Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1995 Feb;61(2):820–822. doi: 10.1128/aem.61.2.820-822.1995

Possible errors in assay for beta-glycosidase activity.

R W Chadwick 1, J C Allison 1, D L Talley 1, S E George 1
PMCID: PMC167344  PMID: 7574621

Abstract

Cecal homogenates were assayed for the enzymes beta-glucosidase, beta-glucuronidase, and beta-galactosidase. Anaerobic incubation with the addition of excess 3,4-dichloronitrobenzene, a substrate for nitroreductase, significantly increased the detection of the beta-glycosidase enzymes' activities.

Full Text

The Full Text of this article is available as a PDF (160.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bokkenheuser V. D., Shackleton C. H., Winter J. Hydrolysis of dietary flavonoid glycosides by strains of intestinal Bacteroides from humans. Biochem J. 1987 Dec 15;248(3):953–956. doi: 10.1042/bj2480953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Celik C., Lewis D. A., Mittleman A. Induction of colon mucosal beta-glucuronidase production as a mechanism for 1,2-dimethylhydrazine colon carcinogenesis. J Surg Oncol. 1983 Nov;24(3):209–211. doi: 10.1002/jso.2930240313. [DOI] [PubMed] [Google Scholar]
  3. Chadwick R. W., George S. E., Claxton L. D. Role of the gastrointestinal mucosa and microflora in the bioactivation of dietary and environmental mutagens or carcinogens. Drug Metab Rev. 1992;24(4):425–492. doi: 10.3109/03602539208996302. [DOI] [PubMed] [Google Scholar]
  4. Goldin B. R. In situ bacterial metabolism and colon mutagens. Annu Rev Microbiol. 1986;40:367–393. doi: 10.1146/annurev.mi.40.100186.002055. [DOI] [PubMed] [Google Scholar]
  5. Hawksworth G., Drasar B. S., Hill M. J. Intestinal bacteria and the hydrolysis of glycosidic bonds. J Med Microbiol. 1971 Nov;4(4):451–459. doi: 10.1099/00222615-4-4-451. [DOI] [PubMed] [Google Scholar]
  6. MacDonald I. A., Bussard R. G., Hutchison D. M., Holdeman L. V. Rutin-induced beta-glucosidase activity in Streptococcus faecium VGH-1 and Streptococcus sp. strain FRP-17 isolated from human feces: formation of the mutagen, quercetin, from rutin. Appl Environ Microbiol. 1984 Feb;47(2):350–355. doi: 10.1128/aem.47.2.350-355.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mason R. P., Holtzman J. L. The role of catalytic superoxide formation in the O2 inhibition of nitroreductase. Biochem Biophys Res Commun. 1975 Dec 15;67(4):1267–1274. doi: 10.1016/0006-291x(75)90163-1. [DOI] [PubMed] [Google Scholar]
  8. Morotomi M., Nanno M., Watanabe T., Sakurai T., Mutai M. Mutagenic activation of biliary metabolites of 1-nitropyrene by intestinal microflora. Mutat Res. 1985 Apr;149(2):171–178. doi: 10.1016/0027-5107(85)90023-5. [DOI] [PubMed] [Google Scholar]
  9. Rowland I. R., Wise A., Mallett A. K. Metabolic profile of caecal micro-organisms from rats fed indigestible plant cell-wall components. Food Chem Toxicol. 1983 Feb;21(1):25–29. doi: 10.1016/0278-6915(83)90264-8. [DOI] [PubMed] [Google Scholar]
  10. Sarhan H. R., Foster H. A. A rapid fluorogenic method for the detection of Escherichia coli by the production of beta-glucuronidase. J Appl Bacteriol. 1991 May;70(5):394–400. doi: 10.1111/j.1365-2672.1991.tb02955.x. [DOI] [PubMed] [Google Scholar]
  11. Schulze J., Herzog R., Müller-Beuthow W., Grütte F. K. Intestinale beta-Galactosidase-Aktivität im gnotobiotischen Tier nachMonoassoziation mit verschiedenen E. coli-Stämmen. Nahrung. 1987;31(5-6):625–629. doi: 10.1002/food.19870310582. [DOI] [PubMed] [Google Scholar]
  12. Weisburger J. H., Grantham P. H., Horton R. E., Weisburger E. K. Metabolism of the carcinogen N-hydroxy-N-2-fluorenylacetamide in germ-free rats. Biochem Pharmacol. 1970 Jan;19(1):151–162. doi: 10.1016/0006-2952(70)90336-9. [DOI] [PubMed] [Google Scholar]
  13. Williams J. R., Jr, Grantham P. H., Marsh H. H., 3rd, Weisburger J. H., Weisburger E. K. Participation of liver fractions and of intestinal bacteria in the metabolism of N-hydroxy-N-2-fluorenylacetamide in the rat. Biochem Pharmacol. 1970 Jan;19(1):173–188. doi: 10.1016/0006-2952(70)90338-2. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES