Abstract
1 The possibility of concanavalin A (Con A) blocking the development of desensitization of nicotinic receptors of the cat adrenal gland has been investigated. 2 During perfusion of the adrenal gland with Krebs-bicarbonate solution containing acetylcholine (ACh), the rate of catecholamine (CA) secretion was very high in the first 2 min; thereafter, as perfusion with ACh was continued the output fell, to reach about 20% of the initial value in 10 minutes. When the adrenal gland was pretreated with Con A, the subsequent desensitization of release during continued infusion of ACh was prevented. 3 When the adrenal gland was perfused with high K+ solution, there was always a large initial secretion of CA, and as perfusion with high K+ continued the output fell, to reach about 15% of the initial rate in 10 minutes. Con A did not affect the rate of CA secretion induced by high K+. 4 It is tentatively suggested that Con A blocks the desensitization of CA secretion evoked by ACh by interaction with the glycoprotein moiety of the nicotinic receptor of adrenal chromaffin cells.
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- ANTON A. H., SAYRE D. F. A study of the factors affecting the aluminum oxide-trihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther. 1962 Dec;138:360–375. [PubMed] [Google Scholar]
- DOUGLAS W. W., RUBIN R. P. The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J Physiol. 1961 Nov;159:40–57. doi: 10.1113/jphysiol.1961.sp006791. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dixon W. R., Garcia A. G., Kirpekar S. M. Release of catecholamines and dopamine beta-hydroxylase from the perfused adrenal gland of the cat. J Physiol. 1975 Jan;244(3):805–824. doi: 10.1113/jphysiol.1975.sp010827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Douglas W. W., Poisner A. M. Preferential release of adrenaline from the adrenal medulla by muscarine and pilocarpine. Nature. 1965 Dec 11;208(5015):1102–1103. doi: 10.1038/2081102a0. [DOI] [PubMed] [Google Scholar]
- Hubbard J. I. Microphysiology of vertebrate neuromuscular transmission. Physiol Rev. 1973 Jul;53(3):674–723. doi: 10.1152/physrev.1973.53.3.674. [DOI] [PubMed] [Google Scholar]
- KATZ B., THESLEFF S. A study of the desensitization produced by acetylcholine at the motor end-plate. J Physiol. 1957 Aug 29;138(1):63–80. doi: 10.1113/jphysiol.1957.sp005838. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mathers D. A., Usherwood P. N. Concanavalin A blocks desensitisation of glutamate receptors on insect muscle fibres. Nature. 1976 Feb 5;259(5542):409–411. doi: 10.1038/259409a0. [DOI] [PubMed] [Google Scholar]
- Nicolson G. L. The interactions of lectins with animal cell surfaces. Int Rev Cytol. 1974;39:89–190. doi: 10.1016/s0074-7696(08)60939-0. [DOI] [PubMed] [Google Scholar]
- Rang H. P., Ritter J. M. On the mechanism of desensitization at cholinergic receptors. Mol Pharmacol. 1970 Jul;6(4):357–382. [PubMed] [Google Scholar]
- Rubin R. P., Miele E. A study of the differential secretion of epinephrine and norepinephrine from the perfused cat adrenal gland. J Pharmacol Exp Ther. 1968 Nov;164(1):115–121. [PubMed] [Google Scholar]
- VOGT M. The secretion of the denervated adrenal medulla of the cat. Br J Pharmacol Chemother. 1952 Jun;7(2):325–330. doi: 10.1111/j.1476-5381.1952.tb01329.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson S. P., Kirshner N. The acetylcholine receptor of the adrenal medulla. J Neurochem. 1977 Apr;28(4):687–695. doi: 10.1111/j.1471-4159.1977.tb10615.x. [DOI] [PubMed] [Google Scholar]