Abstract
Eleven strains of Aeromonas salmonicida were passaged twice by intraperitoneal injection through rainbow trout and reisolated from the kidney of moribund fish. The surface characteristics and virulence of the strains changed following passage through fish. None of the in vitro tests used could effectively predict the in vivo virulence.
Full Text
The Full Text of this article is available as a PDF (158.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Atkinson H. M., Trust T. J. Hemagglutination properties and adherence ability of Aeromonas hydrophila. Infect Immun. 1980 Mar;27(3):938–946. doi: 10.1128/iai.27.3.938-946.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crosa J. H., Hodges L. L. Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum 775. Infect Immun. 1981 Jan;31(1):223–227. doi: 10.1128/iai.31.1.223-227.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evenberg D., Versluis R., Lugtenberg B. Biochemical and immunological characterization of the cell surface of the fish pathogenic bacterium Aeromonas salmonicida. Biochim Biophys Acta. 1985 May 14;815(2):233–244. doi: 10.1016/0005-2736(85)90294-9. [DOI] [PubMed] [Google Scholar]
- Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janda J. M., Oshiro L. S., Abbott S. L., Duffey P. S. Virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer. Infect Immun. 1987 Dec;55(12):3070–3077. doi: 10.1128/iai.55.12.3070-3077.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kay W. W., Buckley J. T., Ishiguro E. E., Phipps B. M., Monette J. P., Trust T. J. Purification and disposition of a surface protein associated with virulence of Aeromonas salmonicida. J Bacteriol. 1981 Sep;147(3):1077–1084. doi: 10.1128/jb.147.3.1077-1084.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lindahl M., Faris A., Wadström T., Hjertén S. A new test based on 'salting out' to measure relative surface hydrophobicity of bacterial cells. Biochim Biophys Acta. 1981 Nov 5;677(3-4):471–476. doi: 10.1016/0304-4165(81)90261-0. [DOI] [PubMed] [Google Scholar]
- Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
- Statner B., Jones M. J., George W. L. Effect of incubation temperature on growth and soluble protein profiles of motile Aeromonas strains. J Clin Microbiol. 1988 Feb;26(2):392–393. doi: 10.1128/jcm.26.2.392-393.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
- Van Alstine J. M., Trust T. J., Brooks D. E. Differential partition of virulent Aeromonas salmonicida and attenuated derivatives possessing specific cell surface alterations in polymer aqueous-phase systems. Appl Environ Microbiol. 1986 Jun;51(6):1309–1313. doi: 10.1128/aem.51.6.1309-1313.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]