Abstract
A number of microorganisms were selected from soil and sediment samples which were known to have been previously exposed to nitrate ester contaminants. The two most effective bacteria for transforming glycerol trinitrate (GTN) were identified as Bacillus thuringiensis/cereus and Enterobacter agglomerans. For both isolates, denitration activities were expressed constitutively and GTN was not required for induction. Dialysis of cell extracts from both isolates did not affect denitration, which indicates that dissociable and depletable cofactors are not required for denitration. With thin-layer chromatography and high-performance liquid chromatography, the denitration pathway for both isolates was shown to be a sequential denitration of GTN to glycerol dinitrate isomers, glycerol mononitrate isomers, and ultimately to glycerol. GTN was observed to be completely converted to glycerol during a long-term incubation of cell extracts.
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- Coppella S. J., DelaCruz N., Payne G. F., Pogell B. M., Speedie M. K., Karns J. S., Sybert E. M., Connor M. A. Genetic engineering approach to toxic waste management: case study for organophosphate waste treatment. Biotechnol Prog. 1990 Jan-Feb;6(1):76–81. doi: 10.1021/bp00001a012. [DOI] [PubMed] [Google Scholar]
- Ducrocq C., Servy C., Lenfant M. Bioconversion of glyceryl trinitrate into mononitrates by Geotrichum candidum. FEMS Microbiol Lett. 1989 Nov;53(1-2):219–222. doi: 10.1016/0378-1097(89)90394-7. [DOI] [PubMed] [Google Scholar]
- Ducrocq C., Servy C., Lenfant M. Formation of glyceryl 2-mononitrate by regioselective bioconversion of glyceryl trinitrate: efficiency of the filamentous fungus Phanerochaete chrysosporium. Biotechnol Appl Biochem. 1990 Jun;12(3):325–330. [PubMed] [Google Scholar]
- Kaplan D. L., Cornell J. H., Kaplan A. M. Biodegradation of glycidol and glycidyl nitrate. Appl Environ Microbiol. 1982 Jan;43(1):144–150. doi: 10.1128/aem.43.1.144-150.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Servent D., Ducrocq C., Henry Y., Guissani A., Lenfant M. Nitroglycerin metabolism by Phanerochaete chrysosporium: evidence for nitric oxide and nitrite formation. Biochim Biophys Acta. 1991 Jul 8;1074(2):320–325. doi: 10.1016/0304-4165(91)90170-l. [DOI] [PubMed] [Google Scholar]
- Servent D., Ducrocq C., Henry Y., Servy C., Lenfant M. Multiple enzymatic pathways involved in the metabolism of glyceryl trinitrate in Phanerochaete chrysosporium. Biotechnol Appl Biochem. 1992 Jun;15(3):257–266. [PubMed] [Google Scholar]
- Wendt T. M., Cornell J. H., Kaplan A. M. Microbial degradation of glycerol nitrates. Appl Environ Microbiol. 1978 Nov;36(5):693–699. doi: 10.1128/aem.36.5.693-699.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White G. F., Snape J. R. Microbial cleavage of nitrate esters: defusing the environment. J Gen Microbiol. 1993 Sep;139(9):1947–1957. doi: 10.1099/00221287-139-9-1947. [DOI] [PubMed] [Google Scholar]
- Wyman J. F., Guard H. E., Coleman W. M., 3rd Environmental chemistry of 1,2-propanediol dinitrate: azeotrope formation, photolysis and biodegradability. Arch Environ Contam Toxicol. 1984 Nov;13(6):647–652. doi: 10.1007/BF01055927. [DOI] [PubMed] [Google Scholar]