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. 1995 Sep;61(9):3268–3273. doi: 10.1128/aem.61.9.3268-3273.1995

Suitability of selective plating media for recovering heat- or freeze-stressed Escherichia coli O157:H7 from tryptic soy broth and ground beef.

M Rocelle 1, S Clavero 1, L R Beuchat 1
PMCID: PMC167607  PMID: 7574637

Abstract

The efficacy of tryptic soy agar (TSA), modified sorbitol MacConkey agar (MSMA), modified eosin methylene blue (MEMB) agar, and modified SD-39 (MSD) agar in recovering a five-strain mixture of enterohemorrhagic Escherichia coli O157:H7 and five non-O157 strains of E. coli heated in tryptic soy broth at 52, 54, or 56 degrees C for 10, 20, and 30 min was determined. Nonselective TSA supported the highest recovery of heated cells. Significantly (P < or = 0.05) lower recovery of heat-stressed cells was observed on MSMA than on TSA, MEMB agar, or MSD agar. The suitability of MEMB agar or MSD agar for recovery of E. coli O157:H7 from heated or frozen (-20 degrees C) low- or high-fat ground beef was determined. Recovery of E. coli O157:H7 from heated ground beef was significantly (P < or = 0.05) higher on TSA than on MEMB agar, which in turn supported higher recovery than MSD agar did; MSMA was inferior. Recovery from frozen ground beef was also higher on MEMB and MSD agars than on MSMA. Higher populations were generally recovered from high-fat beef than from low-fat beef, but the relative performance of the recovery media was the same. The inability of MSMA to recover stressed cells of E. coli O157:H7 underscores the need to develop a better selective medium for enumerating E. coli O157:H7.

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Selected References

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  1. Abdul-Raouf U. M., Beuchat L. R., Ammar M. S. Survival and growth of Escherichia coli O157:H7 in ground, roasted beef as affected by pH, acidulants, and temperature. Appl Environ Microbiol. 1993 Aug;59(8):2364–2368. doi: 10.1128/aem.59.8.2364-2368.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BUSTA F. F., JEZESKI J. J. EFFECT OF SODIUM CHLORIDE CONCENTRATION IN AN AGAR MEDIUM ON GROWTH OF HEAT-SHOCKED STAPHYLOCOCCUS AUREUS. Appl Microbiol. 1963 Sep;11:404–407. doi: 10.1128/am.11.5.404-407.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Belongia E. A., MacDonald K. L., Parham G. L., White K. E., Korlath J. A., Lobato M. N., Strand S. M., Casale K. A., Osterholm M. T. An outbreak of Escherichia coli O157:H7 colitis associated with consumption of precooked meat patties. J Infect Dis. 1991 Aug;164(2):338–343. doi: 10.1093/infdis/164.2.338. [DOI] [PubMed] [Google Scholar]
  4. Beuchat L. R. Injury and repair of gram-negative bacteria, with special consideration of the involvement of the cytoplasmic membrane. Adv Appl Microbiol. 1978;23:219–243. doi: 10.1016/s0065-2164(08)70071-6. [DOI] [PubMed] [Google Scholar]
  5. Gannon V. P., King R. K., Kim J. Y., Thomas E. J. Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction. Appl Environ Microbiol. 1992 Dec;58(12):3809–3815. doi: 10.1128/aem.58.12.3809-3815.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hitchener B. J., Egan A. F. Outer-membrane damage in sublethally heated Escherichia coli K-12. Can J Microbiol. 1977 Mar;23(3):311–318. doi: 10.1139/m77-046. [DOI] [PubMed] [Google Scholar]
  7. Hurst A. Bacterial injury: a review. Can J Microbiol. 1977 Aug;23(8):935–944. doi: 10.1139/m77-139. [DOI] [PubMed] [Google Scholar]
  8. March S. B., Ratnam S. Latex agglutination test for detection of Escherichia coli serotype O157. J Clin Microbiol. 1989 Jul;27(7):1675–1677. doi: 10.1128/jcm.27.7.1675-1677.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. March S. B., Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol. 1986 May;23(5):869–872. doi: 10.1128/jcm.23.5.869-872.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Samadpour M., Liston J., Ongerth J. E., Tarr P. I. Evaluation of DNA probes for detection of Shiga-like-toxin-producing Escherichia coli in food and calf fecal samples. Appl Environ Microbiol. 1990 May;56(5):1212–1215. doi: 10.1128/aem.56.5.1212-1215.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tollison S. B., Johnson M. G. Sensitivity to bile salts of Shigella flexneri sublethally heat stressed in buffer or broth. Appl Environ Microbiol. 1985 Aug;50(2):337–341. doi: 10.1128/aem.50.2.337-341.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tomlins R. I., Vaaler G. L., Ordal Z. J. Lipid biosynthesis during the recovery of Salmonella typhimurium from thermal injury. Can J Microbiol. 1972 Jul;18(7):1015–1021. doi: 10.1139/m72-158. [DOI] [PubMed] [Google Scholar]

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