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. 1979 Feb;65(2):215–222. doi: 10.1111/j.1476-5381.1979.tb07821.x

α- and β-Adrenoceptors in the detrusor muscle and bladder base of the pig and β-adrenoceptors in the detrusor muscle of man

Jens-Jørgen Larsen
PMCID: PMC1668624  PMID: 216452

Abstract

1 The presence and type of adrenoceptors in the smooth muscle of the pig and human urinary bladder was assessed on the basis of the relative potency of α- and β-adrenoceptor agonists and antagonists.

2 In isolated, carbachol-contracted bladder strips from the pig detrusor muscle the relaxing potency of isoprenaline was four times that of salbutamol and ritodrine and thirty times that of noradrenaline.

3 Propranolol caused a parallel shift to the right of the noradrenaline dose-response curve which was not changed by phentolamine.

4 Propranolol and butoxamine showed, in contrast to practolol, a dose-dependent antagonism of the response to isoprenaline. A pA2 value of 9.2 ± 0.2 and 6.8 ± 0.2 (mean ± s.e. mean) for the first two antagonists was calculated.

5 In the bladder base of the pig, propranolol caused a parallel shift to the right and phentolamine a shift to the left of the dose-response curve to noradrenaline.

6 In the human detrusor muscle the potency and maximum effect of isoprenaline and salbutamol were less than those in the pig detrusor muscle. The potency of isoprenaline was sixty times that of salbutamol.

7 Whereas a parallel shift to the right of the dose-response curve to isoprenaline was obtained with propranolol, no antagonism was obtained with butoxamine or practolol.

8 The results are interpreted as indicating the presence of β2-adrenoceptors in the detrusor muscle of the pig and β-adrenoceptors with neither β1- nor β2-characteristics in the detrusor muscle of man. An indication of the presence of α-adrenoceptors in the bladder base but not in the detrusor muscle of the pig was obtained.

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Selected References

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  1. ARUNLAKSHANA O., SCHILD H. O. Some quantitative uses of drug antagonists. Br J Pharmacol Chemother. 1959 Mar;14(1):48–58. doi: 10.1111/j.1476-5381.1959.tb00928.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Almgren O., Jonason J. Relative importance of neuronal and extraneuronal mechanisms for the uptake and retention of noradrenaline in different tissues of the rat. Naunyn Schmiedebergs Arch Pharmakol. 1971;270(3):289–309. doi: 10.1007/BF00997028. [DOI] [PubMed] [Google Scholar]
  3. Ambache N., Zar M. A. Non-cholinergic transmission by post-ganglionic motor neurones in the mammalian bladder. J Physiol. 1970 Oct;210(3):761–783. doi: 10.1113/jphysiol.1970.sp009240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Black J. W., Duncan W. A., Shanks R. G. Comparison of some properties of pronethalol and propranolol. Br J Pharmacol Chemother. 1965 Dec;25(3):577–591. doi: 10.1111/j.1476-5381.1965.tb01782.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boyarsky S., Labay P., Gregg R., Levie B. Pharmacologic studies of the nature of the sympathetic nerves of the urinary bladder. Paraplegia. 1968 Nov;6(3):136–150. doi: 10.1038/sc.1968.25. [DOI] [PubMed] [Google Scholar]
  6. Burnstock G., Dumsday B., Smythe A. Atropine resistant excitation of the urinary bladder: the possibility of transmission via nerves releasing a purine nucleotide. Br J Pharmacol. 1972 Mar;44(3):451–461. doi: 10.1111/j.1476-5381.1972.tb07283.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CARPENTER F. G. Excitation of rat urinary bladder by coaxial electrodes and by chemical agents. Am J Physiol. 1963 Apr;204:727–731. doi: 10.1152/ajplegacy.1963.204.4.727. [DOI] [PubMed] [Google Scholar]
  8. CARPENTER F. G., ROOT W. S. Effect of parasympathetic denervation on feline bladder function. Am J Physiol. 1951 Sep;166(3):686–691. doi: 10.1152/ajplegacy.1951.166.3.686. [DOI] [PubMed] [Google Scholar]
  9. CLARK B. B., URSILLO R. C. The action of atropine on the urinary bladder of the dog and on the isolated nerve-bladder strip preparation of the rabbit. J Pharmacol Exp Ther. 1956 Nov;118(3):338–347. [PubMed] [Google Scholar]
  10. Coutinho E. M., De Sousa M. B., Wilson K. H., Landesman R. Inhibitory action of a new sympathomimetic amino (DU-21220) on the nongravid uterus. Am J Obstet Gynecol. 1969 Aug 1;104(7):1053–1056. doi: 10.1016/0002-9378(69)90702-9. [DOI] [PubMed] [Google Scholar]
  11. Daly M. J., Farmer J. B., Levy G. P. Comparison of the bronchodilator and cardiovascular actions of salbutamol, isoprenaline and orciprenaline in guinea-pigs and dogs. Br J Pharmacol. 1971 Nov;43(3):624–638. doi: 10.1111/j.1476-5381.1971.tb07192.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dumsday B. Atropine-resistance of the urinary bladder innervation. J Pharm Pharmacol. 1971 Mar;23(3):222–225. doi: 10.1111/j.2042-7158.1971.tb08649.x. [DOI] [PubMed] [Google Scholar]
  13. Dunlop D., Shanks R. G. Selective blockade of adrenoceptive beta receptors in the heart. Br J Pharmacol Chemother. 1968 Jan;32(1):201–218. doi: 10.1111/j.1476-5381.1968.tb00444.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Edvardsen P. Nervous control of urinary bladder in cats. A survey of recent experimental results and their relation to clinical problems. Acta Neurol Scand. 1967;43(5):543–563. doi: 10.1111/j.1600-0404.1967.tb05550.x. [DOI] [PubMed] [Google Scholar]
  15. Edvardsen P., Setekleiv J. Distribution of adrenergic receptors in the urinary bladder of cats, rabbits, and guinea-pigs. Acta Pharmacol Toxicol (Copenh) 1968;26(5):437–445. doi: 10.1111/j.1600-0773.1968.tb00462.x. [DOI] [PubMed] [Google Scholar]
  16. Elmér M. Inhibitory beta-adrenoceptors in the urinary bladder of the rat. Life Sci. 1974 Jul 15;15(2):273–280. doi: 10.1016/0024-3205(74)90217-3. [DOI] [PubMed] [Google Scholar]
  17. Farmer J. B., Kennedy I., Levy G. P., Marshall R. J. A comparison of the beta-adrenoreceptor stimulant properties of isoprenaline, with those of orciprenaline, salbutamol, soterenol and trimetoquinol on isolated atria and trachea of the guinea pig. J Pharm Pharmacol. 1970 Jan;22(1):61–63. doi: 10.1111/j.2042-7158.1970.tb08388.x. [DOI] [PubMed] [Google Scholar]
  18. Farmer J. B., Levy G. P., Marshall R. J. A comparison of the beta-adrenoceptor stimulant properties of salbutamol, orciprenaline and soterenol with those of isoprenaline. J Pharm Pharmacol. 1970 Dec;22(12):945–947. doi: 10.1111/j.2042-7158.1970.tb08481.x. [DOI] [PubMed] [Google Scholar]
  19. Foster R. W. A correlation between inhibition of the uptake of 3H from (+)-3H-noradrenaline and potentiation of the responses to (-)-noradrenaline in the guinea-pig isolated trachea. Br J Pharmacol Chemother. 1968 Jun;33(2):357–367. doi: 10.1111/j.1476-5381.1968.tb00996.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Foster R. W. An uptake of radioactivity from (plus or minus)-3H-isoprenaline and its inhibition by drugs which potentiate the responses to (minus)-isoprenaline in the guinea-pig isolated trachea. Br J Pharmacol. 1969 Mar;35(3):418–427. doi: 10.1111/j.1476-5381.1969.tb08283.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Furchgott R. F. The pharmacological differentiation of adrenergic receptors. Ann N Y Acad Sci. 1967 Feb 10;139(3):553–570. doi: 10.1111/j.1749-6632.1967.tb41229.x. [DOI] [PubMed] [Google Scholar]
  22. Hald T., Agrawal G., Kantrowitz A. Studies in stimulation of the bladder and its motor nerves. Surgery. 1966 Oct;60(4):848–856. [PubMed] [Google Scholar]
  23. LANDS A. M., BROWN T. G., Jr A COMPARISON OF THE CARDIAC STIMULATING AND BRONCHODILATOR ACTIONS OF SELECTED SYMPATHOMIMETIC AMINES. Proc Soc Exp Biol Med. 1964 Jun;116:331–333. doi: 10.3181/00379727-116-29239. [DOI] [PubMed] [Google Scholar]
  24. Lands A. M., Arnold A., McAuliff J. P., Luduena F. P., Brown T. G., Jr Differentiation of receptor systems activated by sympathomimetic amines. Nature. 1967 May 6;214(5088):597–598. doi: 10.1038/214597a0. [DOI] [PubMed] [Google Scholar]
  25. Lands A. M., Luduena F. P., Buzzo H. J. Differentiation of receptors responsive to isoproterenol. Life Sci. 1967 Nov 1;6(21):2241–2249. doi: 10.1016/0024-3205(67)90031-8. [DOI] [PubMed] [Google Scholar]
  26. Levy B. The adrenergic blocking activity of N-tert.-butylmethoxamine (butoxamine). J Pharmacol Exp Ther. 1966 Mar;151(3):413–422. [PubMed] [Google Scholar]
  27. Maxwell R. A., Ferris R. M., Burcsu J., Woodward E. C., Tang D., Williard K. The phenyl rings of tricyclic antidepressants and related compounds as determinants of the potency of inhibition of the amine pumps in adrenergic neurons of the rabbit aorta and in rat cortical synaptosomes. J Pharmacol Exp Ther. 1974 Dec;191(3):418–430. [PubMed] [Google Scholar]
  28. Nergårdh A., Boréus L. O. Autonomic receptor function in the lower urinary tract of man and cat. Scand J Urol Nephrol. 1972;6(1):32–36. doi: 10.3109/00365597209132077. [DOI] [PubMed] [Google Scholar]
  29. Nergårdh A., Boréus L. O., Naglo A. S. Characterization of the adrenergic beta-receptor in the urinary bladder of man and cat. Acta Pharmacol Toxicol (Copenh) 1977 Jan;40(1):14–21. doi: 10.1111/j.1600-0773.1977.tb02049.x. [DOI] [PubMed] [Google Scholar]
  30. Taira N. The autonomic pharmacology of the bladder. Annu Rev Pharmacol. 1972;12:197–208. doi: 10.1146/annurev.pa.12.040172.001213. [DOI] [PubMed] [Google Scholar]
  31. VANOV S. RESPONSES OF THE RAT URINARY BLADDER IN SITU TO DRUGS AND TO NERVE STIMULATION. Br J Pharmacol Chemother. 1965 Apr;24:591–600. doi: 10.1111/j.1476-5381.1965.tb01750.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wasserman M. A., Levy B. Selective beta adrenergic receptor blockade in the rat. J Pharmacol Exp Ther. 1972 Aug;182(2):256–263. [PubMed] [Google Scholar]
  33. de Sy W., Lacroix E., Leusen I. An analysis of the urinary bladder response to hypogastric nerve stimulation in the cat. Invest Urol. 1974 May;11(6):508–516. [PubMed] [Google Scholar]

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