Abstract
The phytopathogenic fungus Gibberella fujikuroi mating population A (anamorph, Fusarium moniliforme) produces fumonisins, which are toxic to a wide range of plant and animal species. Previous studies of field strains have identified a genetic locus, designated fum1, that can determine whether fumonisins are produced. To test the relationship between fumonisin production and virulence on maize seedlings, a cross between a fum1+ field strain that had a high degree of virulence and a fum1- field strain that had a low degree of virulence was made, and ascospore progeny were scored for these traits. Although a range of virulence levels was recovered among the progeny, high levels of virulence were associated with production of fumonisins, and highly virulent, fumonisin-nonproducing progeny were not obtained. A survey of field strains did identify a rare fumonisin-nonproducing strain that was quite high in virulence. Also, the addition of purified fumonisin B1 to virulence assays did not replicate all of the seedling blight symptoms obtained with autoclaved culture material containing fumonisin. These results support the hypothesis that fumonisin plays a role in virulence but also indicate that fumonisin production is not necessary or sufficient for virulence on maize seedlings.
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Selected References
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- Desjardins A. E., Hohn T. M., McCormick S. P. Trichothecene biosynthesis in Fusarium species: chemistry, genetics, and significance. Microbiol Rev. 1993 Sep;57(3):595–604. doi: 10.1128/mr.57.3.595-604.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Desjardins A. E., Plattner R. D., Shackelford D. D., Leslie J. F., Nelson P. E. Heritability of fumonisin B1 production in Gibberella fujikuroi mating population A. Appl Environ Microbiol. 1992 Sep;58(9):2799–2805. doi: 10.1128/aem.58.9.2799-2805.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dupuy J., Le Bars P., Boudra H., Le Bars J. Thermostability of Fumonisin B(1), a Mycotoxin from Fusarium moniliforme, in Corn. Appl Environ Microbiol. 1993 Sep;59(9):2864–2867. doi: 10.1128/aem.59.9.2864-2867.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kathariou S., Spieth P. T. Spore Killer Polymorphism in FUSARIUM MONILIFORME. Genetics. 1982 Sep;102(1):19–24. doi: 10.1093/genetics/102.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klittich C., Leslie J. F. Nitrate reduction mutants of fusarium moniliforme (gibberella fujikuroi). Genetics. 1988 Mar;118(3):417–423. doi: 10.1093/genetics/118.3.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leslie J. F., Doe F. J., Plattner R. D., Shackelford D. D., Jonz J. Fumonisin B1 production and vegetative compatibility of strains from Gibberella fujikuroi mating population 'A' (Fusarium moniliforme). Mycopathologia. 1992 Feb;117(1-2):37–45. doi: 10.1007/BF00497277. [DOI] [PubMed] [Google Scholar]
- Merrill A. H., Jr Cell regulation by sphingosine and more complex sphingolipids. J Bioenerg Biomembr. 1991 Feb;23(1):83–104. doi: 10.1007/BF00768840. [DOI] [PubMed] [Google Scholar]
- Nelson P. E., Plattner R. D., Shackelford D. D., Desjardins A. E. Production of fumonisins by Fusarium moniliforme strains from various substrates and geographic areas. Appl Environ Microbiol. 1991 Aug;57(8):2410–2412. doi: 10.1128/aem.57.8.2410-2412.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson P. E. Taxonomy and biology of Fusarium moniliforme. Mycopathologia. 1992 Feb;117(1-2):29–36. doi: 10.1007/BF00497276. [DOI] [PubMed] [Google Scholar]
- Panaccione D. G., Scott-Craig J. S., Pocard J. A., Walton J. D. A cyclic peptide synthetase gene required for pathogenicity of the fungus Cochliobolus carbonum on maize. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6590–6594. doi: 10.1073/pnas.89.14.6590. [DOI] [PMC free article] [PubMed] [Google Scholar]