Abstract
The principal objective of this study was to quantify the bioavailability of micelle-solubilized naphthalene to naphthalene-degrading microorganisms comprising a mixed population isolated from contaminated waste and soils. Two nonionic surfactants were used, an alkylethoxylate, Brij 30 (C12E4), and an alkylphenol ethoxylate, Triton X-100 (C8PE9.5). Batch experiments were used to evaluate the effects of aqueous, micellized nonionic surfactants on the microbial mineralization of naphthalene and salicylic acid, an intermediate compound formed in the pathway of microbial degradation of naphthalene. The extent of solubilization and biodegradation under aerobic conditions was monitored by radiotracer and spectrophotometric techniques. Experimental results showed that surfactant concentrations above the critical micelle concentration were not toxic to the naphthalene-degrading bacteria and that the presence of surfactant micelles did not inhibit mineralization of naphthalene. Naphthalene solubilized by micelles of Brij 30 or Triton X-100 in liquid media was bioavailable and degradable by the mixed culture of bacteria.
Full Text
The Full Text of this article is available as a PDF (214.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Breuil C., Kushner D. J. Effects of lipids, fatty acids, and other detergents on bacterial utilization of hexadecane. Can J Microbiol. 1980 Feb;26(2):223–-31. doi: 10.1139/m80-034. [DOI] [PubMed] [Google Scholar]
- Cerniglia C. E. Microbial metabolism of polycyclic aromatic hydrocarbons. Adv Appl Microbiol. 1984;30:31–71. doi: 10.1016/s0065-2164(08)70052-2. [DOI] [PubMed] [Google Scholar]
- Davies J. I., Evans W. C. Oxidative metabolism of naphthalene by soil pseudomonads. The ring-fission mechanism. Biochem J. 1964 May;91(2):251–261. doi: 10.1042/bj0910251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Efroymson R. A., Alexander M. Biodegradation by an arthrobacter species of hydrocarbons partitioned into an organic solvent. Appl Environ Microbiol. 1991 May;57(5):1441–1447. doi: 10.1128/aem.57.5.1441-1447.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Foght J. M., Gutnick D. L., Westlake D. W. Effect of emulsan on biodegradation of crude oil by pure and mixed bacterial cultures. Appl Environ Microbiol. 1989 Jan;55(1):36–42. doi: 10.1128/aem.55.1.36-42.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guerin W. F., Jones G. E. Mineralization of phenanthrene by a Mycobacterium sp. Appl Environ Microbiol. 1988 Apr;54(4):937–944. doi: 10.1128/aem.54.4.937-944.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller R. M., Bartha R. Evidence from liposome encapsulation for transport-limited microbial metabolism of solid alkanes. Appl Environ Microbiol. 1989 Feb;55(2):269–274. doi: 10.1128/aem.55.2.269-274.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mulkins-Phillips G. J., Stewart J. E. Effect of four dispersants on biodegradation and growth of bacteria on crude oil. Appl Microbiol. 1974 Oct;28(4):547–552. doi: 10.1128/am.28.4.547-552.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oberbremer A., Müller-Hurtig R., Wagner F. Effect of the addition of microbial surfactants on hydrocarbon degradation in a soil population in a stirred reactor. Appl Microbiol Biotechnol. 1990 Jan;32(4):485–489. doi: 10.1007/BF00903788. [DOI] [PubMed] [Google Scholar]