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. 1995 Aug;61(8):3185–3188. doi: 10.1128/aem.61.8.3185-3188.1995

Degradation of p-xylene by a denitrifying enrichment culture.

A Häner 1, P Höhener 1, J Zeyer 1
PMCID: PMC167596  PMID: 7487052

Abstract

Microbial cultures enriched from a diesel fuel-contaminated aquifer were able to grow on p-xylene under denitrifying conditions. The oxidation of p-xylene to CO2 was coupled to the reduction of NO3-. The enrichment cultures also grew on toluene and m-xylene, but they did not degrade benzene, ethylbenzene, and o-xylene.

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Selected References

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  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Dolfing J., Zeyer J., Binder-Eicher P., Schwarzenbach R. P. Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen. Arch Microbiol. 1990;154(4):336–341. doi: 10.1007/BF00276528. [DOI] [PubMed] [Google Scholar]
  3. Edwards E. A., Grbić-Galić D. Anaerobic degradation of toluene and o-xylene by a methanogenic consortium. Appl Environ Microbiol. 1994 Jan;60(1):313–322. doi: 10.1128/aem.60.1.313-322.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edwards E. A., Wills L. E., Reinhard M., Grbić-Galić D. Anaerobic degradation of toluene and xylene by aquifer microorganisms under sulfate-reducing conditions. Appl Environ Microbiol. 1992 Mar;58(3):794–800. doi: 10.1128/aem.58.3.794-800.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans P. J., Mang D. T., Kim K. S., Young L. Y. Anaerobic degradation of toluene by a denitrifying bacterium. Appl Environ Microbiol. 1991 Apr;57(4):1139–1145. doi: 10.1128/aem.57.4.1139-1145.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Evans P. J., Mang D. T., Young L. Y. Degradation of toluene and m-xylene and transformation of o-xylene by denitrifying enrichment cultures. Appl Environ Microbiol. 1991 Feb;57(2):450–454. doi: 10.1128/aem.57.2.450-454.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fries M. R., Zhou J., Chee-Sanford J., Tiedje J. M. Isolation, characterization, and distribution of denitrifying toluene degraders from a variety of habitats. Appl Environ Microbiol. 1994 Aug;60(8):2802–2810. doi: 10.1128/aem.60.8.2802-2810.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grbić-Galić D., Vogel T. M. Transformation of toluene and benzene by mixed methanogenic cultures. Appl Environ Microbiol. 1987 Feb;53(2):254–260. doi: 10.1128/aem.53.2.254-260.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lovley D. R., Lonergan D. J. Anaerobic Oxidation of Toluene, Phenol, and p-Cresol by the Dissimilatory Iron-Reducing Organism, GS-15. Appl Environ Microbiol. 1990 Jun;56(6):1858–1864. doi: 10.1128/aem.56.6.1858-1864.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lovley D. R., Woodward J. C., Chapelle F. H. Stimulated anoxic biodegradation of aromatic hydrocarbons using Fe(III) ligands. Nature. 1994 Jul 14;370(6485):128–131. doi: 10.1038/370128a0. [DOI] [PubMed] [Google Scholar]
  11. Rabus R., Nordhaus R., Ludwig W., Widdel F. Complete oxidation of toluene under strictly anoxic conditions by a new sulfate-reducing bacterium. Appl Environ Microbiol. 1993 May;59(5):1444–1451. doi: 10.1128/aem.59.5.1444-1451.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rabus R., Widdel F. Anaerobic degradation of ethylbenzene and other aromatic hydrocarbons by new denitrifying bacteria. Arch Microbiol. 1995 Feb;163(2):96–103. doi: 10.1007/BF00381782. [DOI] [PubMed] [Google Scholar]
  13. Schocher R. J., Seyfried B., Vazquez F., Zeyer J. Anaerobic degradation of toluene by pure cultures of denitrifying bacteria. Arch Microbiol. 1991;157(1):7–12. doi: 10.1007/BF00245327. [DOI] [PubMed] [Google Scholar]
  14. Seyfried B., Glod G., Schocher R., Tschech A., Zeyer J. Initial reactions in the anaerobic oxidation of toluene and m-xylene by denitrifying bacteria. Appl Environ Microbiol. 1994 Nov;60(11):4047–4052. doi: 10.1128/aem.60.11.4047-4052.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tschech A., Fuchs G. Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads. Arch Microbiol. 1987 Sep;148(3):213–217. doi: 10.1007/BF00414814. [DOI] [PubMed] [Google Scholar]
  16. Vogel T. M., Grbìc-Galìc D. Incorporation of Oxygen from Water into Toluene and Benzene during Anaerobic Fermentative Transformation. Appl Environ Microbiol. 1986 Jul;52(1):200–202. doi: 10.1128/aem.52.1.200-202.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zeyer J., Kuhn E. P., Schwarzenbach R. P. Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen. Appl Environ Microbiol. 1986 Oct;52(4):944–947. doi: 10.1128/aem.52.4.944-947.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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