Abstract
The Aspergillus terreus gla1 gene, coding for a glucoamylase, has been cloned by heterologous hybridization. The gene is interrupted by four introns and encodes a protein with an N-terminal catalytic domain and a C-terminal starch-binding domain. The expression of the gene is induced by starch and maltose and repressed by glucose.
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Selected References
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- Bailey C., Arst H. N., Jr Carbon catabolite repression in Aspergillos nidulans. Eur J Biochem. 1975 Feb 21;51(2):573–577. doi: 10.1111/j.1432-1033.1975.tb03958.x. [DOI] [PubMed] [Google Scholar]
- Bhella R. S., Altosaar I. Role of cAMP in the mediation of glucose catabolite repression of glucoamylase synthesis in Aspergillus awamori. Curr Genet. 1988 Sep;14(3):247–252. doi: 10.1007/BF00376745. [DOI] [PubMed] [Google Scholar]
- Boel E., Hansen M. T., Hjort I., Høegh I., Fiil N. P. Two different types of intervening sequences in the glucoamylase gene from Aspergillus niger. EMBO J. 1984 Jul;3(7):1581–1585. doi: 10.1002/j.1460-2075.1984.tb02014.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boel E., Hjort I., Svensson B., Norris F., Norris K. E., Fiil N. P. Glucoamylases G1 and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs. EMBO J. 1984 May;3(5):1097–1102. doi: 10.1002/j.1460-2075.1984.tb01935.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cubero B., Scazzocchio C. Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J. 1994 Jan 15;13(2):407–415. doi: 10.1002/j.1460-2075.1994.tb06275.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donnet A., Dessi P., Ali Cherif A. Le syndrome idiopathique de paralysies multiples et récurrentes des nerfs crâniens. Ann Med Interne (Paris) 1991;142(2):144–146. [PubMed] [Google Scholar]
- Fowler T., Berka R. M., Ward M. Regulation of the glaA gene of Aspergillus niger. Curr Genet. 1990 Dec;18(6):537–545. doi: 10.1007/BF00327025. [DOI] [PubMed] [Google Scholar]
- Ghose A., Chatterjee B. S., Das A. Characterization of glucoamylase from Aspergillus terreus 4. FEMS Microbiol Lett. 1990 Jan 1;54(1-3):345–349. doi: 10.1111/j.1574-6968.1990.tb04024.x. [DOI] [PubMed] [Google Scholar]
- Hata Y., Kitamoto K., Gomi K., Kumagai C., Tamura G. Functional elements of the promoter region of the Aspergillus oryzae glaA gene encoding glucoamylase. Curr Genet. 1992 Aug;22(2):85–91. doi: 10.1007/BF00351466. [DOI] [PubMed] [Google Scholar]
- Hata Y., Tsuchiya K., Kitamoto K., Gomi K., Kumagai C., Tamura G., Hara S. Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA) from Aspergillus oryzae. Gene. 1991 Dec 1;108(1):145–150. doi: 10.1016/0378-1119(91)90500-b. [DOI] [PubMed] [Google Scholar]
- Hintz W. E., Lagosky P. A. A glucose-derepressed promoter for expression of heterologous products in the filamentous fungus Aspergillus nidulans. Biotechnology (N Y) 1993 Jul;11(7):815–818. doi: 10.1038/nbt0793-815. [DOI] [PubMed] [Google Scholar]
- Joutsjoki V. V., Torkkeli T. K. Glucoamylase P gene of Hormoconis resinae: molecular cloning, sequencing and introduction into Trichoderma reesei. FEMS Microbiol Lett. 1992 Dec 1;78(2-3):237–243. doi: 10.1016/0378-1097(92)90033-k. [DOI] [PubMed] [Google Scholar]
- Kulmburg P., Mathieu M., Dowzer C., Kelly J., Felenbok B. Specific binding sites in the alcR and alcA promoters of the ethanol regulon for the CREA repressor mediating carbon catabolite repression in Aspergillus nidulans. Mol Microbiol. 1993 Mar;7(6):847–857. doi: 10.1111/j.1365-2958.1993.tb01175.x. [DOI] [PubMed] [Google Scholar]
- Nunberg J. H., Meade J. H., Cole G., Lawyer F. C., McCabe P., Schweickart V., Tal R., Wittman V. P., Flatgaard J. E., Innis M. A. Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori. Mol Cell Biol. 1984 Nov;4(11):2306–2315. doi: 10.1128/mcb.4.11.2306. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramón D., Carramolino L., Patiño C., Sánchez F., Peñalva M. A. Cloning and characterization of the isopenicillin N synthetase gene mediating the formation of the beta-lactam ring in Aspergillus nidulans. Gene. 1987;57(2-3):171–181. doi: 10.1016/0378-1119(87)90120-x. [DOI] [PubMed] [Google Scholar]
- Shibuya I., Gomi K., Iimura Y., Takahashi K., Tamura G., Hara S. Molecular cloning of the glucoamylase gene of Aspergillus shirousami and its expression in Aspergillus oryzae. Agric Biol Chem. 1990 Aug;54(8):1905–1914. [PubMed] [Google Scholar]
- Sierks M. R., Ford C., Reilly P. J., Svensson B. Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori. Protein Eng. 1990 Jan;3(3):193–198. doi: 10.1093/protein/3.3.193. [DOI] [PubMed] [Google Scholar]
- Sierks M. R., Ford C., Reilly P. J., Svensson B. Site-directed mutagenesis at the active site Trp120 of Aspergillus awamori glucoamylase. Protein Eng. 1989 Aug;2(8):621–625. doi: 10.1093/protein/2.8.621. [DOI] [PubMed] [Google Scholar]
- Stone P. J., Makoff A. J., Parish J. H., Radford A. Cloning and sequence analysis of the glucoamylase gene of Neurospora crassa. Curr Genet. 1993 Sep;24(3):205–211. doi: 10.1007/BF00351793. [DOI] [PubMed] [Google Scholar]
- Ventura L., Ramón D., Pérez-González J. A. Isolation of an Aspergillus terreus mutant impaired in arginine biosynthesis and its complementation with the argB gene from Aspergillus nidulans. FEMS Microbiol Lett. 1992 Dec 1;78(2-3):187–191. doi: 10.1016/0378-1097(92)90023-h. [DOI] [PubMed] [Google Scholar]
- Ventura L., Ramón D. Transformation of Aspergillus terreus with the hygromycin B resistance marker from Escherichia coli. FEMS Microbiol Lett. 1991 Aug 1;66(2):189–193. doi: 10.1016/0378-1097(91)90331-4. [DOI] [PubMed] [Google Scholar]
