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. 1978 Sep;64(1):13–20. doi: 10.1111/j.1476-5381.1978.tb08635.x

Evidence for purinergic innervation of the anococcygeus muscle

G Burnstock, T Cocks, Rahima Crowe
PMCID: PMC1668262  PMID: 698477

Abstract

1 Fluorescence histochemical localization of quinacrine (which binds to adenosine 5′-triphosphate (ATP)) revealed nerve fibres running singly and in bundles in both rat and rabbit anococcygeus muscle. Single neurone cell bodies and ganglia containing between 2 and 50 cells were also observed.

2 Catecholamine fluorescence studies revealed a dense adrenergic ground plexus, but no adrenergic ganglion cells were detected. No acetylcholinesterase-positive nerve fibres or ganglion cells were seen in the rat.

3 When the tone was raised with guanethidine, a relaxation in response to field stimulation was revealed, which was unaffected by atropine but blocked by tetrodotoxin.

4 Release of ATP increased 3 to 6 times above background during stimulation of these non-adrenergic, non-cholinergic, inhibitory nerves.

5 Neither quinacrine staining nor the release of ATP during inhibitory nerve stimulation was affected by 6-hydroxydopamine treatment, which abolished catecholamine fluorescence.

6 Exogenous ATP produced relaxation in high tone preparations of the rabbit anococcygeus muscle. ATP produced either contraction or a small relaxation followed by a contraction of the rat anococcygeus muscle, but treatment with low concentrations of the prostaglandin synthesis inhibitor indomethacin, converted the contraction to a relaxation.

7 These data are consistent with the view that the anococcygeus muscle is innervated by purinergic inhibitory nerves.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BULBRING E. Measurements of oxygen consumption in smooth muscle. J Physiol. 1953 Oct;122(1):111–134. doi: 10.1113/jphysiol.1953.sp004983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennett M. R., Burnstock G., Holman M. Transmission from intramural inhibitory nerves to the smooth muscle of the guinea-pig taenia coli. J Physiol. 1966 Feb;182(3):541–558. doi: 10.1113/jphysiol.1966.sp007836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burnstock G., Campbell G., Satchell D., Smythe A. Evidence that adenosine triphosphate or a related nucleotide is the transmitter substance released by non-adrenergic inhibitory nerves in the gut. Br J Pharmacol. 1970 Dec;40(4):668–688. doi: 10.1111/j.1476-5381.1970.tb10646.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burnstock G., Cocks T., Crowe R., Kasakov L. Purinergic innervation of the guinea-pig urinary bladder. Br J Pharmacol. 1978 May;63(1):125–138. doi: 10.1111/j.1476-5381.1978.tb07782.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burnstock G., Cocks T., Paddle B., Staszewska-Barczak J. Evidence that prostaglandin is responsible for the 'rebound contraction' following stimulation of non-adrenergic, non-cholinergic ('purinergic') inhibitory nerves. Eur J Pharmacol. 1975 Apr;31(2):360–362. doi: 10.1016/0014-2999(75)90060-6. [DOI] [PubMed] [Google Scholar]
  6. Burnstock G. Evolution of the autonomic innervation of visceral and cardiovascular systems in vertebrates. Pharmacol Rev. 1969 Dec;21(4):247–324. [PubMed] [Google Scholar]
  7. Burnstock G. Neural nomenclature. Nature. 1971 Jan 22;229(5282):282–283. doi: 10.1038/229282d0. [DOI] [PubMed] [Google Scholar]
  8. Burnstock G. Purinergic nerves. Pharmacol Rev. 1972 Sep;24(3):509–581. [PubMed] [Google Scholar]
  9. Creed K. E., Gillespie J. S., McCaffery H. The rabbit anococcygeus muscle and its response to field stimulation and to some drugs. J Physiol. 1977 Dec;273(1):121–135. doi: 10.1113/jphysiol.1977.sp012085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Creed K. E., Gillespie J. S. Some electrical properties of the rabbit anococcygeus muscle and a comparison of the effects of inhibitory nerve stimulation in the rat and rabbit. J Physiol. 1977 Dec;273(1):137–153. doi: 10.1113/jphysiol.1977.sp012086. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gillespie J. S., Lüllmann-Rauch R. On the ultrastructure of the rat anococcygeus muscle. Cell Tissue Res. 1974;149(1):91–104. doi: 10.1007/BF00209052. [DOI] [PubMed] [Google Scholar]
  12. Gillespie J. S., McGrath J. C. The response of the cat anococcygeus muscle to nerve or drug stimulation and a comparison with the rat anococcygeus. Br J Pharmacol. 1974 Jan;50(1):109–118. doi: 10.1111/j.1476-5381.1974.tb09597.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gillespie J. S. The rat anococcygeus muscle and its response to nerve stimulation and to some drugs. Br J Pharmacol. 1972 Jul;45(3):404–416. doi: 10.1111/j.1476-5381.1972.tb08097.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HOLTON P. The liberation of adenosine triphosphate on antidromic stimulation of sensory nerves. J Physiol. 1959 Mar 12;145(3):494–504. doi: 10.1113/jphysiol.1959.sp006157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. IRVIN J. L., IRVIN E. M. The interaction of quinacrine with adenine nucleotides. J Biol Chem. 1954 Sep;210(1):45–56. [PubMed] [Google Scholar]
  16. Johansen T., Lewis G. P. Dependence of histamine release from rat mast cells induced by the ionophore A23187 on endogenous adenosine triphosphate [proceedings]. Br J Pharmacol. 1977 Mar;59(3):474P–475P. [PMC free article] [PubMed] [Google Scholar]
  17. KARNOVSKY M. J., ROOTS L. A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES. J Histochem Cytochem. 1964 Mar;12:219–221. doi: 10.1177/12.3.219. [DOI] [PubMed] [Google Scholar]
  18. Langer S. Z., Pinto J. E. Possible involvement of a transmitter different from norepinephrine in the residual responses to nerve stimulation of the cat nictitating membrane after pretreatment with reserpine. J Pharmacol Exp Ther. 1976 Mar;196(3):697–713. [PubMed] [Google Scholar]
  19. MCELROY W. D., SELIGER H. E. THE CHEMISTRY OF LIGHT EMISSION. Adv Enzymol Relat Areas Mol Biol. 1963;25:119–166. doi: 10.1002/9780470122709.ch3. [DOI] [PubMed] [Google Scholar]
  20. Needleman P., Minkes M. S., Douglas J. R., Jr Stimulation of prostaglandin biosynthesis by adenine nucleotides. Profile of prostaglandin release by perfused organs. Circ Res. 1974 Apr;34(4):455–460. doi: 10.1161/01.res.34.4.455. [DOI] [PubMed] [Google Scholar]
  21. Olson L., Alund M., Norberg K. A. Fluorescence-microscopical demonstration of a population of gastro-intestinal nerve fibres with a selective affinity for quinacrine. Cell Tissue Res. 1976 Sep 1;171(4):407–423. doi: 10.1007/BF00220234. [DOI] [PubMed] [Google Scholar]
  22. Smith A. D. Subcellular localisation of noradrenaline in sympathetic neurons. Pharmacol Rev. 1972 Sep;24(3):435–457. [PubMed] [Google Scholar]
  23. Strehler B. L. Bioluminescence assay: principles and practice. Methods Biochem Anal. 1968;16:99–181. doi: 10.1002/9780470110348.ch2. [DOI] [PubMed] [Google Scholar]
  24. Vane J. R. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol. 1971 Jun 23;231(25):232–235. doi: 10.1038/newbio231232a0. [DOI] [PubMed] [Google Scholar]
  25. Wai S. S., Coupar I. M. The inhibitory response of the rabbit anococcygeus muscle. Clin Exp Pharmacol Physiol. 1976 Mar-Apr;3(2):141–145. doi: 10.1111/j.1440-1681.1976.tb00598.x. [DOI] [PubMed] [Google Scholar]

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