Abstract
The antimicrobial effects of ozonated water in a recirculating concurrent reactor were evaluated against four gram-positive and four gram-negative bacteria, two yeasts, and spores of Aspergillus niger. More than 5 log units each of Salmonella typhimurium and Escherichia coli cells were killed instantaneously in ozonated water with or without addition of 20 ppm of soluble starch (SS). In ozonated water, death rates among the gram-negative bacteria--S. typhimurium, E. coli, Pseudomonas aeruginosa, and Yersinia enterocolitica--were not significantly different (P > 0.05). Among gram-positive bacteria, Listeria monocytogenes was significantly P < 0.05) more sensitive than either Staphylococcus aureus or Enterococcus faecalis. In the presence of organic material, death rates of S. aureus compared with L. monocytogenes and E. coli compared with S. typhimurium in ozonated water were not significantly (P > 0.05) affected by SS addition but were significantly reduced (P < 0.05) by addition of 20 ppm of bovine serum albumin (BSA). More than 4.5 log units each of Candida albicans and Zygosaccharomyces bailii cells were killed instantaneously in ozonated water, whereas less than 1 log unit of Aspergillus niger spores was killed after a 5-min exposure. The average ozone output levels in the deionized water (0.188 mg/ml) or water with SS (0.198 mg/ml) did not differ significantly (P < 0.05) but were significantly lower in water containing BSA (0.149 mg/ml).
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Selected References
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- Beuchat L. R., Brackett R. E. Behavior of Listeria monocytogenes inoculated into raw tomatoes and processed tomato products. Appl Environ Microbiol. 1991 May;57(5):1367–1371. doi: 10.1128/aem.57.5.1367-1371.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Broadwater W. T., Hoehn R. C., King P. H. Sensitivity of three selected bacterial species to ozone. Appl Microbiol. 1973 Sep;26(3):391–393. doi: 10.1128/am.26.3.391-393.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burleson G. R., Murray T. M., Pollard M. Inactivation of viruses and bacteria by ozone, with and without sonication. Appl Microbiol. 1975 Mar;29(3):340–344. doi: 10.1128/am.29.3.340-344.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FETNER R. H., INGOLS R. S. A comparison of the bactericidal activity of ozone and chlorine against Escherichia coli at 1degree. J Gen Microbiol. 1956 Oct;15(2):381–385. doi: 10.1099/00221287-15-2-381. [DOI] [PubMed] [Google Scholar]
- Finch G. R., Black E. K., Gyürék L., Belosevic M. Ozone inactivation of Cryptosporidium parvum in demand-free phosphate buffer determined by in vitro excystation and animal infectivity. Appl Environ Microbiol. 1993 Dec;59(12):4203–4210. doi: 10.1128/aem.59.12.4203-4210.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finch G. R., Black E. K., Labatiuk C. W., Gyürék L., Belosevic M. Comparison of Giardia lamblia and Giardia muris cyst inactivation by ozone. Appl Environ Microbiol. 1993 Nov;59(11):3674–3680. doi: 10.1128/aem.59.11.3674-3680.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- INGRAM M., HAINES R. B. Inhibition of bacterial growth by pure ozone in the presence of nutrients. J Hyg (Lond) 1949 Jun;47(2):146-58, tab. doi: 10.1017/s0022172400014406. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishizaki K., Shinriki N., Matsuyama H. Inactivation of Bacillus spores by gaseous ozone. J Appl Bacteriol. 1986 Jan;60(1):67–72. doi: 10.1111/j.1365-2672.1986.tb01067.x. [DOI] [PubMed] [Google Scholar]
- Jarroll E. L., Bingham A. K., Meyer E. A. Effect of chlorine on Giardia lamblia cyst viability. Appl Environ Microbiol. 1981 Feb;41(2):483–487. doi: 10.1128/aem.41.2.483-487.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Korich D. G., Mead J. R., Madore M. S., Sinclair N. A., Sterling C. R. Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocyst viability. Appl Environ Microbiol. 1990 May;56(5):1423–1428. doi: 10.1128/aem.56.5.1423-1428.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peeters J. E., Mazás E. A., Masschelein W. J., Villacorta Martiez de Maturana I., Debacker E. Effect of disinfection of drinking water with ozone or chlorine dioxide on survival of Cryptosporidium parvum oocysts. Appl Environ Microbiol. 1989 Jun;55(6):1519–1522. doi: 10.1128/aem.55.6.1519-1522.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]