Abstract
Two media used to detect fecal coliforms in water by membrane filtration, m-FC and m-TEC, were modified and supplemented with the chromogenic substrate 5-bromo-6-chloro-3-indoyl-beta-D-glucuronide (BCIG) and were compared for quantitative recovery of Escherichia coli. Student's t test of data from 181 water samples of sewage, rivers, lakes, and wells did not demonstrate any statistically significant differences (P = 0.05) in the enumeration of E. coli with these media. Target colonies were confirmed to be E. coli at rates of 98.6 and 97.3% by using FC-BCIG and TEC-BCIG media, respectively. Glucuronidase-negative isolates of E. coli were encountered at the same frequency (6.0%) on both media. This collaborative study demonstrated that either modified basal medium could be used successfully for detection of E. coli in various nontreated waters within 24 h.
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Selected References
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- Dufour A. P., Strickland E. R., Cabelli V. J. Membrane filter method for enumerating Escherichia coli. Appl Environ Microbiol. 1981 May;41(5):1152–1158. doi: 10.1128/aem.41.5.1152-1158.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feng P. C., Hartman P. A. Fluorogenic assays for immediate confirmation of Escherichia coli. Appl Environ Microbiol. 1982 Jun;43(6):1320–1329. doi: 10.1128/aem.43.6.1320-1329.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haines J. R., Covert T. C., Rankin C. C. Evaluation of indoxyl-beta-D-glucuronide as a chromogen in media specific for Escherichia coli. Appl Environ Microbiol. 1993 Aug;59(8):2758–2759. doi: 10.1128/aem.59.8.2758-2759.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ley A. N., Bowers R. J., Wolfe S. Indoxyl-beta-D-glucuronide, a novel chromogenic reagent for the specific detection and enumeration of Escherichia coli in environmental samples. Can J Microbiol. 1988 May;34(5):690–693. doi: 10.1139/m88-115. [DOI] [PubMed] [Google Scholar]
- Mates A., Shaffer M. Membrane filtration differentiation of E. coli from coliforms in the examination of water. J Appl Bacteriol. 1989 Sep;67(3):343–346. doi: 10.1111/j.1365-2672.1989.tb02503.x. [DOI] [PubMed] [Google Scholar]
- Sarhan H. R., Foster H. A. A rapid fluorogenic method for the detection of Escherichia coli by the production of beta-glucuronidase. J Appl Bacteriol. 1991 May;70(5):394–400. doi: 10.1111/j.1365-2672.1991.tb02955.x. [DOI] [PubMed] [Google Scholar]