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. 1995 Oct;61(10):3549–3555. doi: 10.1128/aem.61.10.3549-3555.1995

Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field.

L Bodrossy 1, J C Murrell 1, H Dalton 1, M Kalman 1, L G Puskas 1, K L Kovacs 1
PMCID: PMC167649  PMID: 7486989

Abstract

Methanotrophic bacteria were isolated from a natural environment potentially favorable to heat-tolerant methanotrophs. An improved colony plate assay was developed and used to identify putative methanotrophic colonies with high confidence. Fourteen new isolates were purified and partially characterized. These new isolates exhibit a DNA sequence homology of up to 97% with the conserved regions in the mmoX and mmoC genes of the soluble methane monooxygenase (MMO)-coding gene cluster of Methylococcus capsulatus Bath. The copper regulation of soluble MMO expression in the same isolates, however, differs from that of M. capsulatus Bath, as the new isolates can tolerate up to 0.8 microM copper without loss of MMO activity while a drastic reduction of MMO activity occurs already at 0.1 microM copper in M. capsulatus Bath. The isolates can be cultivated and utilized at elevated temperatures, and their copper- and heat-tolerant MMO activity makes these bacteria ideal candidates for future biotechnological use.

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Selected References

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  1. Brusseau G. A., Tsien H. C., Hanson R. S., Wackett L. P. Optimization of trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity. Biodegradation. 1990;1(1):19–29. doi: 10.1007/BF00117048. [DOI] [PubMed] [Google Scholar]
  2. Cardy D. L., Laidler V., Salmond G. P., Murrell J. C. Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b. Mol Microbiol. 1991 Feb;5(2):335–342. doi: 10.1111/j.1365-2958.1991.tb02114.x. [DOI] [PubMed] [Google Scholar]
  3. Cardy D. L., Laidler V., Salmond G. P., Murrell J. C. The methane monooxygenase gene cluster of Methylosinus trichosporium: cloning and sequencing of the mmoC gene. Arch Microbiol. 1991;156(6):477–483. doi: 10.1007/BF00245395. [DOI] [PubMed] [Google Scholar]
  4. Fitch M. W., Graham D. W., Arnold R. G., Agarwal S. K., Phelps P., Speitel G. E., Jr, Georgiou G. Phenotypic characterization of copper-resistant mutants of Methylosinus trichosporium OB3b. Appl Environ Microbiol. 1993 Sep;59(9):2771–2776. doi: 10.1128/aem.59.9.2771-2776.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graham D. W., Korich D. G., LeBlanc R. P., Sinclair N. A., Arnold R. G. Applications of a colorimetric plate assay for soluble methane monooxygenase activity. Appl Environ Microbiol. 1992 Jul;58(7):2231–2236. doi: 10.1128/aem.58.7.2231-2236.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inoue H., Nojima H., Okayama H. High efficiency transformation of Escherichia coli with plasmids. Gene. 1990 Nov 30;96(1):23–28. doi: 10.1016/0378-1119(90)90336-p. [DOI] [PubMed] [Google Scholar]
  7. Koh S. C., Bowman J. P., Sayler G. S. Soluble Methane Monooxygenase Production and Trichloroethylene Degradation by a Type I Methanotroph, Methylomonas methanica 68-1. Appl Environ Microbiol. 1993 Apr;59(4):960–967. doi: 10.1128/aem.59.4.960-967.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lipscomb J. D. Biochemistry of the soluble methane monooxygenase. Annu Rev Microbiol. 1994;48:371–399. doi: 10.1146/annurev.mi.48.100194.002103. [DOI] [PubMed] [Google Scholar]
  9. Little C. D., Palumbo A. V., Herbes S. E., Lidstrom M. E., Tyndall R. L., Gilmer P. J. Trichloroethylene biodegradation by a methane-oxidizing bacterium. Appl Environ Microbiol. 1988 Apr;54(4):951–956. doi: 10.1128/aem.54.4.951-956.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Malashenko Iu R., Romanovskaia V. A., Bogachenko V. N., Shved A. D. Termofil'nye i termotolerantnye bakterii, assimiliruiushchie metan. Mikrobiologiia. 1975 Sep-Oct;44(5):855–862. [PubMed] [Google Scholar]
  11. McDonald I. R., Kenna E. M., Murrell J. C. Detection of methanotrophic bacteria in environmental samples with the PCR. Appl Environ Microbiol. 1995 Jan;61(1):116–121. doi: 10.1128/aem.61.1.116-121.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murrell J. C. Genetics and molecular biology of methanotrophs. FEMS Microbiol Rev. 1992 Jun;8(3-4):233–248. doi: 10.1111/j.1574-6968.1992.tb04990.x. [DOI] [PubMed] [Google Scholar]
  13. Oldenhuis R., Vink R. L., Janssen D. B., Witholt B. Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase. Appl Environ Microbiol. 1989 Nov;55(11):2819–2826. doi: 10.1128/aem.55.11.2819-2826.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Phelps P. A., Agarwal S. K., Speitel G. E., Georgiou G. Methylosinus trichosporium OB3b Mutants Having Constitutive Expression of Soluble Methane Monooxygenase in the Presence of High Levels of Copper. Appl Environ Microbiol. 1992 Nov;58(11):3701–3708. doi: 10.1128/aem.58.11.3701-3708.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Saunders S. E., Burke J. F. Rapid isolation of miniprep DNA for double strand sequencing. Nucleic Acids Res. 1990 Aug 25;18(16):4948–4948. doi: 10.1093/nar/18.16.4948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tsien H. C., Brusseau G. A., Hanson R. S., Waclett L. P. Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b. Appl Environ Microbiol. 1989 Dec;55(12):3155–3161. doi: 10.1128/aem.55.12.3155-3161.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tsien H. C., Hanson R. S. Soluble methane monooxygenase component B gene probe for identification of methanotrophs that rapidly degrade trichloroethylene. Appl Environ Microbiol. 1992 Mar;58(3):953–960. doi: 10.1128/aem.58.3.953-960.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wackett L. P., Gibson D. T. Rapid method for detection and quantitation of hydroxylated aromatic intermediates produced by microorganisms. Appl Environ Microbiol. 1983 Mar;45(3):1144–1147. doi: 10.1128/aem.45.3.1144-1147.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Whittenbury R., Phillips K. C., Wilkinson J. F. Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol. 1970 May;61(2):205–218. doi: 10.1099/00221287-61-2-205. [DOI] [PubMed] [Google Scholar]
  20. Wilson J. T., Wilson B. H. Biotransformation of trichloroethylene in soil. Appl Environ Microbiol. 1985 Jan;49(1):242–243. doi: 10.1128/aem.49.1.242-243.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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