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. 1995 Oct;61(10):3650–3655. doi: 10.1128/aem.61.10.3650-3655.1995

Production of Trichoderma reesei cellulases on glucose-containing media.

T Nakari-Setälä 1, M Penttilä 1
PMCID: PMC167665  PMID: 7487002

Abstract

The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.

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Selected References

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