Abstract
1 A lipopolysaccharide extract of Escherichia coli 026:B6 cells (026:B6(B) endotoxin) was shown to be toxic to normal adult guinea-pigs. 2 The agent had no action on isolated preparations of ileum and heart taken from normal adult guinea-pigs. 3 Ileal segments from animals actively immunized against 026:B6(B) endotoxin showed dose-dependent contractions when exposed to endotoxin. Desensitization phenomena were demonstrated. 4 Reactivity of 026:B6(B) endotoxin was transferred to isolated preparations of ileum and heart from normal animals by passive transfer of immune serum. 5 Tissue responses to 026:B6(B) were associated with release of ileal spasmogen into the bath medium. Mepyramine blocked the effects of this spasmogen at bath concentrations which caused little change in ileal responses to carbachol. 6 It is concluded that E. coli endotoxin can elicit anaphylactic reactions, and that this process may potentiate endotoxin toxicity in sensitized animals. However, endotoxin toxicity in guinea-pigs does not appear to depend on this kind of allergic process.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BENNETT I. L., Jr, CLUFF L. E. Bacterial pyrogens. Pharmacol Rev. 1957 Dec;9(4):427–479. [PubMed] [Google Scholar]
- FEIGEN G. A., VAUGHAN WILLIAMS E. M., PETERSON J. K., NIELSEN C. B. Histamine release and intracellular potentials during anaphylaxis in the isolated heart. Circ Res. 1960 Jul;8:713–723. doi: 10.1161/01.res.8.4.713. [DOI] [PubMed] [Google Scholar]
- GILBERT R. P. Mechanisms of the hemodynamic effects of endotoxin. Physiol Rev. 1960 Apr;40:245–279. doi: 10.1152/physrev.1960.40.2.245. [DOI] [PubMed] [Google Scholar]
- King W. W., Cox C. E. Bacterial inhibition of ureteral smooth muscle contractility. I. The effect of common urinary pathogens and endotoxin in an in vitro system. J Urol. 1972 Nov;108(5):700–705. doi: 10.1016/s0022-5347(17)60844-9. [DOI] [PubMed] [Google Scholar]
- MONGAR J. L., SCHILD H. O. Cellular mechanisms in anaphylaxis. Physiol Rev. 1962 Apr;42:226–270. doi: 10.1152/physrev.1962.42.2.226. [DOI] [PubMed] [Google Scholar]
- RASKOVA H., VANECEK J. PHARMACOLOGY OF BACTERIAL TOXINS. Pharmacol Rev. 1964 Mar;16:1–45. [PubMed] [Google Scholar]
- Smith H. W. The development of the flora of the alimentary tract in young animals. J Pathol Bacteriol. 1965 Oct;90(2):495–513. [PubMed] [Google Scholar]
- URBASCHEK B. MOTILITY-PROMOTING EFFECT OF THE BRUCELLA ABORTUS AND BRUCELLA MELITENSIS ENDOTOXIN ON THE SMOOTH UTERINE MUSCLE. Nature. 1964 May 30;202:883–884. doi: 10.1038/202883a0. [DOI] [PubMed] [Google Scholar]
- Urbaschek B., Versteyl R. Increase of the effect of histamine by E. coli endotoxin on the smooth muscle. Nature. 1965 Aug 14;207(998):763–764. doi: 10.1038/207763a0. [DOI] [PubMed] [Google Scholar]