Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1995 Aug;61(8):3172–3176. doi: 10.1128/aem.61.8.3172-3176.1995

Characterization of plasmid-encoded citrate permease (citP) genes from Leuconostoc species reveals high sequence conservation with the Lactococcus lactis citP gene.

E E Vaughan 1, S David 1, A Harrington 1, C Daly 1, G F Fitzgerald 1, W M De Vos 1
PMCID: PMC167593  PMID: 7487049

Abstract

The citrate permease determinant (citP) in several Leuconostoc strains was demonstrated to be plasmid encoded by curing experiments and hybridization studies with a DNA fragment containing the citP gene from Lactococcus lactis subsp. lactis biovar diacetylactis NCDO176. Cloning and nucleotide sequence analysis of Leuconostoc lactis NZ6070 citP revealed almost complete identity to lactococcal citP.

Full Text

The Full Text of this article is available as a PDF (342.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  3. Coffey A., Harrington A., Kearney K., Daly C., Fitzgerald G. Nucleotide sequence and structural organization of the small, broad-host-range plasmid pCI411 from Leuconostoc lactis 533. Microbiology. 1994 Sep;140(Pt 9):2263–2269. doi: 10.1099/13500872-140-9-2263. [DOI] [PubMed] [Google Scholar]
  4. Daimon H., Ishiguro N., Shinagawa M., Sato G. Nucleotide sequence of a citrate utilization gene from Citrobacter amalonaticus. J Bacteriol. 1989 Jan;171(1):620–623. doi: 10.1128/jb.171.1.620-623.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. David S., Simons G., De Vos W. M. Plasmid transformation by electroporation of Leuconostoc paramesenteroides and its use in molecular cloning. Appl Environ Microbiol. 1989 Jun;55(6):1483–1489. doi: 10.1128/aem.55.6.1483-1489.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. David S., van der Rest M. E., Driessen A. J., Simons G., de Vos W. M. Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene. J Bacteriol. 1990 Oct;172(10):5789–5794. doi: 10.1128/jb.172.10.5789-5794.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dimroth P., Thomer A. Citrate transport in Klebsiella pneumoniae. Biol Chem Hoppe Seyler. 1986 Aug;367(8):813–823. doi: 10.1515/bchm3.1986.367.2.813. [DOI] [PubMed] [Google Scholar]
  8. Hastings J. W., Sailer M., Johnson K., Roy K. L., Vederas J. C., Stiles M. E. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J Bacteriol. 1991 Dec;173(23):7491–7500. doi: 10.1128/jb.173.23.7491-7500.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hastings J. W., Stiles M. E. Antibiosis of Leuconostoc gelidum isolated from meat. J Appl Bacteriol. 1991 Feb;70(2):127–134. doi: 10.1111/j.1365-2672.1991.tb04438.x. [DOI] [PubMed] [Google Scholar]
  10. Hugenholtz J., Perdon L., Abee T. Growth and Energy Generation by Lactococcus lactis subsp. lactis biovar diacetylactis during Citrate Metabolism. Appl Environ Microbiol. 1993 Dec;59(12):4216–4222. doi: 10.1128/aem.59.12.4216-4222.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ishiguro N., Izawa H., Shinagawa M., Shimamoto T., Tsuchiya T. Cloning and nucleotide sequence of the gene (citC) encoding a citrate carrier from several Salmonella serovars. J Biol Chem. 1992 May 15;267(14):9559–9564. [PubMed] [Google Scholar]
  12. Ishiguro N., Sato G. Nucleotide sequence of the gene determining plasmid-mediated citrate utilization. J Bacteriol. 1985 Dec;164(3):977–982. doi: 10.1128/jb.164.3.977-982.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jahns A., Schäfer A., Geis A., Teuber M. Identification, cloning and sequencing of the replication region of Lactococcus lactis ssp. lactis biovar. diacetylactis Bu2 citrate plasmid pSL2. FEMS Microbiol Lett. 1991 May 15;64(2-3):253–258. doi: 10.1016/0378-1097(91)90605-a. [DOI] [PubMed] [Google Scholar]
  14. Kempler G. M., McKay L. L. Characterization of Plasmid Deoxyribonucleic Acid in Streptococcus lactis subsp. diacetylactis: Evidence for Plasmid-Linked Citrate Utilization. Appl Environ Microbiol. 1979 Feb;37(2):316–323. doi: 10.1128/aem.37.2.316-323.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lolkema J. S., Enequist H., van der Rest M. E. Transport of citrate catalyzed by the sodium-dependent citrate carrier of Klebsiella pneumoniae is obligatorily coupled to the transport of two sodium ions. Eur J Biochem. 1994 Mar 1;220(2):469–475. doi: 10.1111/j.1432-1033.1994.tb18645.x. [DOI] [PubMed] [Google Scholar]
  16. López de Felipe F., Magni C., de Mendoza D., López P. Citrate utilization gene cluster of the Lactococcus lactis biovar diacetylactis: organization and regulation of expression. Mol Gen Genet. 1995 Mar 10;246(5):590–599. doi: 10.1007/BF00298965. [DOI] [PubMed] [Google Scholar]
  17. Orberg P. K., Sandine W. E. Common occurrence of plasmid DNA and vancomycin resistance in Leuconostoc spp. Appl Environ Microbiol. 1984 Dec;48(6):1129–1133. doi: 10.1128/aem.48.6.1129-1133.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ramos A., Poolman B., Santos H., Lolkema J. S., Konings W. N. Uniport of anionic citrate and proton consumption in citrate metabolism generates a proton motive force in Leuconostoc oenos. J Bacteriol. 1994 Aug;176(16):4899–4905. doi: 10.1128/jb.176.16.4899-4905.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reddy M. S., Vedamuthu E. R., Washam C. J., Reinbold G. W. Agar medium for differential enumeration of lactic streptococci. Appl Microbiol. 1972 Dec;24(6):947–952. doi: 10.1128/am.24.6.947-952.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sasatsu M., Misra T. K., Chu L., Laddaga R., Silver S. Cloning and DNA sequence of a plasmid-determined citrate utilization system in Escherichia coli. J Bacteriol. 1985 Dec;164(3):983–993. doi: 10.1128/jb.164.3.983-993.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schwarz E., Oesterhelt D. Cloning and expression of Klebsiella pneumoniae genes coding for citrate transport and fermentation. EMBO J. 1985 Jun;4(6):1599–1603. doi: 10.1002/j.1460-2075.1985.tb03823.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sesma F., Gardiol D., de Ruiz Holgado A. P., de Mendoza D. Cloning of the citrate permease gene of Lactococcus lactis subsp. lactis biovar diacetylactis and expression in Escherichia coli. Appl Environ Microbiol. 1990 Jul;56(7):2099–2103. doi: 10.1128/aem.56.7.2099-2103.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shimamoto T., Izawa H., Daimon H., Ishiguro N., Shinagawa M., Sakano Y., Tsuda M., Tsuchiya T. Cloning and nucleotide sequence of the gene (citA) encoding a citrate carrier from Salmonella typhimurium. J Biochem. 1991 Jul;110(1):22–28. doi: 10.1093/oxfordjournals.jbchem.a123537. [DOI] [PubMed] [Google Scholar]
  25. Twigg A. J., Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980 Jan 10;283(5743):216–218. doi: 10.1038/283216a0. [DOI] [PubMed] [Google Scholar]
  26. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  27. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  28. de Vos W. M., Vaughan E. E. Genetics of lactose utilization in lactic acid bacteria. FEMS Microbiol Rev. 1994 Oct;15(2-3):217–237. doi: 10.1111/j.1574-6976.1994.tb00136.x. [DOI] [PubMed] [Google Scholar]
  29. van der Rest M. E., Molenaar D., Konings W. N. Mechanism of Na(+)-dependent citrate transport in Klebsiella pneumoniae. J Bacteriol. 1992 Aug;174(15):4893–4898. doi: 10.1128/jb.174.15.4893-4898.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. van der Rest M. E., Schwarz E., Oesterhelt D., Konings W. N. DNA sequence of a citrate carrier of Klebsiella pneumoniae. Eur J Biochem. 1990 Apr 30;189(2):401–407. doi: 10.1111/j.1432-1033.1990.tb15502.x. [DOI] [PubMed] [Google Scholar]
  31. van der Rest M. E., Siewe R. M., Abee T., Schwarz E., Oesterhelt D., Konings W. N. Nucleotide sequence and functional properties of a sodium-dependent citrate transport system from Klebsiella pneumoniae. J Biol Chem. 1992 May 5;267(13):8971–8976. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES