Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1978 May;63(1):167–176. doi: 10.1111/j.1476-5381.1978.tb07786.x

Effects of prostaglandin E2 and a prostaglandin endoperoxide analogue on neuroeffector transmission in the rat anococcygeus muscle

Khawla S Al Timimi, JR Bedwani, AWB Stanton
PMCID: PMC1668280  PMID: 206303

Abstract

1 Investigations were made into the effects of prostaglandin E2 (PGE2) and a prostaglandin endoperoxide analogue (Upjohn compound U-46619) on the responses of the rat anococcygeus muscle to field stimulation of the intrinsic sympathetic nerves, and to exogenous noradrenaline. The effects of PGE2 on responses to stimulation of intrinsic inhibitory nerves were also studied.

2 PGE2 (5.6 × 10-8 or 2.8 × 10-6 mol/l) decreased motor (sympathetic) responses to field stimulation at all frequencies tested (2 to 24 Hz). The prostaglandin also reduced the inhibitory responses to field stimulation, seen when the tone of the preparation had been raised and its sympathetic innervation had been blocked by guanethidine. However, these inhibitory responses were also reduced by other spasmogens (carbachol and 5-hydroxytryptamine) which, like PGE2, further increased the tone of guanethidine-treated preparations.

3 At a concentration of 5.6 × 10-8 mol/l, PGE2 had no effect on responses to noradrenaline, whereas at a fifty-fold higher concentration the prostaglandin potentiated these.

4 Unlike PGE2, U-46619 (5.6 × 10-8 mol/l) greatly potentiated motor responses to field stimulation, at frequencies from 0.75 to 24 Hz. This effect did not represent a specific facilitation of sympathetic neurotransmission, as responses to carbachol and 5-hydroxytryptamine, as well as to noradrenaline, were also potentiated.

5 The results are discussed in relation to the effects of prostaglandins and prostaglandin endoperoxides on neuroeffector transmission in other sympathetically innervated tissues. It is concluded that PGE2 inhibits sympathetic neurotransmission in the rat anococcygenus muscle by a prejunctional action, whereas the predominant effect of U-46619 is direct excitation of the muscle. The effect of PGE2 on inhibitory responses to field stimulation may represent an interference with inhibitory neuroeffector transmission in this tissue, or may simply be a consequence of the spasmogenic action of the prostaglandin.

Full text

PDF
167

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambache N., Zar M. A. An inhibitory effect of prostaglandin E2 on neuromuscular transmission in the guinea-pig vas deferens. J Physiol. 1970 May;208(1):30P–32P. [PubMed] [Google Scholar]
  2. Baum T., Shropshire A. T. Influence of prostaglandins on autonomic responses. Am J Physiol. 1971 Nov;221(5):1470–1475. doi: 10.1152/ajplegacy.1971.221.5.1470. [DOI] [PubMed] [Google Scholar]
  3. Bedwani J. R., Millar G. C. Prostaglandin release from cat and dog spleen. Br J Pharmacol. 1975 Aug;54(4):499–505. doi: 10.1111/j.1476-5381.1975.tb07597.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bergström S., Farnebo L. O., Fuxe K. Effect of prostaglandin E 2 on central and peripheral catecholamine neurons. Eur J Pharmacol. 1973 Mar;21(3):362–368. doi: 10.1016/0014-2999(73)90139-8. [DOI] [PubMed] [Google Scholar]
  5. Brody M. J., Kadowitz P. J. Prostaglandins as modulators of the autonomic nervous system. Fed Proc. 1974 Jan;33(1):48–60. [PubMed] [Google Scholar]
  6. Brundin J. The effect of prostaglandin E1 on the response of the rabbit oviduct to hypogastric nerve stimulation. Acta Physiol Scand. 1968 May-Jun;73(1):54–57. doi: 10.1111/j.1748-1716.1968.tb04081.x. [DOI] [PubMed] [Google Scholar]
  7. Chijimatsu Y., Nguyen T. V., Said S. I. Effects of prostaglandin endoperoxide analogs on contractile elements in lung and gastrointestinal tract. Prostaglandins. 1977 May;13(5):909–916. doi: 10.1016/0090-6980(77)90220-9. [DOI] [PubMed] [Google Scholar]
  8. Creed K. E., Gillespie J. S., Muir T. C. The electrical basis of excitation and inhibition in the rat anoccygeus muscle. J Physiol. 1975 Feb;245(1):33–47. doi: 10.1113/jphysiol.1975.sp010833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Davies B. N., Horton E. W., Withrington P. G. The occurrence of prostaglandin E2 in splenic venous blood of the dog following splenic nerve stimulation. Br J Pharmacol Chemother. 1968 Jan;32(1):127–135. doi: 10.1111/j.1476-5381.1968.tb00436.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ferreira S. H., Moncada S., Vane J. R. Some effects of inhibiting endogenous prostaglandin formation on the responses of the cat spleen. Br J Pharmacol. 1973 Jan;47(1):48–58. doi: 10.1111/j.1476-5381.1973.tb08157.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frame M. H., Hedqvist P., Aström A. Effect of prostaglandin E2 on vascular responses of the rabbit kidney to nerve stimulation and noradrenaline, in vitro and in situ. Life Sci. 1974 Jul 15;15(2):239–244. doi: 10.1016/0024-3205(74)90212-4. [DOI] [PubMed] [Google Scholar]
  12. Gillespie J. S., McKnight A. T. The action of some vasoactive polypeptides and their antagonists on the anococcygeus muscle [proceedings]. J Physiol. 1976 Sep;260(2):19P–20P. [PubMed] [Google Scholar]
  13. Gillespie J. S. The rat anococcygeus muscle and its response to nerve stimulation and to some drugs. Br J Pharmacol. 1972 Jul;45(3):404–416. doi: 10.1111/j.1476-5381.1972.tb08097.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gilmore N., Vane J. R., Wyllie J. H. Prostaglandins released by the spleen. Nature. 1968 Jun 22;218(5147):1135–1140. doi: 10.1038/2181135a0. [DOI] [PubMed] [Google Scholar]
  15. Hamberg M., Svensson J., Wakabayashi T., Samuelsson B. Isolation and structure of two prostaglandin endoperoxides that cause platelet aggregation. Proc Natl Acad Sci U S A. 1974 Feb;71(2):345–349. doi: 10.1073/pnas.71.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hedqvist P. Basic mechanisms of prostaglandin action on autonomic neurotransmission. Annu Rev Pharmacol Toxicol. 1977;17:259–279. doi: 10.1146/annurev.pa.17.040177.001355. [DOI] [PubMed] [Google Scholar]
  17. Hedqvist P., Brundin J. Inhibition by prostaglandin E1 of noradrenaline release and of effector response to nerve stimulation in the cat spleen. Life Sci. 1969 Apr 1;8(7):389–395. doi: 10.1016/0024-3205(69)90232-x. [DOI] [PubMed] [Google Scholar]
  18. Hedqvist P. Control by prostaglandin E2 of sympathetic neurotrans-mission in the spleen. Life Sci. 1970 Mar 1;9(5):269–278. doi: 10.1016/0024-3205(70)90028-7. [DOI] [PubMed] [Google Scholar]
  19. Hedqvist P. Prostaglandin action on transmitter release of adrenergic neuroeffector junctions. Adv Prostaglandin Thromboxane Res. 1976;1:357–363. [PubMed] [Google Scholar]
  20. Hedqvist P. Prostaglandin induced inhibition of neurotransmission in the isolated guinea pig seminal vesicle. Acta Physiol Scand. 1972 Apr;84(4):506–511. doi: 10.1111/j.1748-1716.1972.tb05201.x. [DOI] [PubMed] [Google Scholar]
  21. Hedqvist P., Stjärne L., Wennmalm A. Facilitation of sympathetic neurotransmission in the cat spleen after inhibition of prostaglandin synthesis. Acta Physiol Scand. 1971 Nov;83(3):430–432. doi: 10.1111/j.1748-1716.1971.tb05099.x. [DOI] [PubMed] [Google Scholar]
  22. Hedqvist P., Stjärne L., Wennmalm A. Inhibition by prostaglandin E2 of sympathetic neurotransmission in the rabbit heart. Acta Physiol Scand. 1970 May;79(1):139–141. doi: 10.1111/j.1748-1716.1970.tb04712.x. [DOI] [PubMed] [Google Scholar]
  23. Hedqvist P. Studies on the effect of prostaglandins E1 and E2 on the sympathetic neuromuscular transmission in some animal tissues. Acta Physiol Scand Suppl. 1970;345:1–40. [PubMed] [Google Scholar]
  24. Hedqvist P., Von Euler U. S. Prostaglandin-induced neurotransmission failure in the field-stimulated, isolated vas deferens. Neuropharmacology. 1972 Mar;11(2):177–187. doi: 10.1016/0028-3908(72)90090-1. [DOI] [PubMed] [Google Scholar]
  25. Hedqvist P., von Euler U. S. Prostaglandin controls neuromuscular transmission in guinea-pig vas deferens. Nat New Biol. 1972 Mar 29;236(65):113–115. doi: 10.1038/newbio236113a0. [DOI] [PubMed] [Google Scholar]
  26. Moawad A., Hedqvist P., Bygdeman M. Noradrenaline release following nerve stimulation and its modification by prostaglandin E2 in human and rabbit oviduct. Acta Physiol Scand. 1975 Sep;95(1):142–144. doi: 10.1111/j.1748-1716.1975.tb10036.x. [DOI] [PubMed] [Google Scholar]
  27. Samuelsson B., Wennmalm A. Increased nerve stimulation induced release of noradrenaline from the rabbit heart after inhibition of prostaglandin synthesis. Acta Physiol Scand. 1971 Oct;83(2):163–168. doi: 10.1111/j.1748-1716.1971.tb05065.x. [DOI] [PubMed] [Google Scholar]
  28. Stjärne L. Prostaglandin-versus alpha-adrenoceptor-mediated control of sympathetic neurotransmitter secretion in guinea-pig isolated vas deferens. Eur J Pharmacol. 1973 Jun;22(3):233–238. doi: 10.1016/0014-2999(73)90021-6. [DOI] [PubMed] [Google Scholar]
  29. Wasserman M. A. Bronchopulmonary pharmacology of some prostaglandin endoperoxide analogs in the dog. Eur J Pharmacol. 1976 Mar;36(1):103–114. doi: 10.1016/0014-2999(76)90262-4. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES