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. 1995 Feb;61(2):817–819. doi: 10.1128/aem.61.2.817-819.1995

Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers.

L M Herman 1, J H De Block 1, R J Moermans 1
PMCID: PMC167343  PMID: 7574620

Abstract

A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.

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Selected References

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