1972 Nov;46(3):531P–532P. [PMC free article] [PubMed] [Google Scholar]
  9. Bowman W. C., Nott M. W. Actions of sympathomimetic amines and their antagonists on skeletal muscle. Pharmacol Rev. 1969 Mar;21(1):27–72. [PubMed] [Google Scholar]
  10. Brown G. L. The actions of acetylcholine on denervated mammalian and frog's muscle. J Physiol. 1937 Jun 3;89(4):438–461. doi: 10.1113/jphysiol.1937.sp003491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Evans R. H. The entry of labelled calcium into the innervated region of the mouse diaphragm muscle. J Physiol. 1974 Aug;240(3):517–533. doi: 10.1113/jphysiol.1974.sp010621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Freeman S. E., Turner R. J. Ionic interactions in acetylcholine contraction of the denervated rat diaphragm. Br J Pharmacol. 1969 Jul;36(3):510–522. doi: 10.1111/j.1476-5381.1969.tb08007.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kao C. Y. Tetrodotoxin, saxitoxin and their significance in the study of excitation phenomena. Pharmacol Rev. 1966 Jun;18(2):997–1049. [PubMed] [Google Scholar]
  14. Katz B., Miledi R. A study of synaptic transmission in the absence of nerve impulses. J Physiol. 1967 Sep;192(2):407–436. doi: 10.1113/jphysiol.1967.sp008307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LETLEY E. The effect of temperature on the direct muscle twitch response and the action of drugs on the isolated denervated rat diaphragm. Br J Pharmacol Chemother. 1960 Jun;15:345–350. doi: 10.1111/j.1476-5381.1960.tb01254.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MONTAGU K. A. On the mechanism of action of adrenaline in skeletal nerve-muscle. J Physiol. 1955 Jun 28;128(3):619–628. doi: 10.1113/jphysiol.1955.sp005329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mayer S. E., Stull J. T. Cyclic AMP in skeletal muscle. Ann N Y Acad Sci. 1971 Dec 30;185:433–448. doi: 10.1111/j.1749-6632.1971.tb45271.x. [DOI] [PubMed] [Google Scholar]
  18. NARAHASHI T., DEGUCHI T., URAKAWA N., OHKUBO Y. Stabilization and rectification of muscle fiber membrane by tetrodotoxin. Am J Physiol. 1960 May;198:934–938. doi: 10.1152/ajplegacy.1960.198.5.934. [DOI] [PubMed] [Google Scholar]
  19. Purves D., Sakmann B. The effect of contractile activity on fibrillation and extrajunctional acetylcholine-sensitivity in rat muscle maintained in organ culture. J Physiol. 1974 Feb;237(1):157–182. doi: 10.1113/jphysiol.1974.sp010475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sandow A. Excitation-contraction coupling in skeletal muscle. Pharmacol Rev. 1965 Sep;17(3):265–320. [PubMed] [Google Scholar]
  21. Smith J. W., Thesleff S. Spontaneous activity in denervated mouse diaphragm muscle. J Physiol. 1976 May;257(1):171–186. doi: 10.1113/jphysiol.1976.sp011362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. THESLEFF S. Effects of motor innervation on the chemical sensitivity of skeletal muscle. Physiol Rev. 1960 Oct;40:734–752. doi: 10.1152/physrev.1960.40.4.734. [DOI] [PubMed] [Google Scholar]
  23. Turkanis S. A. Some properties of the denervated anterior gracilis muscle of the rat. Br J Pharmacol. 1969 Oct;37(2):414–424. doi: 10.1111/j.1476-5381.1969.tb10578.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WALSER M., SELDIN D. W., GROLLMAN A. Radiosulfate space of muscle. Am J Physiol. 1954 Feb;176(2):322–324. doi: 10.1152/ajplegacy.1954.176.2.322. [DOI] [PubMed] [Google Scholar]
  25. Yamada K., Harigaya S. Contractile response to sympathomimetic amines in isolated rat muscles after chronic denervation. Jpn J Pharmacol. 1974 Jun;24(3):487–490. doi: 10.1254/jjp.24.487. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES