Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1995 Jul;61(7):2759–2761. doi: 10.1128/aem.61.7.2759-2761.1995

DNA fingerprinting by pulsed-field gel electrophoresis to investigate a nosocomial pneumonia caused by Legionella bozemanii serogroup 1.

P C Lück 1, J H Helbig 1, H J Hagedorn 1, W Ehret 1
PMCID: PMC167548  PMID: 7618888

Abstract

We typed 18 isolates of Legionella bozemanii obtained from clinical and environmental sources by pulsed-field gel electrophoresis. Each of the unrelated strains showed individual restriction patterns of the genomic DNA when either the SfiI or NotI restriction enzyme was used. One strain isolated from a patient with nosocomial legionellosis and two strains from the corresponding hospital water supply were indistinguishable, arguing for a transmission of L. bozemanii from the water supply to the patient. In conclusion, macrorestriction analysis is a valuable tool for studies of the molecular epidemiology of L. bozemanii.

Full Text

The Full Text of this article is available as a PDF (296.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fang G. D., Yu V. L., Vickers R. M. Disease due to the Legionellaceae (other than Legionella pneumophila). Historical, microbiological, clinical, and epidemiological review. Medicine (Baltimore) 1989 Mar;68(2):116–132. doi: 10.1097/00005792-198903000-00005. [DOI] [PubMed] [Google Scholar]
  2. Korvick J. A., Yu V. L., Fang G. D. Legionella species as hospital-acquired respiratory pathogens. Semin Respir Infect. 1987 Mar;2(1):34–47. [PubMed] [Google Scholar]
  3. Lode H., Kemmerich B., Schäfer H., Grothe R., Hartmann R., Ehret W., Ruckdeschel G. Significance of non-pneumophila Legionella species in adult community-acquired and nosocomial pneumonias. Klin Wochenschr. 1987 May 15;65(10):463–468. doi: 10.1007/BF01712839. [DOI] [PubMed] [Google Scholar]
  4. Lück P. C., Bender L., Ott M., Helbig J. H., Hacker J. Analysis of Legionella pneumophila serogroup 6 strains isolated from a hospital warm water supply over a three-year period by using genomic long-range mapping techniques and monoclonal antibodies. Appl Environ Microbiol. 1991 Nov;57(11):3226–3231. doi: 10.1128/aem.57.11.3226-3231.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lück P. C., Helbig J. H., Günter U., Assmann M., Blau R., Koch H., Klepp M. Epidemiologic investigation by macrorestriction analysis and by using monoclonal antibodies of nosocomial pneumonia caused by Legionella pneumophila serogroup 10. J Clin Microbiol. 1994 Nov;32(11):2692–2697. doi: 10.1128/jcm.32.11.2692-2697.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ott M., Bender L., Marre R., Hacker J. Pulsed field electrophoresis of genomic restriction fragments for the detection of nosocomial Legionella pneumophila in hospital water supplies. J Clin Microbiol. 1991 Apr;29(4):813–815. doi: 10.1128/jcm.29.4.813-815.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Parry M. F., Stampleman L., Hutchinson J. H., Folta D., Steinberg M. G., Krasnogor L. J. Waterborne Legionella bozemanii and nosocomial pneumonia in immunosuppressed patients. Ann Intern Med. 1985 Aug;103(2):205–210. doi: 10.7326/0003-4819-103-2-205. [DOI] [PubMed] [Google Scholar]
  8. Schoonmaker D., Heimberger T., Birkhead G. Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophila isolates obtained during a nosocomial outbreak. J Clin Microbiol. 1992 Jun;30(6):1491–1498. doi: 10.1128/jcm.30.6.1491-1498.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tompkins L. S., Troup N. J., Woods T., Bibb W., McKinney R. M. Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis. J Clin Microbiol. 1987 Oct;25(10):1875–1880. doi: 10.1128/jcm.25.10.1875-1880.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Woods T. C., McKinney R. M., Plikaytis B. D., Steigerwalt A. G., Bibb W. F., Brenner D. J. Multilocus enzyme analysis of Legionella dumoffii. J Clin Microbiol. 1988 May;26(5):799–803. doi: 10.1128/jcm.26.5.799-803.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES