Abstract
The significance of bile salt hydrolase production by lactobacilli in the microecology of the murine intestinal tract has not been extensively studied previously. Assays of bile salt hydrolase (sodium taurocholate as substrate) associated with cell extracts of five Lactobacillus strains of murine origin gave a range of activities (from 915 nmol of cholate released per mg of protein per 30 min to none detected). All of the strains tested colonized the murine gastrointestinal tract equally well. The growth rates of mice were not affected by colonization of their intestinal tracts by lactobacilli whether or not the bacteria produced bile salt hydrolase.
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- Dashkevicz M. P., Feighner S. D. Development of a differential medium for bile salt hydrolase-active Lactobacillus spp. Appl Environ Microbiol. 1989 Jan;55(1):11–16. doi: 10.1128/aem.55.1.11-16.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feighner S. D., Dashkevicz M. P. Effect of dietary carbohydrates on bacterial cholyltaurine hydrolase in poultry intestinal homogenates. Appl Environ Microbiol. 1988 Feb;54(2):337–342. doi: 10.1128/aem.54.2.337-342.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feighner S. D., Dashkevicz M. P. Subtherapeutic levels of antibiotics in poultry feeds and their effects on weight gain, feed efficiency, and bacterial cholyltaurine hydrolase activity. Appl Environ Microbiol. 1987 Feb;53(2):331–336. doi: 10.1128/aem.53.2.331-336.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lundeen S. G., Savage D. C. Characterization of an extracellular factor that stimulates bile salt hydrolase activity in Lactobacillus sp. strain 100-100. FEMS Microbiol Lett. 1992 Jul 1;73(1-2):121–126. doi: 10.1016/0378-1097(92)90594-e. [DOI] [PubMed] [Google Scholar]
- SCHAEDLER R. W., DUBOS R. J. The fecal flora of various strains of mice. Its bearing on their susceptibility to endotoxin. J Exp Med. 1962 Jun 1;115:1149–1160. doi: 10.1084/jem.115.6.1149. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tannock G. W., Archibald R. D. The derivation and use of mice which do not harbour lactobacilli in the gastrointestinal tract. Can J Microbiol. 1984 Jun;30(6):849–853. doi: 10.1139/m84-131. [DOI] [PubMed] [Google Scholar]
- Tannock G. W., Crichton C., Welling G. W., Koopman J. P., Midtvedt T. Reconstitution of the gastrointestinal microflora of lactobacillus-free mice. Appl Environ Microbiol. 1988 Dec;54(12):2971–2975. doi: 10.1128/aem.54.12.2971-2975.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tannock G. W., Dashkevicz M. P., Feighner S. D. Lactobacilli and bile salt hydrolase in the murine intestinal tract. Appl Environ Microbiol. 1989 Jul;55(7):1848–1851. doi: 10.1128/aem.55.7.1848-1851.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tannock G. W., Savage D. C. Influences of dietary and environmental stress on microbial populations in the murine gastrointestinal tract. Infect Immun. 1974 Mar;9(3):591–598. doi: 10.1128/iai.9.3.591-598.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tannock G. W., Tangerman A., Van Schaik A., McConnell M. A. Deconjugation of bile acids by lactobacilli in the mouse small bowel. Appl Environ Microbiol. 1994 Sep;60(9):3419–3420. doi: 10.1128/aem.60.9.3419-3420.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]