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. 1995 Feb;61(2):807–810. doi: 10.1128/aem.61.2.807-810.1995

Nonradioactive colony hybridization assay for detection and enumeration of enterotoxigenic Clostridium perfringens in raw beef.

L A Baez 1, V K Juneja 1
PMCID: PMC167342  PMID: 7574619

Abstract

A DNA probe endolabeled with digoxigenin by PCR was developed to detect and enumerate enterotoxigenic Clostridium perfringens in raw beef. After 2 h of hybridization, membranes were developed by using an anti-digoxigenin-alkaline phosphatase conjugated antibody. The resulting chromogenic reaction allowed us to detect and enumerate < or = 10 CFU of C. perfringens per g.

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Selected References

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