Abstract
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
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- Aznar R., Ludwig W., Amann R. I., Schleifer K. H. Sequence determination of rRNA genes of pathogenic Vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probes. Int J Syst Bacteriol. 1994 Apr;44(2):330–337. doi: 10.1099/00207713-44-2-330. [DOI] [PubMed] [Google Scholar]
- Brauns L. A., Hudson M. C., Oliver J. D. Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells. Appl Environ Microbiol. 1991 Sep;57(9):2651–2655. doi: 10.1128/aem.57.9.2651-2655.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brosius J., Dull T. J., Sleeter D. D., Noller H. F. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol. 1981 May 15;148(2):107–127. doi: 10.1016/0022-2836(81)90508-8. [DOI] [PubMed] [Google Scholar]
- Desenclos J. C., Klontz K. C., Wolfe L. E., Hoecherl S. The risk of Vibrio illness in the Florida raw oyster eating population, 1981-1988. Am J Epidemiol. 1991 Aug 1;134(3):290–297. doi: 10.1093/oxfordjournals.aje.a116084. [DOI] [PubMed] [Google Scholar]
- Giesendorf B. A., Quint W. G., Henkens M. H., Stegeman H., Huf F. A., Niesters H. G. Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction. Appl Environ Microbiol. 1992 Dec;58(12):3804–3808. doi: 10.1128/aem.58.12.3804-3808.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gustafson C. E., Thomas C. J., Trust T. J. Detection of Aeromonas salmonicida from fish by using polymerase chain reaction amplification of the virulence surface array protein gene. Appl Environ Microbiol. 1992 Dec;58(12):3816–3825. doi: 10.1128/aem.58.12.3816-3825.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hill W. E., Keasler S. P., Trucksess M. W., Feng P., Kaysner C. A., Lampel K. A. Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters. Appl Environ Microbiol. 1991 Mar;57(3):707–711. doi: 10.1128/aem.57.3.707-711.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koch W. H., Payne W. L., Wentz B. A., Cebula T. A. Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods. Appl Environ Microbiol. 1993 Feb;59(2):556–560. doi: 10.1128/aem.59.2.556-560.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- León G., Maulén N., Figueroa J., Villanueva J., Rodríguez C., Vera M. I., Krauskopf M. A PCR-based assay for the identification of the fish pathogen Renibacterium salmoninarum. FEMS Microbiol Lett. 1994 Jan 15;115(2-3):131–136. doi: 10.1111/j.1574-6968.1994.tb06627.x. [DOI] [PubMed] [Google Scholar]
- O'Brien D., Mooney J., Ryan D., Powell E., Hiney M., Smith P. R., Powell R. Detection of Aeromonas salmonicida, causal agent of furunculosis in salmonid fish, from the tank effluent of hatchery-reared Atlantic salmon smolts. Appl Environ Microbiol. 1994 Oct;60(10):3874–3877. doi: 10.1128/aem.60.10.3874-3877.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oliver J. D., Nilsson L., Kjelleberg S. Formation of nonculturable Vibrio vulnificus cells and its relationship to the starvation state. Appl Environ Microbiol. 1991 Sep;57(9):2640–2644. doi: 10.1128/aem.57.9.2640-2644.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright A. C., Miceli G. A., Landry W. L., Christy J. B., Watkins W. D., Morris J. G., Jr Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe. Appl Environ Microbiol. 1993 Feb;59(2):541–546. doi: 10.1128/aem.59.2.541-546.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]