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. 1979 Jan;65(1):103–109. doi: 10.1111/j.1476-5381.1979.tb17338.x

Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187.

T Johansen
PMCID: PMC1668482  PMID: 83892

Abstract

The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylnydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRIERLEY G. P., MURER E., BACHMANN E. STUDIES ON ION TRANSPORT. III. THE ACCUMULATION OF CALCIUM AND INORGANIC PHOSPHATE BY HEART MITOCHONDRIA. Arch Biochem Biophys. 1964 Apr;105:89–102. doi: 10.1016/0003-9861(64)90239-5. [DOI] [PubMed] [Google Scholar]
  2. BRIERLEY G. P., MURER E., GREEN D. E. Participation of an intermediate of oxidative phosphorylation in ion accumulation by mitochondria. Science. 1963 Apr 5;140(3562):60–62. doi: 10.1126/science.140.3562.60. [DOI] [PubMed] [Google Scholar]
  3. Bielawski J., Lehninger A. L. Stoichiometric relationships in mitochondrial accumulation of calcium and phosphate supported by hydrolysis of adenosine triphosphate. J Biol Chem. 1966 Oct 10;241(19):4316–4322. [PubMed] [Google Scholar]
  4. Carafoli E., Lehninger A. L. A survey of the interaction of calcium ions with mitochondria from different tissues and species. Biochem J. 1971 May;122(5):681–690. doi: 10.1042/bj1220681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Diamant B., Lowry O. H. Dry weight determination of single lyophilized mast cells of the rat. J Histochem Cytochem. 1966 Jul;14(7):519–524. doi: 10.1177/14.7.519. [DOI] [PubMed] [Google Scholar]
  6. Diamant B., Norn S., Felding P., Olsen N., Ziebell A., Nissen J. ATP level and CO2 production of mast cells in anaphylaxis. Int Arch Allergy Appl Immunol. 1974;47(6):894–908. doi: 10.1159/000231280. [DOI] [PubMed] [Google Scholar]
  7. Foreman J. C., Mongar J. L., Gomperts B. D. Calcium ionophores and movement of calcium ions following the physiological stimulus to a secretory process. Nature. 1973 Oct 5;245(5423):249–251. doi: 10.1038/245249a0. [DOI] [PubMed] [Google Scholar]
  8. HUIJING F., SLATER E. C. The use of oligomycin as an inhibitor of oxidative phosphorylation. J Biochem. 1961 Jun;49:493–501. doi: 10.1093/oxfordjournals.jbchem.a127334. [DOI] [PubMed] [Google Scholar]
  9. Johansen T., Chakravarty N. Dependence of histamine release from rat mast cells on adenosine triphosphate. Naunyn Schmiedebergs Arch Pharmacol. 1972;275(4):457–463. doi: 10.1007/BF00501133. [DOI] [PubMed] [Google Scholar]
  10. Johansen T., Chakravarty N. The utilization of adenosine triphosphate in rat mast cells during histamine release induced by anaphylactic reaction and compound 48/80. Naunyn Schmiedebergs Arch Pharmacol. 1975;288(2-3):243–260. doi: 10.1007/BF00500530. [DOI] [PubMed] [Google Scholar]
  11. Johansen T. Mechanism of histamine release from rat mast cells induced by the ionophore A23187: effects of calcium and temperature. Br J Pharmacol. 1978 Aug;63(4):643–649. doi: 10.1111/j.1476-5381.1978.tb17277.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LARDY H. A., CONNELLY J. L., JOHNSON D. ANTIBIOTIC STUDIES. II. INHIBITION OF PHOSPHORYL TRANSFER IN MITOCHONDRIA BY OLIGOMYCIN AND AUROVERTIN. Biochemistry. 1964 Dec;3:1961–1968. doi: 10.1021/bi00900a030. [DOI] [PubMed] [Google Scholar]
  13. Lehninger A. L., Carafoli E., Rossi C. S. Energy-linked ion movements in mitochondrial systems. Adv Enzymol Relat Areas Mol Biol. 1967;29:259–320. doi: 10.1002/9780470122747.ch6. [DOI] [PubMed] [Google Scholar]
  14. Lehninger A. L. Mitochondria and calcium ion transport. Biochem J. 1970 Sep;119(2):129–138. doi: 10.1042/bj1190129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Malmström K., Carafoli E. The interaction of Ga2+ with mitochondria from human myometrium. Arch Biochem Biophys. 1977 Aug;182(2):657–666. doi: 10.1016/0003-9861(77)90546-x. [DOI] [PubMed] [Google Scholar]
  16. ROSSI C. S., LEHNINGER A. L. STOICHIOMETRIC RELATIONSHIPS BETWEEN ACCUMULATION OF IONS BY MITOCHONDRIA AND THE ENERGY-COUPLING SITES IN THE RESPIRATORY CHAIN. Biochem Z. 1963;338:698–713. [PubMed] [Google Scholar]
  17. ROSSI C. S., LEHNINGER A. L. STOICHIOMETRY OF RESPIRATORY STIMULATION, ACCUMULATION OF CA++ AND PHOSPHATE, AND OXIDATIVE PHOSPHORYLATION IN RAT LIVER MITOCHONDRIA. J Biol Chem. 1964 Nov;239:3971–3980. [PubMed] [Google Scholar]
  18. SHORE P. A., BURKHALTER A., COHN V. H., Jr A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther. 1959 Nov;127:182–186. [PubMed] [Google Scholar]
  19. VASINGTON F. D., MURPHY J. V. Ca ion uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation. J Biol Chem. 1962 Aug;237:2670–2677. [PubMed] [Google Scholar]

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