Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1995 Nov;61(11):4110–4113. doi: 10.1128/aem.61.11.4110-4113.1995

Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp.

D J Saul 1, L C Williams 1, R A Reeves 1, M D Gibbs 1, P L Bergquist 1
PMCID: PMC167719  PMID: 8526526

Abstract

A gene expressing xylanase activity was isolated from a genomic library of Thermotoga sp. strain FjSS3-B.1. The sequence of the gene shows that it encodes a single domain, family 10 xylanase. The recombinant enzyme has extremely high thermal stability, activity over a relatively broad pH range, and activity on Pinus radiata kraft pulp.

Full Text

The Full Text of this article is available as a PDF (244.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gilkes N. R., Henrissat B., Kilburn D. G., Miller R. C., Jr, Warren R. A. Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev. 1991 Jun;55(2):303–315. doi: 10.1128/mr.55.2.303-315.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Grépinet O., Chebrou M. C., Béguin P. Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum. J Bacteriol. 1988 Oct;170(10):4582–4588. doi: 10.1128/jb.170.10.4582-4588.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lever M. Colorimetric and fluorometric carbohydrate determination with p-hydroxybenzoic acid hydrazide. Biochem Med. 1973 Apr;7(2):274–281. doi: 10.1016/0006-2944(73)90083-5. [DOI] [PubMed] [Google Scholar]
  5. Morris D. D., Reeves R. A., Gibbs M. D., Saul D. J., Bergquist P. L. Correction of the beta-mannanase domain of the celC pseudogene from Caldocellulosiruptor saccharolyticus and activity of the gene product on kraft pulp. Appl Environ Microbiol. 1995 Jun;61(6):2262–2269. doi: 10.1128/aem.61.6.2262-2269.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Risler J. L., Delorme M. O., Delacroix H., Henaut A. Amino acid substitutions in structurally related proteins. A pattern recognition approach. Determination of a new and efficient scoring matrix. J Mol Biol. 1988 Dec 20;204(4):1019–1029. doi: 10.1016/0022-2836(88)90058-7. [DOI] [PubMed] [Google Scholar]
  7. Ritzenthaler P., Mata-Gilsinger M. Use of uxu-lac fusion strains to study the regulation of the uxuAB operon in Escherichia coli K12. J Gen Microbiol. 1983 Nov;129(11):3335–3343. doi: 10.1099/00221287-129-11-3335. [DOI] [PubMed] [Google Scholar]
  8. Schauder B., Blöcker H., Frank R., McCarthy J. E. Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region. Gene. 1987;52(2-3):279–283. doi: 10.1016/0378-1119(87)90054-0. [DOI] [PubMed] [Google Scholar]
  9. Simpson H. D., Haufler U. R., Daniel R. M. An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga. Biochem J. 1991 Jul 15;277(Pt 2):413–417. doi: 10.1042/bj2770413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Teather R. M., Wood P. J. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol. 1982 Apr;43(4):777–780. doi: 10.1128/aem.43.4.777-780.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tremblay L., Archibald F. Production of a cloned xylanase in Bacillus cereus and its performance in kraft pulp prebleaching. Can J Microbiol. 1993 Sep;39(9):853–860. doi: 10.1139/m93-127. [DOI] [PubMed] [Google Scholar]
  12. Winterhalter C., Heinrich P., Candussio A., Wich G., Liebl W. Identification of a novel cellulose-binding domain within the multidomain 120 kDa xylanase XynA of the hyperthermophilic bacterium Thermotoga maritima. Mol Microbiol. 1995 Feb;15(3):431–444. doi: 10.1111/j.1365-2958.1995.tb02257.x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES