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. 1995 Sep;61(9):3460–3462. doi: 10.1128/aem.61.9.3460-3462.1995

Genotyping of rotaviruses isolated from sewage.

R Gajardo 1, N Bouchriti 1, R M Pintó 1, A Bosch 1
PMCID: PMC167622  PMID: 7574652

Abstract

Rotaviruses from environmental samples have been genotyped by a seminested reverse transcription PCR assay with serotype-specific primers derived from variable regions of gene 9, which produce different characteristic segment sizes for serotypes 1 to 4. The method enabled the detection and identification of type 1, 2, and 3 group A rotaviruses in sewage.

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Selected References

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  1. Atmar R. L., Metcalf T. G., Neill F. H., Estes M. K. Detection of enteric viruses in oysters by using the polymerase chain reaction. Appl Environ Microbiol. 1993 Feb;59(2):631–635. doi: 10.1128/aem.59.2.631-635.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495–503. doi: 10.1128/jcm.28.3.495-503.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bosch A., Pinto R. M., Jofre J. Non-seasonal distribution of rotavirus in Barcelona raw sewage. Zentralbl Bakteriol Mikrobiol Hyg B. 1988 Jun;186(3):273–277. [PubMed] [Google Scholar]
  4. Gouvea V., Glass R. I., Woods P., Taniguchi K., Clark H. F., Forrester B., Fang Z. Y. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol. 1990 Feb;28(2):276–282. doi: 10.1128/jcm.28.2.276-282.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8701–8704. doi: 10.1073/pnas.82.24.8701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kopecka H., Dubrou S., Prevot J., Marechal J., López-Pila J. M. Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization. Appl Environ Microbiol. 1993 Apr;59(4):1213–1219. doi: 10.1128/aem.59.4.1213-1219.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pintó R. M., Diez J. M., Bosch A. Use of the colonic carcinoma cell line CaCo-2 for in vivo amplification and detection of enteric viruses. J Med Virol. 1994 Nov;44(3):310–315. doi: 10.1002/jmv.1890440317. [DOI] [PubMed] [Google Scholar]
  8. Smith E. M., Gerba C. P. Development of a method for detection of human rotavirus in water and sewage. Appl Environ Microbiol. 1982 Jun;43(6):1440–1450. doi: 10.1128/aem.43.6.1440-1450.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Straub T. M., Pepper I. L., Abbaszadegan M., Gerba C. P. A method to detect enteroviruses in sewage sludge-amended soil using the PCR. Appl Environ Microbiol. 1994 Mar;60(3):1014–1017. doi: 10.1128/aem.60.3.1014-1017.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tsai Y. L., Sobsey M. D., Sangermano L. R., Palmer C. J. Simple method of concentrating enteroviruses and hepatitis A virus from sewage and ocean water for rapid detection by reverse transcriptase-polymerase chain reaction. Appl Environ Microbiol. 1993 Oct;59(10):3488–3491. doi: 10.1128/aem.59.10.3488-3491.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Woods P. A., Gentsch J., Gouvea V., Mata L., Santosham M., Bai Z. S., Urasawa S., Glass R. I. Distribution of serotypes of human rotavirus in different populations. J Clin Microbiol. 1992 Apr;30(4):781–785. doi: 10.1128/jcm.30.4.781-785.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Young C. C., Burghoff R. L., Keim L. G., Minak-Bernero V., Lute J. R., Hinton S. M. Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils. Appl Environ Microbiol. 1993 Jun;59(6):1972–1974. doi: 10.1128/aem.59.6.1972-1974.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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