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. 1995 Feb;61(2):501–506. doi: 10.1128/aem.61.2.501-506.1995

Bacteriostatic effect of orally administered bovine lactoferrin on proliferation of Clostridium species in the gut of mice fed bovine milk.

S Teraguchi 1, K Shin 1, K Ozawa 1, S Nakamura 1, Y Fukuwatari 1, S Tsuyuki 1, H Namihira 1, S Shimamura 1
PMCID: PMC167309  PMID: 7574587

Abstract

When milk-fed mice were orally inoculated with Clostridium ramosum C1, this strain proliferated in the gut and became the dominant component of the fecal microflora. In this experimental model, bovine lactoferrin (bLF) administered with milk suppressed the proliferation of this strain in vivo and decreased the numbers of C. ramosum and other bacteria in the feces. This bacteriostatic effect of bLF was dependent on the concentration of bLF, the duration of feeding, and the administered dose of C. ramosum C1. Compared with bovine serum albumin, ovalbumin, bovine whey protein isolate, or bovine casein, only bLF showed this specific activity. A similar effect of bLF was observed after oral inoculation with C. ramosum JCM 1298, C. paraputrificum VPI 6372, or C. perfringens ATCC 13124. A hydrolysate prepared by digestion of bLF with porcine pepsin showed the same inhibitory effect on proliferation of C. ramosum in vivo as occurred with undigested bLF. These results indicate that ingested bLF can exert a bacteriostatic effect against clostridia in the gut even after it has been digested to some extent.

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Selected References

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  1. Bellamy W., Takase M., Wakabayashi H., Kawase K., Tomita M. Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. J Appl Bacteriol. 1992 Dec;73(6):472–479. doi: 10.1111/j.1365-2672.1992.tb05007.x. [DOI] [PubMed] [Google Scholar]
  2. Bellamy W., Takase M., Yamauchi K., Wakabayashi H., Kawase K., Tomita M. Identification of the bactericidal domain of lactoferrin. Biochim Biophys Acta. 1992 May 22;1121(1-2):130–136. doi: 10.1016/0167-4838(92)90346-f. [DOI] [PubMed] [Google Scholar]
  3. Benno Y., Suzuki K., Suzuki K., Narisawa K., Bruce W. R., Mitsuoka T. Comparison of the fecal microflora in rural Japanese and urban Canadians. Microbiol Immunol. 1986;30(6):521–532. doi: 10.1111/j.1348-0421.1986.tb02978.x. [DOI] [PubMed] [Google Scholar]
  4. Brock J. H. Lactoferrin in human milk: its role in iron absorption and protection against enteric infection in the newborn infant. Arch Dis Child. 1980 Jun;55(6):417–421. doi: 10.1136/adc.55.6.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bullen J. J. Iron-binding proteins in milk and resistance to Escherichia coli infection in infants. Postgrad Med J. 1975;51 Suppl 3:67–70. [PubMed] [Google Scholar]
  6. Bullen J. J. The significance of iron in infection. Rev Infect Dis. 1981 Nov-Dec;3(6):1127–1138. doi: 10.1093/clinids/3.6.1127. [DOI] [PubMed] [Google Scholar]
  7. France G. L., Marmer D. J., Steele R. W. Breast-feeding and Salmonella infection. Am J Dis Child. 1980 Feb;134(2):147–152. doi: 10.1001/archpedi.1980.02130140021007. [DOI] [PubMed] [Google Scholar]
  8. Gunn R. A., Kimball A. M., Pollard R. A., Feeley J. C., Feldman R. A., Dutta S. R., Matthew P. P., Mahmood R. A., Levine M. M. Bottle feeding as a risk factor for cholera in infants. Lancet. 1979 Oct 6;2(8145):730–732. doi: 10.1016/s0140-6736(79)90653-6. [DOI] [PubMed] [Google Scholar]
  9. Howie P. W., Forsyth J. S., Ogston S. A., Clark A., Florey C. D. Protective effect of breast feeding against infection. BMJ. 1990 Jan 6;300(6716):11–16. doi: 10.1136/bmj.300.6716.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mitsuoka T., Kaneuchi C. Ecology of the bifidobacteria. Am J Clin Nutr. 1977 Nov;30(11):1799–1810. doi: 10.1093/ajcn/30.11.1799. [DOI] [PubMed] [Google Scholar]
  11. Mitsuoka T., Ohno K., Benno Y., Suzuki K., Namba K. Die Faekalflora bei Menschen. IV. Mitteilung: Vergleich des neu entwickelten Verfahrens mit dem bisherigen üblichen Verfahren zur Darmfloraanalyse. Zentralbl Bakteriol Orig A. 1976 Mar;234(2):219–233. [PubMed] [Google Scholar]
  12. Mitsuoka T., Sega T., Yamamoto S. Eine verbesserte Methodik der qualitativen und quantitativen Analyse der Darmflora von Menschen und Tieren. Zentralbl Bakteriol Orig. 1965 Mar;195(4):455–469. [PubMed] [Google Scholar]
  13. Sánchez L., Calvo M., Brock J. H. Biological role of lactoferrin. Arch Dis Child. 1992 May;67(5):657–661. doi: 10.1136/adc.67.5.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tomita M., Bellamy W., Takase M., Yamauchi K., Wakabayashi H., Kawase K. Potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin. J Dairy Sci. 1991 Dec;74(12):4137–4142. doi: 10.3168/jds.S0022-0302(91)78608-6. [DOI] [PubMed] [Google Scholar]

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