Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1995 Aug;61(8):3177–3179. doi: 10.1128/aem.61.8.3177-3179.1995

Use of pulsed-field gel electrophoresis to link sporadic cases of invasive listeriosis with recalled chocolate milk.

M E Proctor 1, R Brosch 1, J W Mellen 1, L A Garrett 1, C W Kaspar 1, J B Luchansky 1
PMCID: PMC167594  PMID: 7487050

Abstract

Pulsed-field gel electrophoresis established the linkage between recalled chocolate milk and a multistate invasive listeriosis outbreak during a four-product recall period. Listeria monocytogenes isolates from four hospitalized patients and an environmental dairy sample displayed AscI restriction endonuclease digestion profiles identical to that of the chocolate milk isolate.

Full Text

The Full Text of this article is available as a PDF (292.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brosch R., Buchrieser C., Rocourt J. Subtyping of Listeria monocytogenes serovar 4b by use of low-frequency-cleavage restriction endonucleases and pulsed-field gel electrophoresis. Res Microbiol. 1991 Jul-Aug;142(6):667–675. doi: 10.1016/0923-2508(91)90080-t. [DOI] [PubMed] [Google Scholar]
  2. Brosch R., Chen J., Luchansky J. B. Pulsed-field fingerprinting of listeriae: identification of genomic divisions for Listeria monocytogenes and their correlation with serovar. Appl Environ Microbiol. 1994 Jul;60(7):2584–2592. doi: 10.1128/aem.60.7.2584-2592.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buchrieser C., Brosch R., Catimel B., Rocourt J. Pulsed-field gel electrophoresis applied for comparing Listeria monocytogenes strains involved in outbreaks. Can J Microbiol. 1993 Apr;39(4):395–401. doi: 10.1139/m93-058. [DOI] [PubMed] [Google Scholar]
  4. Danielsson-Tham M. L., Bille J., Brosch R., Buchrieser C., Persson K., Rocourt J., Schwarzkopf A., Tham W., Ursing J. Characterization of Listeria strains isolated from soft cheese. Int J Food Microbiol. 1993 Apr;18(2):161–166. doi: 10.1016/0168-1605(93)90220-b. [DOI] [PubMed] [Google Scholar]
  5. Farber J. M., Peterkin P. I. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991 Sep;55(3):476–511. doi: 10.1128/mr.55.3.476-511.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Howard P. J., Harsono K. D., Luchansky J. B. Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis. Appl Environ Microbiol. 1992 Feb;58(2):709–712. doi: 10.1128/aem.58.2.709-712.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schuchat A., Deaver K. A., Wenger J. D., Plikaytis B. D., Mascola L., Pinner R. W., Reingold A. L., Broome C. V. Role of foods in sporadic listeriosis. I. Case-control study of dietary risk factors. The Listeria Study Group. JAMA. 1992 Apr 15;267(15):2041–2045. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES